Recent Advances in Steroid Biochemistry - 1st Edition - ISBN: 9780080197098, 9781483140148

Recent Advances in Steroid Biochemistry

1st Edition

Editors: J. R. Pasqualini
eBook ISBN: 9781483140148
Imprint: Pergamon
Published Date: 27th October 1975
Page Count: 302
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Description

Recent Advances in Steroid Biochemistry contains the proceedings of the Second International Symposium of the Journal of Steroid Biochemistry. Separating 38 papers as chapters, this book discusses the partial purification of steroid-receptor complexes; enzymatic techniques in steroid assay; mass fragmentography of steroid hormones; biological consequences of 18-hydroxylation; and steroidogenesis in adrenal cells.
This text also explores the regulation of steroidogenesis in testis; androgen binding proteins in different testis compartments; nuclear acceptor sites for glucocorticoid receptors; estrogen receptors in the pancreas; and molecular mechanisms of steroid hormone action.

Table of Contents


Front Cover

Recent Advances in Steroid Biochemistry

Copyright Page

Chapter 1. Partial Purification of Steroid-Receptor Complexes by DNA-Cellulose Chromatography and Isoelectric Focusing

Introduction

Experimental

Results and Discussion

References

Chapter 2. Enzymatic Techniques in Steroid Assay

Introduction

Experimental

Method

Results

Discussion

References

Chapter 3. Mass Fragmentography of Steroid Hormones

Introduction

Experimental

Results and Discussion

Conclusions

References

Discussion

Chapter 4. Separation and Computerized Gas Chromatography-Mass Spectrometry of Unconjugated Neutral Steroids in Plasma

Introduction

Experimental

Results

Discussion

References

Chapter 5. Steroids as Haptens: Optimal Design of Antigens for the Formation of Antibodies to Steroid Hormones

Definition of the Problem and Rationale of Approach

General Methods

Properties of Antisera to Gonadal Steroids in Relation to Structure of the Antigenic Conjugate

Concluding Remarks

References

Chapter 6. Some Aspects of the Use of 125i-Labelled Ligands for Steroid Radioimmunoassay

Introduction

Experimental

Results

Discussion

References

Chapter 7. Testosterone Binding Capacity in Relation to the Production and Metabolism of Testosterone in Dogs, Experiences of A New Method

Introduction

Materials and Methods

Results

Discussion

References

Chapter 8. Simultaneous Estimation of Testosterone, Progesterone and Androstenedione by Gas Chromatography-Mass Spectrometry with A Single Ion Detection Correlation with Radioimmunoassay

Introduction

Discussion

References

General Discussion

Chapter 9. The Preparation and use of Purified and Unpurified Dispersed Adrenal Cells and a Study of the Relationship of Their Camp and Steroid Output

Introduction

Experimental and Results

Discussion

Appendix

References

Chapter 10. Biological Consequences of 18-Hydroxylation

Introduction

Experimental

Results

Discussion

References

Chapter 11. Multifactorial Regulation of the Final Steps of Aldosterone Biosynthesis in the Rat

Stimulators of An Early Biosynthetic Step

Variable Steroidogenic Response to Stimulators

Alterations in the Activity of Enzymes Involved in the Final Steps of Aldosterone Biosynthesis

Renin-Angiotensin

Potassium

Serotonin

Pituitary Gland and ACTH

Conclusions

References

Discussion

Chapter 12. Some Comparative Studies in Adrenocortical Steroidogenesis: An Interpretation of the Functional Homologies of the Mammalian and Non-Mammalian Adrenal Cortex

Introduction

Materials and Methods

Results

Discussion

References

Chapter 13. Steroidogenesis in Adrenal Cells

Introduction

Results and Discussion

References

Discussion

Chapter 14. Stimulation Factors for Cholesterol Side-Chain Cleavage in the Corpus Luteum and Adrenal Gland

Introduction

Methods

Results

Discussion

References

Chapter 15. In Vitro Study On Corticosterone Cytochrome P-450 Binding in Relation to the Regulation of Corticosterone Metabolism

Introduction

Materials and Methods

Results

Discussion

References

General Discussion

Chapter 16. Superfusion of Rat Testes With Mixtures of Labeled Testosterone and Androstenedione

Introduction

Experimental

Results

Discussion

References

Chapter 17. Regulation of Steroidogenesis in Testis

Introduction

Experimental

Results

Discussion

References

Chapter 18. Androgen Binding Proteins in Different Testis Compartments

Preparation of Samples

Sertoli Cell Production of ABP

Regulation of ABP Production

Androgens and Spermatogenesis

References

Chapter 19. Sexual Differentiation of Hepatic Steroid Metabolism in the Rat

Introduction

Experimental

Results and Discussion

References

Discussion

Chapter 20. Relation of Structure and Biological Action of Gonadotrophins

Introduction

References

Discussion

Chapter 21. ACTH and Sulfatase Activity

Introduction

References

Steroid Nomenclature

Results

Discussion

References

Chapter 22. New Results on Oestrogen Glucuronyltransferase

Introduction

Conclusions

References

General Discussion

Chapter 23. Effects of Estrogens on Uterine Poly (A)-Rich RNA

Introduction

Experimental

Results

Discussion

References

Chapter 24. In Vitro Stimulation of Nucleolar and Nucleoplasmic RNA Polymerases in Calf Uterus

Introduction

Materials and Methods

Results

References

Discussion

Chapter 25. Hormonal Regulation of Uterine Contractility: the Role of the Adenylate Cyclase System

Introduction

Materials and Methods

Results

Discussion

References

Chapter 26. A Specific Testosterone Receptor in the Cytosol of Rat Anterior Hypophysis

Introduction

Materials and Methods

Results

Discussion

References

Chapter 27. Interaction of Aldosterone and Corticosterone with Cell Nuclei of Target Organs Under Different Functional States

Introduction

Experimental

Results

Discussion

References

Chapter 28. Interactions of ACTH With Its Adrenal Receptors: Specific Binding of Acth1–24, Its O-Nitrophenyl Sulfenyl Derivative and ACTH

Experimental Procedure

Discussion

References

Chapter 29. Nuclear Acceptor Sites for Glucocorticoid Receptors

Introduction

Results and Discussion

References

Discussion

Chapter 30. Testosterone Metabolism: A Necessary Step for Activity?

Introduction

Materials and Methods

Results

Discussion

References

Chapter 31. Investigations of Androgen-Prostatic Tissue Relationships in Superfusion Experiments Using Steroid Concentrations Approaching Those Found In Vivo

References

Discussion

Chapter 32. High Affinity Binding of Oestradiol by Rat Testis Interstitial Tissue and by Several Other Tissues of the Male Rat

Introduction

Methods

Results

Discussion

References

Chapter 33. Interaction Between Oestrogens and Neurotransmitters at the Hypophysial-Hypothalamic Level

Introduction

Experimental

Results

Discussion

References

Chapter 34. Estrogen Receptors in the Pancreas

Introduction

Experimental

Results

Discussion

References

Chapter 35. Steroid Hormone Receptors in Fetal Guinea-Pig Kidney

Introduction

Experimental

Results

Discussion

References

Chapter 36. Hormone-Induced Transformation of Receptor Proteins

Discussion

Chapter 37. Molecular Mechanisms of Steroid Hormone Action

Introduction

Results

Discussion

References

Chapter 38. Influence of Ouabain on Sodium Transport by Aldosterone-Stimulated Amphibian Epithelia

Introduction

References

General Discussion

Publisher's Announcement


Details

No. of pages:
302
Language:
English
Copyright:
© Pergamon 1975
Published:
Imprint:
Pergamon
eBook ISBN:
9781483140148

About the Editor

J. R. Pasqualini

Affiliations and Expertise

CNRS Steroid Hormone Research Unit, Foundation for Hormone Research, 26 Boulevard Brune, 75014 Paris, France

Ratings and Reviews