Radiation Oncology and Radiotherapy Part B

1st Edition, Volume 174 - January 31, 2023
Editors: Ai Sato, Jeffrey Kraynak, Ariel E. Marciscano, Lorenzo Galluzzi
Language: English
Hardback ISBN:
9 7 8 - 0 - 3 2 3 - 8 9 9 4 7 - 5
eBook ISBN:
9 7 8 - 0 - 3 2 3 - 8 9 9 4 8 - 2

Radiation Oncology and Radiotherapy, Part B, Volume 174 in the Methods in Cell Biology series, highlights advances in the field, with this new volume presenting interesti… Read more

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Radiation Oncology and Radiotherapy, Part B, Volume 174 in the Methods in Cell Biology series, highlights advances in the field, with this new volume presenting interesting chapters on timely topics including CT-assisted focal irradiation of tumors in mice, Methods to preserve correct dosimetry in small animal irradiators, Monitoring TGFbeta signaling in irradiated tumors, Cytofluorometric characterization of the lymphoid compartment of irradiated tumors, Cytofluorometric characterization of the myeloid compartment of irradiated tumors, Mass cytometry to characterize the immune infiltrate of irradiated tumors, Characterization of the immune infiltrate in irradiated mouse tumor by multiplex immunofluorescence, and much more.

Other chapters cover Methods to study FOXP3+CD8+ cells in irradiated bone metastases, Luminex-based characterization of cytokine signaling by irradiated tumors, Explant-based assessment of anticancer immunity in irradiated tumors, Pipeline to characterize the TCR repertoire of irradiated tumors, Pipeline to identify tumor neoantigens exposed by radiation, and more.

Cover image
Title page
Table of Contents
Series page
Copyright page
Contributors
Radiation therapy: An old dog learning new tricks
References
Chapter 1: Flow cytometry-assisted quantification of immune cells infiltrating irradiated tumors in mice
Abstract
1 Introduction
2 Materials
3 Methods
4 FACS analysis and TIL quantification
5 Timing of the sampling
6 Notes
7 Concluding remarks
Disclosures
Acknowledgements
References
Chapter 2: Cytofluorometric characterization of the myeloid compartment of irradiated mouse tumors
Abstract
1 Introduction
2 Materials
3 Cell culture
4 Oral tumor implantation
5 In vivo imaging
6 Irradiation
7 Flow cytometry analysis
8 Concluding remarks
9 Notes
Disclosures
Acknowledgments
References
Chapter 3: Mass cytometry to characterize the immune lung cancer microenvironment
Abstract
1 Introduction
2 Materials
3 Methods
4 Notes
5 Concluding remarks
Acknowledgments
References
Chapter 4: Characterization of the immune infiltrate in mouse tissue by multiplex immunofluorescence
Abstract
1 Introduction
2 Multiplex immunofluorescence staining
3 Materials
4 Methods
5 Multiplex imaging
6 Conclusion
Acknowledgements
References
Chapter 5: Ex vivo analysis of radiation effects on tumor infiltrating immune cells using tumor explants
Abstract
1 Introduction
2 Materials
3 Cancer cell culture and in vivo tumor establishment
4 Tumor explant processing
5 Cell surface staining and viability determination
6 Flow cytometry: Running samples and analysis protocol
7 Notes
Acknowledgements
References
Chapter 6: Assessment of immunological memory formation in vivo
Abstract
1 Introduction
2 Material
3 Method
4 Notes
5 Concluding remarks
References
Chapter 7: MorphoMacro for in vivo and ex vivo quantitative morphometric analysis of microglia
Abstract
1 Introduction
2 Materials and methods
3 Expected results
4 Notes
5 Concluding remarks
Funding
Acknowledgments
References
Chapter 8: Quantitative assessment of mitophagy in irradiated cancer cells
Abstract
1 Introduction
2 Materials
3 Methods
4 Notes
5 Concluding remarks
Acknowledgments
Conflicts of interest
References
Chapter 9: Quantification of beta-galactosidase activity as a marker of radiation-driven cellular senescence
Abstract
1 Introduction
2 Materials
3 Methods
4 Concluding remarks
Conflict of interest statement
References
Chapter 10: A simple method to assess clonogenic survival of irradiated cancer cells
Abstract
1 Introduction
2 Materials
3 Establishment of B16-OVA cell line and radiotherapy
4 Concluding remarks
Disclosures
References
Chapter 11: Clonogenic assay to measure bystander cytotoxicity of targeted alpha-particle therapy
Abstract
1 Introduction
2 Materials
3 Cell seeding for clonogenic assay
4 Cell irradiation using 223RaCl2
5 Determination of clonogenic cell survival in donor and recipient cells
6 Concluding remarks
7 Notes
References

Ai Sato

Ai Sato is a research specialist at Stich Radiation Oncology, New York, NY, USA

Affiliations and expertise

Stich Radiation Oncology, New York, NY, USA

Jeffrey Kraynak

Jeffrey Kraynak is a research laboratory specialist at Stich Radiation Oncology, New York, NY, USA.

Affiliations and expertise

research laboratory specialist at Stich Radiation Oncology, New York, NY, USA.

Ariel E. Marciscano

Dr. Ariel E. Marciscano is an assistant professor of Radiation Oncology at Weill Cornell Medical College.

Affiliations and expertise

Assistant Professor of Radiation Oncology, Weill Cornell Medical College

Lorenzo Galluzzi

Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome “La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology. Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals: OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience.

Affiliations and expertise

Assistant Professor of Cell Biology in Radiation Oncology, Department of Radiation Oncology, Weill Cornell Medical College, NY, USA