
Reactive Species Detection in Biology
From Fluorescence to Electron Paramagnetic Resonance Spectroscopy
Description
Key Features
- Reviews all current advances in radical detection
- Emphasizes chemical structures and reaction schemes fundamental to radical detection and identification
- Describes the uses, advantages, and disadvantages of various probe designs
- Examines new approaches to radical probe development
Readership
Research scientists / principal investigators in the fields of chemistry, biomedical research, nutrition/food science, public health, biology/biochemistry, biomedical engineering, and other related fields dealing with antioxidants (cosmetics, home products, fuel cell research, materials research)
Table of Contents
- Dedication
- Preface
- Chapter 1. Introduction
- References
- Chapter 2. Chemistry of Reactive Species
- Abstract
- 2.1 Introduction
- 2.2 Redox Chemistry
- 2.3 Properties of Reactive Species
- References
- Chapter 3. Reactive Species in Biological Systems
- Abstract
- 3.1 Introduction
- 3.2 Extracellular Milieu
- 3.3 Membrane-Bound Enzymes
- 3.4 Cytosolic Enzymes
- 3.5 Organelle Enzymes
- References
- Chapter 4. Fluorescence Technique
- Abstract
- 4.1 Introduction
- 4.2 Fluorescence Spectroscopy and Microscopy
- 4.3 Chemistry of Redox Detection by Fluorescence
- 4.4 Classification of Fluorescent RS Probes by Specificity
- 4.5 Considerations in Fluorescence Probe Application
- References
- Chapter 5. EPR Spin Trapping
- Abstract
- 5.1 Introduction
- 5.2 Electron Paramagnetic Resonance Spectroscopy
- 5.3 Chemistry of Spin Trapping
- 5.4 Classification of Spin Traps
- 5.5 Kinetics and Thermodynamics of Spin Trapping and ph effect
- 5.6 Kinetics and Thermodynamics of Adduct Decay
- 5.7 Biostability and Cytotoxicity of Spin Traps
- 5.8 Synthesis of Spin Traps
- 5.9 Interpretation of EPR Spectra
- 5.10 Applications of Spin Trapping
- References
- Chapter 6. UV–Vis Absorption and Chemiluminescence Techniques
- Abstract
- 6.1 Introduction
- 6.2 Superoxide Radical Probes
- 6.3 Hydroxyl Radical Probes
- 6.4 Antioxidant Capacity Assays
- 6.5 Nitric Oxide and Metabolites Probes
- 6.6 Thiols Probes
- 6.7 Peroxides Probes
- 6.8 Chemiluminescence
- References
- Chapter 7. Electrochemical, Mass Spectroscopic, Immunochemical, and Nuclear Magnetic Resonance Techniques
- Abstract
- 7.1 Introduction
- 7.2 Electrochemical Techniques
- 7.3 Mass Spectroscopy
- 7.4 Immunochemical Technique
- 7.5 Nuclear Magnetic Resonance Spectroscopy and Imaging
- References
- Index
Product details
- No. of pages: 340
- Language: English
- Copyright: © Elsevier 2016
- Published: October 23, 2016
- Imprint: Elsevier
- Hardcover ISBN: 9780124200173
- eBook ISBN: 9780124200807
About the Author
Frederick Villamena
Affiliations and Expertise
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