Rare-earth element biochemistry: Methanol dehydrogenases and lanthanide biology

Rare-earth element biochemistry: Methanol dehydrogenases and lanthanide biology

1st Edition - April 15, 2021
This is the Latest Edition
  • Editor: Joseph Cotruvo
  • Hardcover ISBN: 9780128238561
  • eBook ISBN: 9780128238578

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Description

Methods in Enzymology, Volume 650 continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Biophysical methods to study lanthanide-protein interactions, Genetically encoded sensors to study lanthanide biology, Spectrophotometric methods to determine the stability constants of lanthanide-macromolecule complexes, Lanthanide-based probes for amino acid modifications, In vitro selection and application of lanthanide-dependent DNAzymes, LRET biosensors for imaging protein interactions in living cells, Synthetic Modeling of the Structure and Function of the Lanthanide-Dependent, MDH Cofactor, EPR spectroscopy of lanthanides, Macromolecular crystallography for f-element complex characterization, and much more.

Key Features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods in Enzymology series

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Table of Contents

  • Discovery of lanthanide-dependent methylotrophy and screening methods for lanthanide-dependent methylotrophs

    Tomoyuki Nakagawa

    Synthetic Modeling of the Structure and Function of the Lanthanide-Dependent, MDH Cofactor

    Eric Schelter

    Activity assays of methanol dehydrogenases

    Lena Daumann

    Expression, purification and properties of enzymes involved in lanthanide-dependent alcohol oxidation: XoxF4, XoxF5, ExaF/PedH, XoxG4

    Ludmila Chistoserdova

    Expression, purification and testing of lanthanide-dependent enzymes in Methylorubrum extorquens AM1

    Norma Cecilia Martinez-Gomez

    Heterologous expression, purification, and characterization of proteins involved in lanthanide utilization

    Joseph A. Cotruvo Jr.

    Transposon mutagenesis for methylotrophic bacteria using Methylorubrum extorquens AM1 as a model system

    Elizabeth Skovran

    Metals and Methylotrophy: Global Gene Expression Studies

    Marina G. Kalyuzhnaya

    Mass spectrometry-based approaches to study lanthanides in the phyllosphere

    Julia A. Vorholt

    Cultivation techniques to study lanthanide metal interactions in the haloalkaliphilic Type I methanotroph "Methylotuvimicrobium buryatense" 5GB1C

    Joseph Douglas Groom Jr.

Product details

  • No. of pages: 276
  • Language: English
  • Copyright: © Academic Press 2021
  • Published: April 15, 2021
  • Imprint: Academic Press
  • Hardcover ISBN: 9780128238561
  • eBook ISBN: 9780128238578

About the Serial Volume Editor

Joseph Cotruvo

Joseph A. Cotruvo, Jr. is Assistant Professor, Louis Martarano Career Development Professor, Department of Chemistry Pennsylvania State University, PA, USA

Affiliations and Expertise

Assistant Professor, Pennsylvania State University, PA, USA