Cytometry is one of the most rapidly growing methodologies available for basic cell and molecular biology, cytogenetics, immunology, oncology, environmental sciences and also various fields of clinical medicine. This new edition, split into 2 Parts, is an almost completely new book, with nearly all of the chapters devoted to new topics. Like the previous volumes on cytometry published as part of the Methods in Cell Biology series, it provides a comprehensive description of particular cytometric methods and reviews their applications. Chapters present the theoretical foundations of the described methods, their applicability in experimental laboratory and clinical settings, and describes common traps and pitfalls such as problems with data interpretation, comparison with alternative assays, and choosing the optimal assay.

Key Features

  • Comprehensive presentation of cytometric methods covering theoretical applications, applicability, potential pitfalls, and comparisions to alternative assays
  • Discusses many new assays developed since the previous edition
  • Presents recent developments in cytometric intrumentation/technology


Researchers and students in cell, molecular and developmental biology

Table of Contents

  1. Recent advances in Cytometry Instrumentation, Probes and Methods
  2. Anja Mittag, Arkadiusz Pierzchalski, and Attila Tárnok

    Section I. Down-sizing cytometry to "micro" dimension

  3. Droplet Microfluidics for High-throughput Analysis of Cells and Particles
  4. Michele Zagnoni and Jonathan M. Cooper

  5. Parallel Imaging Microfluidic Cytometer
  6. Daniel J. Ehrlich, Brian K. McKenna, James G. Evans, Anna C. Belkina, Gerald V. Denis, David H. Sherr and Man Ching Cheung

  7. Microfluidic Systems for Live Cell Imaging
  8. Philip Lee, Terry Gaige and Paul Hung

  9. Rise of the micro-machines: microfluidics and the future of cytometry
  10. Donald Wlodkowic and Zbigniew Darzynkiewicz

  11. Label-Free Resistive-Pulse Cytometry
  12. M. R. Chapman and L. L. Sohn

    Section II. Imaging cytometry


  13. Laser Scanning Cytometry and Its Applications: A Pioneering Technology in the Field of Quantitative Imaging Cytometry
  14. Melvin Henriksen, Bruce Miller, Judith Newmark, Yousef Al-Kofahi and Elena Holden

  15. Analytical capabilities of ImageStream cytometer
  16. Ewa K. Zuba-Surma and Mariusz Z. Ratajczak

  17. Laser scanning cytometry: capturing the immune system in situ
  18. Mairi A McGrath, Angela M Morton and Margaret M Harnett

  19. Image cytometry analysis of circulating tumor cells
  20. Lori E. Lowes, David Goodale, Michael Keeney and Alison L. Allan

  21. Pre-clinical Applications of Quantitative Imaging Cytometry to Support Drug Discovery
  22. David L Krull and Richard A Peterson

  23. Leveraging Image Cytometry for the Development of Clinically Feasible Biomarkers: Evaluation of Activated Caspase-3 in Fine Needle Aspirate Biopsies
  24. Gloria Juan, Stephen J. Zoog and John F


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© 2011
Academic Press
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