(Section Headings): Use of Enzymes in Recombinant DNA Research. Enzymes Affecting the Gross Morphology of DNA: Topoisomerases Type I; Topoisomerases Type II. Proteins with Specialized Functions Acting at Specific Loci. New Methods for DNA Isolation, Hybridization, and Cloning. Analytical Methods for Gene Products. Mutagenesis: In Vitro and in Vivo. Author Index. Subject Index.
FROM THE PREFACE: Exciting new developments in recombinant DNA research allow the isolation and amplification of specific genes or DNA segments from almost any living organism. These new developments have revolutionized our approaches to solving complex biological problems and have opened up new possibilities for producing new and better products in the areas of health, agriculture, and industry. Volumes 100 and 101 supplement Volumes 65 and 68 of Methods in Enzymology. During the last three years, many new or improved methods on recombinant DNA or nucleic acids have appeared, and they are included in these two volumes. Volume 100 covers the use of enzymes in recombinant DNA research, enzymes affecting the gross morphology of DNA, proteins with specialized functions acting at specific loci, new methods for DNA isolation, hybridization, and cloning, analytical methods for gene products, and mutagenesis: in vitro and in vivo. Volume 101 includes sections on new vectors for cloning genes, cloning of genes into yeast cells, and systems for monitoring cloned gene expression.
- No. of pages:
- © Academic Press 1983
- 28th June 1983
- Academic Press
- eBook ISBN:
@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be Colowick and Kaplan's multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY
Vanderbilt University School of Medicine, Nashville, Tennessee, U.S.A.
Division of Biological Sciences, Cornell University, Ithaca, New York, U.S.A.
School of Hygiene and Public Health, The Johns Hopkins University, Baltimore, Maryland, U.S.A.
Department of Molecular Biology and Biochemistry, University of California, Irvine, California, U.S.A.
Elsevier.com visitor survey
We are always looking for ways to improve customer experience on Elsevier.com.
We would like to ask you for a moment of your time to fill in a short questionnaire, at the end of your visit.
If you decide to participate, a new browser tab will open so you can complete the survey after you have completed your visit to this website.
Thanks in advance for your time.