Receptor-Receptor Interactions - 1st Edition - ISBN: 9780124081437, 9780124104709

Receptor-Receptor Interactions, Volume 117

1st Edition

Editors: P. Michael Conn
eBook ISBN: 9780124104709
Hardcover ISBN: 9780124081437
Imprint: Academic Press
Published Date: 28th October 2013
Page Count: 538
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Table of Contents

Series Page

Contributors

Preface

References

Chapter 1. Spatial Intensity Distribution Analysis (SpIDA): A New Tool for Receptor Tyrosine Kinase Activation and Transactivation Quantification

Abstract

Introduction

1.1 Theory of Spatial Intensity Distribution Analysis

1.2 SpIDA: Examples of Application to RTK

1.3 Procedure for SpIDA

1.4 Discussion

Acknowledgments

References

Chapter 2. Dimerization of Nuclear Receptors

Abstract

Introduction

2.1 Methods

Acknowledgments

References

Chapter 3. Network Analysis to Uncover the Structural Communication in GPCRs

Abstract

Introduction

3.1 Materials

3.2 Methods

3.3 Discussion

Summary

Acknowledgments

References

Chapter 4. Simulating G Protein-Coupled Receptors in Native-Like Membranes: From Monomers to Oligomers

Abstract

Introduction

4.1 Membranes

4.2 GPCR Monomers in Membranes

4.3 GPCR Dimers and Oligomers in Membranes

References

Further Reading

Chapter 5. Structure-Based Molecular Modeling Approaches to GPCR Oligomerization

Abstract

Introduction

5.1 Protein–Protein Docking

5.2 MD Simulation

5.3 Normal Mode Analysis

5.4 Electrostatics Studies

Acknowledgments

References

Chapter 6. Biochemical and Imaging Methods to Study Receptor Membrane Organization and Association with Lipid Rafts

Abstract

Introduction and Rationale

6.1 Methods to Determine Protein Association with Lipid Rafts

6.2 Optical Techniques to Study Receptor Membrane Organization and Association with Lipid Rafts

Concluding Remarks

Acknowledgments

References

Chapter 7. Serotonin Type 4 Receptor Dimers

Abstract

Introduction

7.1 Materials

7.2 Methods

7.3 Discussion

Summary

Acknowledgments

References

Chapter 8. Bioluminescence Resonance Energy Transfer Methods to Study G Protein-Coupled Receptor–Receptor Tyrosine Kinase Heteroreceptor Complexes

Abstract

Introduction

Acknowledgments

References

Chapter 9. A Simple Method to Detect Allostery in GPCR Dimers

Abstract

Introduction and Rationale

Summary

Acknowledgments

References

Chapter 10. Fluorescence Correlation Spectroscopy and Photon-Counting Histogram Analysis of Receptor–Receptor Interactions

Abstract

Introduction

10.1 Materials

10.2 Methods

10.3 Discussion

Summary

Acknowledgments

References

Chapter 11. Monitoring Receptor Oligomerization by Line-Scan Fluorescence Cross-Correlation Spectroscopy

Abstract

Introduction

11.1 Materials

11.2 Methods

11.3 Discussion

Acknowledgments

References

Chapter 12. Biochemical Assay of G Protein-Coupled Receptor Oligomerization: Adenosine A1 and Thromboxane A2 Receptors Form the Novel Functional Hetero-oligomer

Abstract

Introduction

Conclusion

References

Chapter 13. Oligomerization of Sweet and Bitter Taste Receptors

Abstract

Introduction and Rationale

13.1 Materials

13.2 Methods

13.3 Discussion

Summary

Acknowledgments

References

Chapter 14. Analysis of Receptor–Receptor Interaction by Combined Application of FRET and Microscopy

Abstract

Introduction and Rationale

14.1 Theory

14.2 Materials / Experimental Setups

14.3 Methods (Protocols and Procedure)

14.4 Results and Discussion

Acknowledgments

References

Chapter 15. Site-Specific Labeling of Genetically Encoded Azido Groups for Multicolor, Single-Molecule Fluorescence Imaging of GPCRs

Abstract

15.1 Purpose

15.2 Theory

15.3 Protocol 1: Genetically Encoded Azido Groups for Bioorthogonal Conjugation

15.4 Protocol 2: Labeling with Fluorescent Probes Using Cyclooctynes

15.5 Protocol 3: Preparation of Biotinylated Antibodies

15.6 Protocol 4: Preparation of Detergent-Solubilized Lipids

15.7 Protocol 5: Single-Molecule Immunoprecipitation on Glass-Bottom Microplates

15.8 Protocol 6: Automated Multicolor, Single-Molecule TIRF Microscopy

Acknowledgments

References

Chapter 16. Analysis of EGF Receptor Oligomerization by Homo-FRET

Abstract

Introduction

16.1 Theory Homo-FRET Quantification

16.2 Materials

16.3 Methods

16.4 Discussion

Acknowledgments

References

Chapter 17. Detection of G Protein-Coupled Receptor (GPCR) Dimerization by Coimmunoprecipitation

Abstract

Introduction

17.1 Materials

17.2 Methods

17.3 Discussion

Acknowledgments

References

Further Reading

Chapter 18. Lipid-Dependent GPCR Dimerization

Abstract

Introduction

18.1 Materials and Method

18.2 FRET Measurements and Analysis

18.3 Interpreting Results

Summary

References

Chapter 19. Monitoring Peripheral Protein Oligomerization on Biological Membranes

Abstract

Introduction

19.1 Materials and Methods

19.2 Considerations

Summary and Conclusion

Acknowledgments

References

Chapter 20. Single-Molecule Imaging of Receptor–Receptor Interactions

Abstract

Introduction

20.1 Receptor Expression and Fluorescent Labeling

20.2 Single-Molecule Imaging

20.3 Data Analysis

Acknowledgments

References

Chapter 21. Visualization of TCR Nanoclusters via Immunogold Labeling, Freeze-Etching, and Surface Replication

Abstract

Introduction

21.1 Materials

21.2 Equipment

21.3 Methods

21.4 Analysis

21.5 Considerations

Acknowledgments

References

Chapter 22. Identification of Multimolecular Complexes and Supercomplexes in Compartment-Selective Membrane Microdomains

Abstract

Introduction and Rationale

22.1 Detergent-Insoluble Glycosphingolipid (DIG) Microdomain Isolation

22.2 Plasma Membrane Isolation

22.3 Cardiolipin-Enriched Mitochondrial Membrane Microdomain Isolation

22.4 Mitochondrial Supercomplex Identification By Blue-Native Gel Electrophoresis

22.5 Discussion

Summary

Acknowledgments

References

Chapter 23. G Protein-Coupled Receptor Transactivation: From Molecules to Mice

Abstract

Introduction

23.1 Materials

23.2 Methods

23.3 Discussion

Summary

Acknowledgments

References

Chapter 24. Crystallization of G Protein-Coupled Receptors

Abstract

Abbreviations

Introduction

24.1 Crystallization of Bovine Rhodopsin

24.2 Crystallization of β2-AR

24.3 Discussion

Acknowledgments

References

Index

Volumes in Series


Description

This new volume of Methods in Cell Biology looks at receptor-receptor interactions, with sections on allosteric and effector interactions, crystallization and modeling, measuring receptor-receptor interactions and oligomerization in individual classes.

With cutting-edge material, this comprehensive collection is intended to guide researchers of receptor-receptor interactions for years to come.

Key Features

  • Covers sections on allosteric and effector interactions, crystallization and modeling, measuring receptor-receptor interactions and oligomerization in individual classes
  • Chapters are written by experts in the field
  • Cutting-edge material

Readership

Researchers and students in cell, molecular and developmental biology


Details

No. of pages:
538
Language:
English
Copyright:
© Academic Press 2013
Published:
Imprint:
Academic Press
eBook ISBN:
9780124104709
Hardcover ISBN:
9780124081437

About the Editors

P. Michael Conn Editor

P. Michael Conn is the Senior Vice President for Research and Associate Provost, Texas Tech Health Sciences Center. He is The Robert C. Kimbrough, Professor of Internal Medicine and Cell Biology/Biochemistry. He was previously Director of Research Advocacy and Professor of Physiology and Pharmacology, Cell Biology and Development and Obstetrics and Gynecology at Oregon Health and Science University and Senior Scientist of the Oregon National Primate Research Center (ONPRC). He served for twelve years as Special Assistant to the President and Associate Director of the ONPRC. After receiving a B.S. degree and teaching certification from the University of Michigan (1971), a M.S. from North Carolina State University (1973), and a Ph.D. degree from Baylor College of Medicine (1976), Conn did a fellowship at the NIH, then joined the faculty in the Department of Pharmacology, Duke University Medical Center where he was promoted to Associate Professor in 1982. In 1984, he became Professor and Head of Pharmacology at the University of Iowa College of Medicine, a position he held for eleven years. Conn is known for his research in the area of the cellular and molecular basis of action of gonadotropin releasing hormone action in the pituitary and therapeutic approaches that restore misfolded proteins to function. His work has led to drugs that have benefitted humans and animals. Most recently, he has identified a new class of drugs, pharmacoperones, which act by regulating the intracellular trafficking of receptors, enzymes and ion channels. He has authored or co-authored over 350 publications in this area and written or edited over 200 books, including texts in neurosciences, molecular biology and endocrinology. Conn has served as the editor of many professional journals and book series (Endocrinology, Journal of Clinical Endocrinology and Metabolism, Endocrine, Methods, Progress in Molecular Biology and Translational Science and Contemporary Endocrinology). Conn served on the National Board of Medical Examiners, including two years as chairman of the reproduction and endocrinology committee. The work of his laboratory has been recognized with a MERIT award from the NIH, the J.J. Abel Award of the American Society for Pharmacology and Experimental Therapeutics, the Weitzman, Oppenheimer and Ingbar Awards of the Endocrine Society, the National Science Medal of Mexico (the Miguel Aleman Prize) and the Stevenson Award of Canada. He is the recipient of the Oregon State Award for Discovery, the Media Award of the American College of Neuropsychopharmacology and was named a distinguished Alumnus of Baylor College of Medicine in 2012. Conn is a previous member of Council for the American Society for Cell Biology and the Endocrine Society and is a prior President of the Endocrine Society, during which time he founded the Hormone Foundation and worked with political leadership to heighten the public’s awareness of diabetes. Conn’s students and fellows have gone on to become leaders in industry and academia. He is an elected member of the Mexican Institute of Medicine and a fellow of the American Association for the Advancement of Science. He is the co-author of The Animal Research War (2008) and many articles for the public and academic community on the value of animal research and the dangers posed by animal extremism. His op/eds have appeared in The Washington Post, The LA Times, The Wall Street Journal, the Des Moines Register, and elsewhere. Conn consults with organizations that are influenced by animal extremism and with universities and companies facing challenges from these groups.

Affiliations and Expertise

Texas Tech University Health Sciences Center, Lubbock, USA