Recent Advances in Cytometry, Part B

Recent Advances in Cytometry, Part B

Advances in Applications

5th Edition - June 1, 2011
  • Editors: Zbigniew Darzynkiewicz, Elena Holden, William Telford, Donald Wlodkowic
  • eBook ISBN: 9780123854940
  • Hardcover ISBN: 9780123854933

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Description

Cytometry is one of the most rapidly growing methodologies available for basic cell and molecular biology, cytogenetics, immunology, oncology, environmental sciences and also various fields of clinical medicine. This new edition, split into 2 Parts, is an almost completely new book, with nearly all of the chapters devoted to new topics. Like the previous volumes on cytometry published as part of the Methods in Cell Biology series, it provides a comprehensive description of particular cytometric methods and reviews their applications. Chapters present the theoretical foundations of the described methods, their applicability in experimental laboratory and clinical settings, and describes common traps and pitfalls such as problems with data interpretation, comparison with alternative assays, and choosing the optimal assay.

Key Features

  • Comprehensive presentation of cytometric methods covering theoretical applications, applicability, potential pitfalls, and comparisions to alternative assays
  • Discusses many new assays developed since the previous edition
  • Presents recent developments in cytometric intrumentation/technology

Readership

Researchers in the fields of cell and molecular biology, immunology, and cytogenetics.

Table of Contents

  • Recent Advances in Cytometry Applications: Preclinical, Clinical, and Cell Biology

    I. Preclinical and Clinical Applications

    II. Cell Biology and Cell Transplantation Therapy

    Acknowledgment

    Detection of Hematopoietic Stem Cells by Flow Cytometry

    I. Introduction

    II. The side population is highly enriched in hematopoietic stem cells

    III. Surface markers for LT-HSC purification

    Identification of Very Small Embryonic/Epiblast-Like Stem Cells (VSELs) Circulating in Peripheral Blood During Organ/Tissue Injuries

    I. Introduction

    II. Background

    III. Materials

    IV. Methods

    V. Results

    VI. Critical Aspects of the Methodology

    VII. Applications

    VIII. Future Directions

    Acknowledgments

    Apoptosis and Beyond: Cytometry in Studies of Programmed Cell Death

    I. Introduction

    II. The Biology of Apoptosis

    III. Cytometry in Cell Necrobiology

    IV. Cytometric Methods to Detect Apoptosis

    V. Time-Window in Measuring Incidence of Apoptosis

    VI. Multiparameter Detection of Apoptosis: Choosing the Right Method

    VII. Beyond Apoptosis – Analysis of Alternative Cell Death Modes

    VIII. Future Outlook

    Acknowledgements

    Assessment of Oxidative Stress-Induced DNA Damage by Immunoflourescent Analysis of 8-OxodG

    I. Redox Regulation of Cell Fate Signaling

    II. 8-OxodG as a Marker of Oxidative DNA Damage

    III. Detection of Oxidative DNA Damage Involving 8-OxodG

    IV. Concluding Remarks

    Analysis of Individual Molecular Events of DNA Damage Response by Flow- and Image-Assisted Cytometry

    I. Introduction

    II. Events of the DDR

    III. Detection of DDR Events by Cytometry

    IV. Application of Cytometry to Detect DDR Induced by Different Genotoxic Agents

    V. Interpretation of Cytometric Data: Role of Image-Assisted Cytometry

    VI. Role of Microfluidic Lab-on-a-Chip Platforms for DDR Analysis

    Fluorescence-Based Detection and Quantification of Features of Cellular Senescence

    I. Introduction

    II. Features Associated with Loss of Reproductive Cell Capability

    III. Cellular Hypertrophy

    IV. Changes Associated with Lysosomes

    V. Changes Associated with Mitochondria

    VI. Changes in the Level of Reactive Oxygen Species (ROS)

    VII. Changes Associated with Nucleus and Chromosomes

    VIII. Use of Flow Cytometry for Analysis of Cellular Senescence

    IX. Conclusion

    Acknowledgments

    Measurement of Telomere Length Using PNA Probe by Cytometry

    I. Introduction to Telomeres

    II. Technical Background

    III. Methods

    IV. Results

    V. Critical Aspects of the Flow-FISH Methodology

    VI. Conclusion and Perspectives

    Cytometry of Intracellular Signaling: From Laboratory Bench to Clinical Application

    I. Introduction

    II. Rationale

    III. Methods

    IV. Pathway Activators

    V. Pathway Inhibitors

    VI. Materials

    VII. Discussion

    VIII. Summary

    Immunophenotypic Pattern of Myeloid Populations by Flow Cytometry Analysis

    I. Introduction

    II. Granulocytes

    III. Basophils

    IV. Eosinophils

    V. Monocytes

    VI. Erythroid Precursors

    VII. Megakaryocytes

    VIII. Acute Myeloid Leukemia (AML) with Minimal Differentiation and AML without Maturation

    IX. AML with Maturation

    X. AML with t(8;21)

    XI. Acute Promyelocytic Leukemia (APL)

    XII. Acute Myelomonocytic Leukemia (AML-M4)

    XIII. Acute Monoblastic Leukemia

    XIV. Acute Erythroid Leukemia (FAB: AML-M6)

    XV. Acute Megakaryoblastic Leukemia (FAB: AML-M7)

    XVI. Blastic Plasmacytoid Dendritic Cell Neoplasm

    XVII. Chronic Myeloid Leukemia (CML, BCR–ABL+)

    XVIII. Granulocytes/Maturing Myeloid Precursors with Dyspoietic Features Associated with Myelodysplastic Syndrome (MDS)

    XIX. Chronic Myelomonocytic Leukemia (CMML)

    Flow Cytometry-Based Pharmacodynamic Monitoring After Organ Transplantation

    I. Introduction

    II. T Cells

    III. Regulatory T Cells

    IV. Dendritic Cells

    V. Conclusions

    Clinical Cytometry and Progress in HLA Antibody Detection

    I. Introduction

    II. Cell-Based Assays

    III. Antigen-Based Assays

    IV. Current Frontiers in Transplant-Related Antibody Testing

    V. Summary

    Clinical Utility of Flow Cytometry in the Study of Erythropoiesis and Nonclonal Red Cell Disorders

    I. Introduction

    II. Normal Erythroid Development

    Immunophenotypic Characterization of Bone Marrow Mast Cells in Mastocytosis and Other Mast Cell Disorders

    I. Introduction

    1. Background

    II. Methods

    III. Results

    IV. Immunophenotypic Analysis of MC from Biological Specimens Other than BM

    V. Medical Indications

    VI. Summary

    Acknowledgments

Product details

  • No. of pages: 400
  • Language: English
  • Copyright: © Academic Press 2011
  • Published: June 1, 2011
  • Imprint: Academic Press
  • eBook ISBN: 9780123854940
  • Hardcover ISBN: 9780123854933

About the Serial Volume Editors

Zbigniew Darzynkiewicz

Affiliations and Expertise

CANCER RESEARCH INSTITUTE OF THE NEW YORK MEDICAL COLLEGE AT VALHALLA

Elena Holden

William Telford

Donald Wlodkowic