Cytometry is one of the most rapidly growing methodologies available for basic cell and molecular biology, cytogenetics, immunology, oncology, environmental sciences and also various fields of clinical medicine. This new edition, split into 2 Parts, is an almost completely new book, with nearly all of the chapters devoted to new topics. Like the previous volumes on cytometry published as part of the Methods in Cell Biology series, it provides a comprehensive description of particular cytometric methods and reviews their applications. Chapters present the theoretical foundations of the described methods, their applicability in experimental laboratory and clinical settings, and describes common traps and pitfalls such as problems with data interpretation, comparison with alternative assays, and choosing the optimal assay.

Key Features

* Comprehensive presentation of cytometric methods covering theoretical applications, applicability, potential pitfalls, and comparisions to alternative assays
* Discusses many new assays developed since the previous edition
* Presents recent developments in cytometric intrumentation/technology


Researchers in the fields of cell and molecular biology, immunology, and cytogenetics.

Table of Contents

Recent Advances in Cytometry Applications: Preclinical, Clinical, and Cell Biology

I. Preclinical and Clinical Applications

II. Cell Biology and Cell Transplantation Therapy


Detection of Hematopoietic Stem Cells by Flow Cytometry

I. Introduction

II. The side population is highly enriched in hematopoietic stem cells

III. Surface markers for LT-HSC purification

Identification of Very Small Embryonic/Epiblast-Like Stem Cells (VSELs) Circulating in Peripheral Blood During Organ/Tissue Injuries

I. Introduction

II. Background

III. Materials

IV. Methods

V. Results

VI. Critical Aspects of the Methodology

VII. Applications

VIII. Future Directions


Apoptosis and Beyond: Cytometry in Studies of Programmed Cell Death

I. Introduction

II. The Biology of Apoptosis

III. Cytometry in Cell Necrobiology

IV. Cytometric Methods to Detect Apoptosis

V. Time-Window in Measuring Incidence of Apoptosis

VI. Multiparameter Detection of Apoptosis: Choosing the Right Method

VII. Beyond Apoptosis – Analysis of Alternative Cell Death Modes

VIII. Future Outlook


Assessment of Oxidative Stress-Induced DNA Damage by Immunoflourescent Analysis of 8-OxodG

I. Redox Regulation of Cell Fate Signaling

II. 8-OxodG as a Marker of Oxidative DNA Damage

III. Detection of Oxidative DNA Damage Involving 8-OxodG

IV. Concluding Remarks

Analysis of Individual Molecular Events of DNA Damage Response by Flow- and Image-Assisted Cytometry

I. Introduction

II. Events of the DDR

III. Detection of DDR Events by Cytometry

IV. Application of Cytometry to Detect DDR Induced by Different Genotoxic Agents

V. Interpretation


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© 2011
Academic Press
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