Receptor-Receptor Interactions

Series Page

Contributors

Preface

References

Chapter 1. Spatial Intensity Distribution Analysis (SpIDA): A New Tool for Receptor Tyrosine Kinase Activation and Transactivation Quantification

Abstract

Introduction

1.1 Theory of Spatial Intensity Distribution Analysis

1.2 SpIDA: Examples of Application to RTK

1.3 Procedure for SpIDA

1.4 Discussion

Acknowledgments

References

Chapter 2. Dimerization of Nuclear Receptors

Abstract

Introduction

2.1 Methods

Acknowledgments

References

Chapter 3. Network Analysis to Uncover the Structural Communication in GPCRs

Abstract

Introduction

3.1 Materials

3.2 Methods

3.3 Discussion

Summary

Acknowledgments

References

Chapter 4. Simulating G Protein-Coupled Receptors in Native-Like Membranes: From Monomers to Oligomers

Abstract

Introduction

4.1 Membranes

4.2 GPCR Monomers in Membranes

4.3 GPCR Dimers and Oligomers in Membranes

References

Further Reading

Chapter 5. Structure-Based Molecular Modeling Approaches to GPCR Oligomerization

Abstract

Introduction

5.1 Protein–Protein Docking

5.2 MD Simulation

5.3 Normal Mode Analysis

5.4 Electrostatics Studies

Acknowledgments

References

Chapter 6. Biochemical and Imaging Methods to Study Receptor Membrane Organization and Association with Lipid Rafts

Abstract

Introduction and Rationale

6.1 Methods to Determine Protein Association with Lipid Rafts

6.2 Optical Techniques to Study Receptor Membrane Organization and Association with Lipid Rafts

Concluding Remarks

Acknowledgments

References

Chapter 7. Serotonin Type 4 Receptor Dimers

Abstract

Introduction

7.1 Materials

7.2 Methods

7.3 Discussion

Summary

Acknowledgments

References

Chapter 8. Bioluminescence Resonance Energy Transfer Methods to Study G Protein-Coupled Receptor–Receptor Tyrosine Kinase Heteroreceptor Complexes

Abstract

Introduction

Acknowledgments

References

Chapter 9. A Simple Method to Detect Allostery in GPCR Dimers

Abstract

Introduction and Rationale

Summary

Acknowledgments

References

Chapter 10. Fluorescence Correlation Spectroscopy and Photon-Counting Histogram Analysis of Receptor–Receptor Interactions

Abstract

Introduction

10.1 Materials

10.2 Methods

10.3 Discussion

Summary

Acknowledgments

References

Chapter 11. Monitoring Receptor Oligomerization by Line-Scan Fluorescence Cross-Correlation Spectroscopy

Abstract

Introduction

11.1 Materials

11.2 Methods

11.3 Discussion

Acknowledgments

References

Chapter 12. Biochemical Assay of G Protein-Coupled Receptor Oligomerization: Adenosine A₁ and Thromboxane A₂ Receptors Form the Novel Functional Hetero-oligomer

Abstract

Introduction

Conclusion

References

Chapter 13. Oligomerization of Sweet and Bitter Taste Receptors

Abstract

Introduction and Rationale

13.1 Materials

13.2 Methods

13.3 Discussion

Summary

Acknowledgments

References

Chapter 14. Analysis of Receptor–Receptor Interaction by Combined Application of FRET and Microscopy

Abstract

Introduction and Rationale

14.1 Theory

14.2 Materials / Experimental Setups

14.3 Methods (Protocols and Procedure)

14.4 Results and Discussion

Acknowledgments

References

Chapter 15. Site-Specific Labeling of Genetically Encoded Azido Groups for Multicolor, Single-Molecule Fluorescence Imaging of GPCRs

Abstract

15.1 Purpose

15.2 Theory

15.3 Protocol 1: Genetically Encoded Azido Groups for Bioorthogonal Conjugation

15.4 Protocol 2: Labeling with Fluorescent Probes Using Cyclooctynes

15.5 Protocol 3: Preparation of Biotinylated Antibodies

15.6 Protocol 4: Preparation of Detergent-Solubilized Lipids

15.7 Protocol 5: Single-Molecule Immunoprecipitation on Glass-Bottom Microplates

15.8 Protocol 6: Automated Multicolor, Single-Molecule TIRF Microscopy

Acknowledgments

References

Chapter 16. Analysis of EGF Receptor Oligomerization by Homo-FRET

Abstract

Introduction

16.1 Theory Homo-FRET Quantification

16.2 Materials

16.3 Methods

16.4 Discussion

Acknowledgments

References

Chapter 17. Detection of G Protein-Coupled Receptor (GPCR) Dimerization by Coimmunoprecipitation

Abstract

Introduction

17.1 Materials

17.2 Methods

17.3 Discussion

Acknowledgments

References

Further Reading

Chapter 18. Lipid-Dependent GPCR Dimerization

Abstract

Introduction

18.1 Materials and Method

18.2 FRET Measurements and Analysis

18.3 Interpreting Results

Summary

References

Chapter 19. Monitoring Peripheral Protein Oligomerization on Biological Membranes

Abstract

Introduction

19.1 Materials and Methods

19.2 Considerations

Summary and Conclusion

Acknowledgments

References

Chapter 20. Single-Molecule Imaging of Receptor–Receptor Interactions

Abstract

Introduction

20.1 Receptor Expression and Fluorescent Labeling

20.2 Single-Molecule Imaging

20.3 Data Analysis

Acknowledgments

References

Chapter 21. Visualization of TCR Nanoclusters via Immunogold Labeling, Freeze-Etching, and Surface Replication

Abstract

Introduction

21.1 Materials

21.2 Equipment

21.3 Methods

21.4 Analysis

21.5 Considerations

Acknowledgments

References

Chapter 22. Identification of Multimolecular Complexes and Supercomplexes in Compartment-Selective Membrane Microdomains

Abstract

Introduction and Rationale

22.1 Detergent-Insoluble Glycosphingolipid (DIG) Microdomain Isolation

22.2 Plasma Membrane Isolation

22.3 Cardiolipin-Enriched Mitochondrial Membrane Microdomain Isolation

22.4 Mitochondrial Supercomplex Identification By Blue-Native Gel Electrophoresis

22.5 Discussion

Summary

Acknowledgments

References

Chapter 23. G Protein-Coupled Receptor Transactivation: From Molecules to Mice

Abstract

Introduction

23.1 Materials

23.2 Methods

23.3 Discussion

Summary

Acknowledgments

References

Chapter 24. Crystallization of G Protein-Coupled Receptors

Abstract

Abbreviations

Introduction

24.1 Crystallization of Bovine Rhodopsin

24.2 Crystallization of β2-AR

24.3 Discussion

Acknowledgments

References

Index

Volumes in Series