Rapid Detection and Identification of Infectious Agents

Rapid Detection and Identification of Infectious Agents

1st Edition - January 28, 1985

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  • Editor: David Kingsbury
  • eBook ISBN: 9780323156578

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Description

Rapid Detection and Identification of Infectious Agents is a collection of papers presented at the International Symposium on Rapid Detection and Identification of Infectious Agents held on October 5-7, 1983, in Oakland, California, and organized by the Naval Biosciences Laboratory of the School of Public Health of the University of California at Berkeley. Contributors examine progress in the field of rapid diagnosis of infectious diseases, with a particular emphasis on DNA probe-based assays and monoclonal and polyclonal antibody-based immunoassays. This volume is organized into five sections encompassing 20 chapters. It begins with an overview of state-of-the-art methods for rapid detection and identification of infectious agents, including technology that is currently applied in clinical microbiology, as well as concerns regarding the political and scientific climates, which have an impact on health care and clinical microbiology. Chapters are organized to deal with a single diagnostic type of test for a given broad group of organisms. The approach is to compare the strengths and weaknesses of each of the new diagnostic procedures, using the same type of clinical material whenever possible. The book gives consideration to the fundamental design of DNA probes and probe assay systems, the clinical comparison of immunologic assays for the diagnosis of meningococcal disease, and immunodiagnostics for viral and parasitic pathogens. This book will be of value to scientists and researchers interested in immunology and infectious diseases, as well as the methods used to detect and identify them.

Table of Contents


  • Contributors

    Preface

    Immunodiagnostics: Viral and Parasitic Pathogens

    Technological Advances in the Clinical Microbiology Laboratory: Sensitivity, Specificity, and Cost Effectiveness

    Enzymatic Assays for the Diagnosis of Infectious Diseases

    Viral Hepatitis-A Model for Rapid Diagnosis of Infectious Disease

    Serodiagnosis of Herpes Simplex Virus and Cytomegalovirus Infection with Monoclonal Antibodies

    Recent Developments on Diagnostic Methods for Toxoplasmosis and Chagas' Disease

    Molecular Probes: Viral, Subviral, and Parasitic Pathogens

    Defined Viral Probes for the Detection of HSV, CMV, and HPV

    Aspects of Using Nucleic Acid Filter Hybridization to Characterize and Detect Enteroviral RNAs

    Rapid Identification of Leishmania Species Using Specific Hybridization of Kinetoplast DNA Sequences

    Detection of Viroids in Plants

    Immunodiagnostics: Bacterial and Fungal Pathogens

    Sensitive Homogeneous Enzyme Immunoassays for Microbial Antigens

    Latex Agglutination Tests for the Rapid Diagnosis of Infectious Disease

    Comparison of Immunoassays for Detection of Bacterial Antigens in Cerebrospinal Fluid from Meningitis Patients

    Detection of Coccidioides immitis Infection by Enzyme Immunoassay

    Molecular Probes: Bacterial and Fungal Pathogens

    Selection of DNA Probes for Use in the Diagnosis of Infectious Disease

    Rapid Detection of Mycoplasmas with DNA Probes

    Synthesis and Detection of 3'-OH Terminal Biotin-Labeled DNA Probes

    Chemiluminescent and Fluorescent Probes for DNA Hybridization Systems

    Monoclonal Antibody Specific for Double-Stranded DNA: A Nonradioactive Probe Method for Detection of DNA Hybridization

    Summary Presentations and Conclusions

    Summary of DNA Probes

    Immunodiagnostics in Clinical Microbiology

    Concluding Remarks

    Index




Product details

  • No. of pages: 310
  • Language: English
  • Copyright: © Academic Press 1985
  • Published: January 28, 1985
  • Imprint: Academic Press
  • eBook ISBN: 9780323156578

About the Editor

David Kingsbury

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