Receptor-Receptor Interactions

Receptor-Receptor Interactions

1st Edition - October 18, 2013

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  • Editor: P. Michael Conn
  • eBook ISBN: 9780124104709
  • Hardcover ISBN: 9780124081437

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Description

This new volume of Methods in Cell Biology looks at receptor-receptor interactions, with sections on allosteric and effector interactions, crystallization and modeling, measuring receptor-receptor interactions and oligomerization in individual classes. With cutting-edge material, this comprehensive collection is intended to guide researchers of receptor-receptor interactions for years to come.

Key Features

  • Covers sections on allosteric and effector interactions, crystallization and modeling, measuring receptor-receptor interactions and oligomerization in individual classes
  • Chapters are written by experts in the field
  • Cutting-edge material

Readership

Researchers and students in cell, molecular and developmental biology

Table of Contents

  • Series Page

    Contributors

    Preface

    References

    Chapter 1. Spatial Intensity Distribution Analysis (SpIDA): A New Tool for Receptor Tyrosine Kinase Activation and Transactivation Quantification

    Abstract

    Introduction

    1.1 Theory of Spatial Intensity Distribution Analysis

    1.2 SpIDA: Examples of Application to RTK

    1.3 Procedure for SpIDA

    1.4 Discussion

    Acknowledgments

    References

    Chapter 2. Dimerization of Nuclear Receptors

    Abstract

    Introduction

    2.1 Methods

    Acknowledgments

    References

    Chapter 3. Network Analysis to Uncover the Structural Communication in GPCRs

    Abstract

    Introduction

    3.1 Materials

    3.2 Methods

    3.3 Discussion

    Summary

    Acknowledgments

    References

    Chapter 4. Simulating G Protein-Coupled Receptors in Native-Like Membranes: From Monomers to Oligomers

    Abstract

    Introduction

    4.1 Membranes

    4.2 GPCR Monomers in Membranes

    4.3 GPCR Dimers and Oligomers in Membranes

    References

    Further Reading

    Chapter 5. Structure-Based Molecular Modeling Approaches to GPCR Oligomerization

    Abstract

    Introduction

    5.1 Protein–Protein Docking

    5.2 MD Simulation

    5.3 Normal Mode Analysis

    5.4 Electrostatics Studies

    Acknowledgments

    References

    Chapter 6. Biochemical and Imaging Methods to Study Receptor Membrane Organization and Association with Lipid Rafts

    Abstract

    Introduction and Rationale

    6.1 Methods to Determine Protein Association with Lipid Rafts

    6.2 Optical Techniques to Study Receptor Membrane Organization and Association with Lipid Rafts

    Concluding Remarks

    Acknowledgments

    References

    Chapter 7. Serotonin Type 4 Receptor Dimers

    Abstract

    Introduction

    7.1 Materials

    7.2 Methods

    7.3 Discussion

    Summary

    Acknowledgments

    References

    Chapter 8. Bioluminescence Resonance Energy Transfer Methods to Study G Protein-Coupled Receptor–Receptor Tyrosine Kinase Heteroreceptor Complexes

    Abstract

    Introduction

    Acknowledgments

    References

    Chapter 9. A Simple Method to Detect Allostery in GPCR Dimers

    Abstract

    Introduction and Rationale

    Summary

    Acknowledgments

    References

    Chapter 10. Fluorescence Correlation Spectroscopy and Photon-Counting Histogram Analysis of Receptor–Receptor Interactions

    Abstract

    Introduction

    10.1 Materials

    10.2 Methods

    10.3 Discussion

    Summary

    Acknowledgments

    References

    Chapter 11. Monitoring Receptor Oligomerization by Line-Scan Fluorescence Cross-Correlation Spectroscopy

    Abstract

    Introduction

    11.1 Materials

    11.2 Methods

    11.3 Discussion

    Acknowledgments

    References

    Chapter 12. Biochemical Assay of G Protein-Coupled Receptor Oligomerization: Adenosine A1 and Thromboxane A2 Receptors Form the Novel Functional Hetero-oligomer

    Abstract

    Introduction

    Conclusion

    References

    Chapter 13. Oligomerization of Sweet and Bitter Taste Receptors

    Abstract

    Introduction and Rationale

    13.1 Materials

    13.2 Methods

    13.3 Discussion

    Summary

    Acknowledgments

    References

    Chapter 14. Analysis of Receptor–Receptor Interaction by Combined Application of FRET and Microscopy

    Abstract

    Introduction and Rationale

    14.1 Theory

    14.2 Materials / Experimental Setups

    14.3 Methods (Protocols and Procedure)

    14.4 Results and Discussion

    Acknowledgments

    References

    Chapter 15. Site-Specific Labeling of Genetically Encoded Azido Groups for Multicolor, Single-Molecule Fluorescence Imaging of GPCRs

    Abstract

    15.1 Purpose

    15.2 Theory

    15.3 Protocol 1: Genetically Encoded Azido Groups for Bioorthogonal Conjugation

    15.4 Protocol 2: Labeling with Fluorescent Probes Using Cyclooctynes

    15.5 Protocol 3: Preparation of Biotinylated Antibodies

    15.6 Protocol 4: Preparation of Detergent-Solubilized Lipids

    15.7 Protocol 5: Single-Molecule Immunoprecipitation on Glass-Bottom Microplates

    15.8 Protocol 6: Automated Multicolor, Single-Molecule TIRF Microscopy

    Acknowledgments

    References

    Chapter 16. Analysis of EGF Receptor Oligomerization by Homo-FRET

    Abstract

    Introduction

    16.1 Theory Homo-FRET Quantification

    16.2 Materials

    16.3 Methods

    16.4 Discussion

    Acknowledgments

    References

    Chapter 17. Detection of G Protein-Coupled Receptor (GPCR) Dimerization by Coimmunoprecipitation

    Abstract

    Introduction

    17.1 Materials

    17.2 Methods

    17.3 Discussion

    Acknowledgments

    References

    Further Reading

    Chapter 18. Lipid-Dependent GPCR Dimerization

    Abstract

    Introduction

    18.1 Materials and Method

    18.2 FRET Measurements and Analysis

    18.3 Interpreting Results

    Summary

    References

    Chapter 19. Monitoring Peripheral Protein Oligomerization on Biological Membranes

    Abstract

    Introduction

    19.1 Materials and Methods

    19.2 Considerations

    Summary and Conclusion

    Acknowledgments

    References

    Chapter 20. Single-Molecule Imaging of Receptor–Receptor Interactions

    Abstract

    Introduction

    20.1 Receptor Expression and Fluorescent Labeling

    20.2 Single-Molecule Imaging

    20.3 Data Analysis

    Acknowledgments

    References

    Chapter 21. Visualization of TCR Nanoclusters via Immunogold Labeling, Freeze-Etching, and Surface Replication

    Abstract

    Introduction

    21.1 Materials

    21.2 Equipment

    21.3 Methods

    21.4 Analysis

    21.5 Considerations

    Acknowledgments

    References

    Chapter 22. Identification of Multimolecular Complexes and Supercomplexes in Compartment-Selective Membrane Microdomains

    Abstract

    Introduction and Rationale

    22.1 Detergent-Insoluble Glycosphingolipid (DIG) Microdomain Isolation

    22.2 Plasma Membrane Isolation

    22.3 Cardiolipin-Enriched Mitochondrial Membrane Microdomain Isolation

    22.4 Mitochondrial Supercomplex Identification By Blue-Native Gel Electrophoresis

    22.5 Discussion

    Summary

    Acknowledgments

    References

    Chapter 23. G Protein-Coupled Receptor Transactivation: From Molecules to Mice

    Abstract

    Introduction

    23.1 Materials

    23.2 Methods

    23.3 Discussion

    Summary

    Acknowledgments

    References

    Chapter 24. Crystallization of G Protein-Coupled Receptors

    Abstract

    Abbreviations

    Introduction

    24.1 Crystallization of Bovine Rhodopsin

    24.2 Crystallization of β2-AR

    24.3 Discussion

    Acknowledgments

    References

    Index

    Volumes in Series

Product details

  • No. of pages: 538
  • Language: English
  • Copyright: © Academic Press 2013
  • Published: October 18, 2013
  • Imprint: Academic Press
  • eBook ISBN: 9780124104709
  • Hardcover ISBN: 9780124081437

About the Editor

P. Michael Conn

P. Michael Conn is the Senior Vice President for Research and Associate Provost, Texas Tech Health Sciences Center. He is The Robert C. Kimbrough, Professor of Internal Medicine and Cell Biology/Biochemistry. He was previously Director of Research Advocacy and Professor of Physiology and Pharmacology, Cell Biology and Development and Obstetrics and Gynecology at Oregon Health and Science University and Senior Scientist of the Oregon National Primate Research Center (ONPRC). He served for twelve years as Special Assistant to the President and Associate Director of the ONPRC. After receiving a B.S. degree and teaching certification from the University of Michigan (1971), a M.S. from North Carolina State University (1973), and a Ph.D. degree from Baylor College of Medicine (1976), Conn did a fellowship at the NIH, then joined the faculty in the Department of Pharmacology, Duke University Medical Center where he was promoted to Associate Professor in 1982. In 1984, he became Professor and Head of Pharmacology at the University of Iowa College of Medicine, a position he held for eleven years. Conn is known for his research in the area of the cellular and molecular basis of action of gonadotropin releasing hormone action in the pituitary and therapeutic approaches that restore misfolded proteins to function. His work has led to drugs that have benefitted humans and animals. Most recently, he has identified a new class of drugs, pharmacoperones, which act by regulating the intracellular trafficking of receptors, enzymes and ion channels. He has authored or co-authored over 350 publications in this area and written or edited over 200 books, including texts in neurosciences, molecular biology and endocrinology. Conn has served as the editor of many professional journals and book series (Endocrinology, Journal of Clinical Endocrinology and Metabolism, Endocrine, Methods, Progress in Molecular Biology and Translational Science and Contemporary Endocrinology). Conn served on the National Board of Medical Examiners, including two years as chairman of the reproduction and endocrinology committee. The work of his laboratory has been recognized with a MERIT award from the NIH, the J.J. Abel Award of the American Society for Pharmacology and Experimental Therapeutics, the Weitzman, Oppenheimer and Ingbar Awards of the Endocrine Society, the National Science Medal of Mexico (the Miguel Aleman Prize) and the Stevenson Award of Canada. He is the recipient of the Oregon State Award for Discovery, the Media Award of the American College of Neuropsychopharmacology and was named a distinguished Alumnus of Baylor College of Medicine in 2012. Conn is a previous member of Council for the American Society for Cell Biology and the Endocrine Society and is a prior President of the Endocrine Society, during which time he founded the Hormone Foundation and worked with political leadership to heighten the public’s awareness of diabetes. Conn’s students and fellows have gone on to become leaders in industry and academia. He is an elected member of the Mexican Institute of Medicine and a fellow of the American Association for the Advancement of Science. He is the co-author of The Animal Research War (2008) and many articles for the public and academic community on the value of animal research and the dangers posed by animal extremism. His op/eds have appeared in The Washington Post, The LA Times, The Wall Street Journal, the Des Moines Register, and elsewhere. Conn consults with organizations that are influenced by animal extremism and with universities and companies facing challenges from these groups.

Affiliations and Expertise

Texas Tech University Health Sciences Center, Lubbock, USA

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