Recombinant DNA And Cell Proliferation

Recombinant DNA And Cell Proliferation

1st Edition - May 1, 1984

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  • Editor: Gary Stein
  • eBook ISBN: 9780323153362

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Recombinant DNA and Cell Proliferation focuses on the use of recombinant DNA technology in investigating the regulation of cell proliferation. Topics include gene transfer for assessing the role of defined DNA sequences in triggering DNA replication, nucleic acid hybridization probes for analyzing the regulation of specific genes during the cell cycle, and cloned DNAs for studying genes expressed with proliferation and differentiation. This book is organized into three sections encompassing 13 chapters and begins with a discussion on the expression of specific genes during the cell cycle. This text also deals with topics such as the use of cloned SV40 DNA fragments to examine signals for cell proliferation, expression of dihydrofolate reductase and thymidylate synthase genes in mammalian cells, and gene expression during the cell cycle of Chlamydomonas reinhardtii. The following chapters explore the expression of histone genes during the cell cycle in human cells; organization and expression of eukaryotic ribosomal protein genes; and expression of the alpha-fetoprotein gene during development, regeneration, and carcinogenesis. This book also introduces the reader to the role of the cell division cycle in induced differentiation, gene regulation in muscle cells, regulation of nonmuscle actin gene expression during early development, and sequences at ends of cellular DNA molecules in relation to telomere replication and function. An overview of the biochemical aspects of cell proliferation and the genes and gene products that are necessary and specific for cell proliferation concludes the book. This book will be of value both to advanced students and to research scientists.

Table of Contents

  • Contributors


    I Expression of Specific Genes during the Cell Cycle

    1 Use of Cloned SV40 DNA Fragments to Study Signals for Cell Proliferation

    I. Introduction

    II. Gene Transfer by Manual Microinjection

    III. SV40 and Cell Proliferation

    IV. A Growing Cell Receives Signals for Growth in Size

    V. A Growing Cell Receives Signals to Replicate DNA

    VI. Evidence That Signals for Growth in Size Can Be Separated from Signals for Cell DNA Replication

    VII. The Information for Growth in Size and for Cell DNA Replication Is Encoded in Different DNA Sequences of the SV40 A Gene

    VIII. Discussion


    2 Expression of Dihydrofolate Reductase and Thymidylate Synthase Genes in Mammalian Cells

    I. Overview

    II. Regulation of DHFR, TS, and TK Enzyme Levels in Normal Cells

    III. Isolation and Characterization of DHFR-Overproducing Cell Lines

    IV. DHFR Gene Expression in Overproducing Cells

    V. Isolation and Characterization of TS-Overproducing Cells


    3 Enzyme Expression during Growth and Cell Division in Saccharomyces cerevisiae: A Study of Galactose and Phosphorus Metabolism

    I. Introduction

    II. Regulation in the Galactose Metabolic Pathway

    III. Regulation of Phosphorus Metabolism

    IV. Concluding Remarks


    4 Gene Expression during the Cell Cycle of Chlamydomonas reinhardtii

    I. Introduction: Chlamydomonas as an Experimental Organism

    II. Characteristics of Synchronous Cultures of C. reinhardtii

    III. Expression of rRNA Genes during the Cell Cycle

    IV. Expression of Tubulin Genes during the Cell Cycle

    V. Ribulose-1,5-bisphosphate Carboxylase Synthesis during the Cell Cycle

    VI. Synthesis of Photosynthetic Membrane Proteins during the Cell Cycle

    VII. Conclusion


    5 Expression of Histone Genes during the Cell Cycle in Human Cells

    I. Introduction

    II. Structure and Organization of Human Histone Genes

    III. Levels of Control of Human Histone Gene Expression

    IV. Relationship between Histone Gene Expression and DNA Replication

    V. Conclusion


    6 Expression of the α-Fetoprotein Gene during Development, Regeneration, and Carcinogenesis

    I. Introduction

    II. Structure of the α-Fetoprotein Gene

    III. Methylation of the α-Fetoprotein Gene

    IV. Stability of α-Fetoprotein mRNA

    V. α-Fetoprotein mRNA during Development

    VI. α-Fetoprotein mRNA in Liver Regeneration and Neoplasia


    7 Organization and Expression of Eukaryotic Ribosomal Genes

    I. Introduction

    II. Isolation of Eukaryotic Ribosomal Protein Genes

    III. Characteristics of Eukaryotic Ribosomal Protein Genes

    IV. Metabolism of Ribosomal Protein

    V. Future Prospects


    II Expression of Specific Genes Associated with Proliferation and Differentiation

    8 Cell Cycle Dependence of Globin Gene Expression in Friend Cells

    I. Introduction

    II. Murine Erythroleukemia Cells (Friend Cells)

    III. Role of the Cell Cycle in Friend Cell Differentiation

    IV. Friend Cell Variants Temperature Sensitive for Growth

    V. Cell Cycle-Dependent Hemoglobin Synthesis in Temperature-Sensitive Friend Cells

    VI. Commitment: The Decision to Differentiate

    VII. Significance of the Cell Cycle during Commitment to Differentiation

    VIII. Conclusions and Future Prospects


    9 Proliferation, Differentiation, and Gene Regulation in Skeletal Muscle Myogenesis: Recombinant DNA Approaches

    I. Introduction

    II. Background

    III. Gene Regulation during Myogenesis

    IV. Control of Myogenesis: The Role of Mitogens

    V. Future Lines of Inquiry

    VI. Summary


    10 Ribosomal Protein Gene Expression in Proliferating and Nonproliferating Cells

    I. Introduction

    II. Construction and Identification of cDNA for Eukaryotic Ribosomal Proteins

    III. Organization of Ribosomal Protein Genes

    IV. Ribosomal Protein mRNA: Size, Abundance, and Stability

    V. Ribosomal Protein Synthesis in Regenerating Liver

    VI. Ribosomal Protein Synthesis in Resting and Growing Cells

    VII. Ribosomal Protein Synthesis during Development

    VIII. Concluding Remarks


    11 Regulation of Nonmuscle Actin Gene Expression during Early Development

    I. Introduction

    II. Organization of the Actin Gene Family

    III. Developmental Expression of Actin Genes


    12 Functional Architecture at Telomeres of Linear DNA in Eukaryotes

    I. Introduction: Chromosomal and Extrachromosomal Linear DNA

    II. DNA Termini Resulting from Genomic Fragmentation in Ciliated Protozoa

    III. Cloned Termini of Palindromic rDNA Molecules

    IV. Models for Telomere Function


    III Overview

    13 Recombinant DNA Approaches to Studying Control of Cell Proliferation: An Overview

    I. Introduction

    II. Mathematical Models and Kinetics of Cell Proliferation

    III. Biochemistry of Cell Proliferation

    IV. The Role of RNA Polymerase II

    V. The Search for Genes

    VI. The p53 Protein

    VII. Cell Size and the Role of Ribosomal RNA

    VIII. Conclusions



Product details

  • No. of pages: 378
  • Language: English
  • Copyright: © Academic Press 1984
  • Published: May 1, 1984
  • Imprint: Academic Press
  • eBook ISBN: 9780323153362

About the Editor

Gary Stein

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