Rapid Virus Diagnosis

Rapid Virus Diagnosis

Application of Immunofluorescence

2nd Edition - January 1, 1980

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  • Authors: P. S. Gardner, J. McQuillin
  • eBook ISBN: 9781483192178

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Description

Rapid Virus Diagnosis: Application of Immunofluorescence presents developments in immunofluorescence as a technique for the diagnosis of virus infections. It discusses the effective and rapid methods for the diagnosis of virus infections. It addresses the application of fluorescent antibody techniques to the diagnosis and the investigation of virus infections and the assessment of their value both to the clinician and to the virologist. Some of the topics covered in the book are the fluorescence microscopy; nature of fluorescence; filter systems; transmitted light microscopy; interference filters for transmitted light microscopy; lamp centration and alignment of microscope; contrast-fluorescence condensers; photomicrography; and choice of microscope. The culture of viruses for production of antiserum is fully covered. The selection of cell lines or tissues for virus culture is discussed in detail. The text describes in depth the fluorescent antibody staining techniques. The preparation of specimens from respiratory infections is presented completely. A chapter is devoted to the respiratory syncytial virus. Another section focuses on the analysis influenza virus, paramyxoviruses, and picornaviruses. The book can provide useful information to doctors, virologists, students, and researchers.

Table of Contents


  • Contents

    1. Introduction

    2. Fluorescence Microscopy

    Nature of Fluorescence

    Light Sources

    Filter Systems

    Viewing Heads

    Objectives

    Oculars

    Photomicrography

    Choice of Microscope

    3. The Reagents

    Culture of Viruses For Production of Antiserum

    Production of Antiserum

    Conjugates

    4. Immunofluorescence Techniques, Control of Specificity and Nonspecific Fluorescence

    Fluorescent Antibody Staining Techniques

    Control of Specificity of Fluorescent Antibody Staining Techniques

    Mountants

    Non-Specific Fluorescence

    Absorption Techniques

    5. Preparation of Specimens

    Preparation of Specimens From Respiratory Infections

    Preparation of Specimens From Infections Not Involving Respiratory Tract

    Preparation of Tissue Culture Cells For Fluorescent Antibody Investigations

    6. Respiratory Syncytial Virus

    7. Influenza Viruses

    8. Paramyxoviruses

    Parainfluenza Viruses

    Mumps Virus

    9. Measles (Morbilli)

    10. Picornaviruses

    11. Rabies

    Technical Considerations

    12. Variola

    13. Adenoviruses

    Immunofluorescence and Tissue Culture

    Direct Examination of Specimen by Immunoflourescence For Diagnosis of Adenovirus Infection

    The Diagnosis of Adenovirus Infections of the Eye

    14. Arboviruses (Including Togaviruses, Arenaviruses, Colorado Tick Fever)

    15. Rubella

    16. Herpesvirus Hominis

    Investigation of Skin Lesions by Immunofluorescence

    Investigation of Infections of the Conjunctivae For HVH by Immunofluorescence

    Use of Immunofluorescence For Identifying HVH in Tissue Culture

    Immunofluorescence and Antiviral Treatment of HVH Eye Infections

    HVH and Encephalitis

    Disseminated HVH Infection of the Newborn

    Types of HVH

    17. Miscellaneous Viruses in the Herpesvirus Group

    Herpes Varicellae-Herpes Zoster

    Cytomegalovirus of Man

    Epstein-Barr Virus

    18. Detection of Virus-Specific IgM by Immunofluorescence

    Methods For Demonstration of Virus-Specific IgM Activity

    Sources of Error

    Cytomegalovirus

    Rubella

    Herpesvirus Hominis

    Epstein-Barr Virus

    Mumps

    Influenza A

    Rotavirus

    Measles

    19. Application of Immunofluorescence Technique To Clinical Virology

    Management of Patients and Rational Use of Antibiotics

    The Use of Antiviral Drugs

    Virus Infection Outside Acute Stage of Illness

    The Use of Rapid Diagnostic Techniques in Fatal Infections

    Virus Infection in the Immunosuppressed Patient And Its Management

    Frequently Occurring Viruses Producing Unusual Symptoms

    Viruses Difficult To Cultivate in the Laboratory

    Prevention of Ward Cross-Infection With Viruses

    Virus Diagnosis in Areas Distant From A Virus Laboratory

    Rapid Diagnosis in Epidemiology

    Immunofluorescence As A Laboratory Aid

    Index


Product details

  • No. of pages: 332
  • Language: English
  • Copyright: © Butterworth-Heinemann 1980
  • Published: January 1, 1980
  • Imprint: Butterworth-Heinemann
  • eBook ISBN: 9781483192178

About the Authors

P. S. Gardner

J. McQuillin

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