The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.


Biochemists, molecular biologists, microbiologists, geneticists, analytical chemists, clinical chemists; industrial researchers working on protein products; plant breeders and plant pathologists.

Table of Contents

Vectors for Cloning DNA: Production of Single-Stranded Plasmid DNA. pKUN, Vectors for the Separate Production of Both DNA Strands of Recombinant Plasmids. Restriction Site Bank Vectors for Cloning in Gram-Negative Bacteria and Yeast. Plasmids for the Selection and Analysis of Prokaryotic Promoters. A *gl DNA Protocol Based on Purification of Phage on DEAE-Cellulose. Double cos Site Vectors: Simplified Cosmid Cloning. Improved in Vitro Packaging of *gl DNA. *gl Phage Vectors--Embl Series. Plasmid and Phage Vectors for Gene Cloning and Analysis in Streptomyces. Cosmid Shuttle Vectors for Cloning and Analysis of Streptomyces DNA. Host-Vector Systems for Gene Cloning in Cyanobacteria. Genetic Engineering of the Cyanobacterial Chromosome. Conjugal Transfer of Plasmids to Cyanobacteria. Site-Directed Chromosomal Rearrangements in Yeast. Improved Vectors for Plant Transformation: Expression Cassette Vectors and New Selectable Markers. Vectors for Cloning in Plant Cells.Binary Ti Vectors for Plant Transformation and Promoter Analysis. Detection for Monocot Transformation via Agrobacterium tumefaciens. Direct Gene Transfer to Protoplasts of Dicotyledonous and Monocotyledonous Plants by a Number of Methods, Including Electroporation. Uptake of DNA and RNA into Cells Mediated by Electroporation. Electroporation of DNA and RNA into Plant Protoplasts. Cloning Vectors of Mitochondrial Origin for Eukaryotes. Vectors for Expression of Cloned Genes: Short Homopeptide Leader Sequences Enhanced Production of Human Proinsulin in Escherichia coli. Expression of Bovine Growth Hormone Derivatives in Escherichia coli and the Use of the Derivatives to


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© 1987
Academic Press
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@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY