Rapid Virus Diagnosis - 2nd Edition - ISBN: 9780407384415, 9781483192178

Rapid Virus Diagnosis

2nd Edition

Application of Immunofluorescence

Authors: P. S. Gardner J. McQuillin
eBook ISBN: 9781483192178
Imprint: Butterworth-Heinemann
Published Date: 1st January 1980
Page Count: 332
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Rapid Virus Diagnosis: Application of Immunofluorescence presents developments in immunofluorescence as a technique for the diagnosis of virus infections. It discusses the effective and rapid methods for the diagnosis of virus infections. It addresses the application of fluorescent antibody techniques to the diagnosis and the investigation of virus infections and the assessment of their value both to the clinician and to the virologist. Some of the topics covered in the book are the fluorescence microscopy; nature of fluorescence; filter systems; transmitted light microscopy; interference filters for transmitted light microscopy; lamp centration and alignment of microscope; contrast-fluorescence condensers; photomicrography; and choice of microscope. The culture of viruses for production of antiserum is fully covered. The selection of cell lines or tissues for virus culture is discussed in detail. The text describes in depth the fluorescent antibody staining techniques. The preparation of specimens from respiratory infections is presented completely. A chapter is devoted to the respiratory syncytial virus. Another section focuses on the analysis influenza virus, paramyxoviruses, and picornaviruses. The book can provide useful information to doctors, virologists, students, and researchers.

Table of Contents


1. Introduction

2. Fluorescence Microscopy

Nature of Fluorescence

Light Sources

Filter Systems

Viewing Heads




Choice of Microscope

3. The Reagents

Culture of Viruses For Production of Antiserum

Production of Antiserum


4. Immunofluorescence Techniques, Control of Specificity and Nonspecific Fluorescence

Fluorescent Antibody Staining Techniques

Control of Specificity of Fluorescent Antibody Staining Techniques


Non-Specific Fluorescence

Absorption Techniques

5. Preparation of Specimens

Preparation of Specimens From Respiratory Infections

Preparation of Specimens From Infections Not Involving Respiratory Tract

Preparation of Tissue Culture Cells For Fluorescent Antibody Investigations

6. Respiratory Syncytial Virus

7. Influenza Viruses

8. Paramyxoviruses

Parainfluenza Viruses

Mumps Virus

9. Measles (Morbilli)

10. Picornaviruses

11. Rabies

Technical Considerations

12. Variola

13. Adenoviruses

Immunofluorescence and Tissue Culture

Direct Examination of Specimen by Immunoflourescence For Diagnosis of Adenovirus Infection

The Diagnosis of Adenovirus Infections of the Eye

14. Arboviruses (Including Togaviruses, Arenaviruses, Colorado Tick Fever)

15. Rubella

16. Herpesvirus Hominis

Investigation of Skin Lesions by Immunofluorescence

Investigation of Infections of the Conjunctivae For HVH by Immunofluorescence

Use of Immunofluorescence For Identifying HVH in Tissue Culture

Immunofluorescence and Antiviral Treatment of HVH Eye Infections

HVH and Encephalitis

Disseminated HVH Infection of the Newborn

Types of HVH

17. Miscellaneous Viruses in the Herpesvirus Group

Herpes Varicellae-Herpes Zoster

Cytomegalovirus of Man

Epstein-Barr Virus

18. Detection of Virus-Specific IgM by Immunofluorescence

Methods For Demonstration of Virus-Specific IgM Activity

Sources of Error



Herpesvirus Hominis

Epstein-Barr Virus


Influenza A



19. Application of Immunofluorescence Technique To Clinical Virology

Management of Patients and Rational Use of Antibiotics

The Use of Antiviral Drugs

Virus Infection Outside Acute Stage of Illness

The Use of Rapid Diagnostic Techniques in Fatal Infections

Virus Infection in the Immunosuppressed Patient And Its Management

Frequently Occurring Viruses Producing Unusual Symptoms

Viruses Difficult To Cultivate in the Laboratory

Prevention of Ward Cross-Infection With Viruses

Virus Diagnosis in Areas Distant From A Virus Laboratory

Rapid Diagnosis in Epidemiology

Immunofluorescence As A Laboratory Aid



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© Butterworth-Heinemann 1980
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About the Author

P. S. Gardner

J. McQuillin

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