Recombinant DNA, Part B, Volume 100

1st Edition

Serial Volume Editors: Ray Wu Lawrence Grossman Kivie Moldave
Print ISBN: 9780121820008
eBook ISBN: 9780080882178
Imprint: Academic Press
Published Date: 28th June 1983
Page Count: 540


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FROM THE PREFACE: Exciting new developments in recombinant DNA research allow the isolation and amplification of specific genes or DNA segments from almost any living organism. These new developments have revolutionized our approaches to solving complex biological problems and have opened up new possibilities for producing new and better products in the areas of health, agriculture, and industry. Volumes 100 and 101 supplement Volumes 65 and 68 of Methods in Enzymology. During the last three years, many new or improved methods on recombinant DNA or nucleic acids have appeared, and they are included in these two volumes. Volume 100 covers the use of enzymes in recombinant DNA research, enzymes affecting the gross morphology of DNA, proteins with specialized functions acting at specific loci, new methods for DNA isolation, hybridization, and cloning, analytical methods for gene products, and mutagenesis: in vitro and in vivo. Volume 101 includes sections on new vectors for cloning genes, cloning of genes into yeast cells, and systems for monitoring cloned gene expression.

Table of Contents

(Section Headings): Use of Enzymes in Recombinant DNA Research. Enzymes Affecting the Gross Morphology of DNA: Topoisomerases Type I; Topoisomerases Type II. Proteins with Specialized Functions Acting at Specific Loci. New Methods for DNA Isolation, Hybridization, and Cloning. Analytical Methods for Gene Products. Mutagenesis: In Vitro and in Vivo. Author Index. Subject Index.


No. of pages:
© Academic Press 1983
Academic Press
eBook ISBN:
Hardcover ISBN:

About the Serial Volume Editor

Ray Wu

Affiliations and Expertise

Division of Biological Sciences, Cornell University, Ithaca, New York, U.S.A.

Lawrence Grossman

Affiliations and Expertise

School of Hygiene and Public Health, The Johns Hopkins University, Baltimore, Maryland, U.S.A.

Kivie Moldave

Affiliations and Expertise

Department of Molecular Biology and Biochemistry, University of California, Irvine, California, U.S.A.


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