Recent Advances in Electron Cryomicroscopy, Part A, Volume 81

1. From envelopes to atoms: the remarkable progress of biological electron microscopy

R. Anthony Crowther

2. Single-particle applications at intermediate resolution

Bettina Böttcher and Katharina Hipp

3. Visualizing molecular machines in action: single particle analysis with structural variability

Sjors H.W. Scheres

4. Cellular Tomography

Andreas Hoenger and Cédric Bouchet-Marquis

Structural genomics is the systematic determination of 3-D structures of proteins representative of the range of protein structure and function found in nature. The goal is to build a body of structural information that will predict the structure and potential function for almost any protein from knowledge of its coding sequence. This is essential information for understanding the functioning of the human proteome, the ensemble of tens of thousands of proteins specified by the human genome.

While most structural biologists pursue structures of individual proteins or protein groups, specialists in structural genomics pursue structures of proteins on a genome wide scale. This implies large-scale cloning, expression and purification. One main advantage of this approach is economy of scale.

• Examines the three dimensional structure of all proteins of a given organism, by experimental methods such as X-ray crystallography and NMR spectroscopy
• Looks at structural genomics as a foundation of drug discovery as discovering new medicines is becoming more challenging and the pharmaceutical industry is looking to new technologies to help in this mission

Biochemists, biophysicists, cell biologists, protein chemists, structural geneticists, and structural biologists