Rapid Detection and Identification of Infectious Agents - 1st Edition - ISBN: 9780124085503, 9780323156578

Rapid Detection and Identification of Infectious Agents

1st Edition

Editors: David Kingsbury
eBook ISBN: 9780323156578
Imprint: Academic Press
Published Date: 28th January 1985
Page Count: 310
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Description

Rapid Detection and Identification of Infectious Agents is a collection of papers presented at the International Symposium on Rapid Detection and Identification of Infectious Agents held on October 5-7, 1983, in Oakland, California, and organized by the Naval Biosciences Laboratory of the School of Public Health of the University of California at Berkeley. Contributors examine progress in the field of rapid diagnosis of infectious diseases, with a particular emphasis on DNA probe-based assays and monoclonal and polyclonal antibody-based immunoassays. This volume is organized into five sections encompassing 20 chapters. It begins with an overview of state-of-the-art methods for rapid detection and identification of infectious agents, including technology that is currently applied in clinical microbiology, as well as concerns regarding the political and scientific climates, which have an impact on health care and clinical microbiology. Chapters are organized to deal with a single diagnostic type of test for a given broad group of organisms. The approach is to compare the strengths and weaknesses of each of the new diagnostic procedures, using the same type of clinical material whenever possible. The book gives consideration to the fundamental design of DNA probes and probe assay systems, the clinical comparison of immunologic assays for the diagnosis of meningococcal disease, and immunodiagnostics for viral and parasitic pathogens. This book will be of value to scientists and researchers interested in immunology and infectious diseases, as well as the methods used to detect and identify them.

Table of Contents


Contributors

Preface

Immunodiagnostics: Viral and Parasitic Pathogens

Technological Advances in the Clinical Microbiology Laboratory: Sensitivity, Specificity, and Cost Effectiveness

Enzymatic Assays for the Diagnosis of Infectious Diseases

Viral Hepatitis-A Model for Rapid Diagnosis of Infectious Disease

Serodiagnosis of Herpes Simplex Virus and Cytomegalovirus Infection with Monoclonal Antibodies

Recent Developments on Diagnostic Methods for Toxoplasmosis and Chagas' Disease

Molecular Probes: Viral, Subviral, and Parasitic Pathogens

Defined Viral Probes for the Detection of HSV, CMV, and HPV

Aspects of Using Nucleic Acid Filter Hybridization to Characterize and Detect Enteroviral RNAs

Rapid Identification of Leishmania Species Using Specific Hybridization of Kinetoplast DNA Sequences

Detection of Viroids in Plants

Immunodiagnostics: Bacterial and Fungal Pathogens

Sensitive Homogeneous Enzyme Immunoassays for Microbial Antigens

Latex Agglutination Tests for the Rapid Diagnosis of Infectious Disease

Comparison of Immunoassays for Detection of Bacterial Antigens in Cerebrospinal Fluid from Meningitis Patients

Detection of Coccidioides immitis Infection by Enzyme Immunoassay

Molecular Probes: Bacterial and Fungal Pathogens

Selection of DNA Probes for Use in the Diagnosis of Infectious Disease

Rapid Detection of Mycoplasmas with DNA Probes

Synthesis and Detection of 3'-OH Terminal Biotin-Labeled DNA Probes

Chemiluminescent and Fluorescent Probes for DNA Hybridization Systems

Monoclonal Antibody Specific for Double-Stranded DNA: A Nonradioactive Probe Method for Detection of DNA Hybridization

Summary Presentations and Conclusions

Summary of DNA Probes

Immunodiagnostics in Clinical Microbiology

Concluding Remarks

Index




Details

No. of pages:
310
Language:
English
Copyright:
© Academic Press 1985
Published:
Imprint:
Academic Press
eBook ISBN:
9780323156578

About the Editor

David Kingsbury