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Rare-earth element biochemistry: Methanol dehydrogenases and lanthanide biology - 1st Edition - ISBN: 9780128238561, 9780128238578

Rare-earth element biochemistry: Methanol dehydrogenases and lanthanide biology, Volume 650

1st Edition

Serial Volume Editor: Joseph Cotruvo
eBook ISBN: 9780128238578
Hardcover ISBN: 9780128238561
Imprint: Academic Press
Published Date: 15th April 2021
Page Count: 276
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Table of Contents

Discovery of lanthanide-dependent methylotrophy and screening methods for lanthanide-dependent methylotrophs

Tomoyuki Nakagawa

Synthetic Modeling of the Structure and Function of the Lanthanide-Dependent, MDH Cofactor

Eric Schelter

Activity assays of methanol dehydrogenases

Lena Daumann

Expression, purification and properties of enzymes involved in lanthanide-dependent alcohol oxidation: XoxF4, XoxF5, ExaF/PedH, XoxG4

Ludmila Chistoserdova

Expression, purification and testing of lanthanide-dependent enzymes in Methylorubrum extorquens AM1

Norma Cecilia Martinez-Gomez

Heterologous expression, purification, and characterization of proteins involved in lanthanide utilization

Joseph A. Cotruvo Jr.

Transposon mutagenesis for methylotrophic bacteria using Methylorubrum extorquens AM1 as a model system

Elizabeth Skovran

Metals and Methylotrophy: Global Gene Expression Studies

Marina G. Kalyuzhnaya

Mass spectrometry-based approaches to study lanthanides in the phyllosphere

Julia A. Vorholt

Cultivation techniques to study lanthanide metal interactions in the haloalkaliphilic Type I methanotroph "Methylotuvimicrobium buryatense" 5GB1C

Joseph Douglas Groom Jr.


Methods in Enzymology, Volume 650 continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Biophysical methods to study lanthanide-protein interactions, Genetically encoded sensors to study lanthanide biology, Spectrophotometric methods to determine the stability constants of lanthanide-macromolecule complexes, Lanthanide-based probes for amino acid modifications, In vitro selection and application of lanthanide-dependent DNAzymes, LRET biosensors for imaging protein interactions in living cells, Synthetic Modeling of the Structure and Function of the Lanthanide-Dependent, MDH Cofactor, EPR spectroscopy of lanthanides, Macromolecular crystallography for f-element complex characterization, and much more.

Key Features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods in Enzymology series


Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists


No. of pages:
© Academic Press 2021
15th April 2021
Academic Press
eBook ISBN:
Hardcover ISBN:

Ratings and Reviews

About the Serial Volume Editor

Joseph Cotruvo

Joseph A. Cotruvo, Jr. is Assistant Professor, Louis Martarano Career Development Professor, Department of Chemistry Pennsylvania State University, PA, USA

Affiliations and Expertise

Assistant Professor, Pennsylvania State University, PA, USA