Quantitative Analysis of Steroids

Preface1. Introduction 1.1 Problems and Tendencies in Contemporary Steroid Analysis 1.2 Literature Sources on Analysis of Steroids 1.3 Scope and Limitations References2. Sex Hormones and Related Materials 2.1 Fundamentals of the Chemistry of Sex Hormones 2.2 Methods of Analysis of Sex Hormones 2.2.1 Ultraviolet Spectrophotometric Methods Based on Natural Absorption 2.2.2 Ultraviolet Spectrophotometric and Colorimetric Methods Based on Chemical Reactions 2.2.3 Fluorimetry 2.2.4 Infrared Spectrophotometry 2.2.5 NMR Spectrometry 2.2.6 Polarography 2.2.7 Titrimetric Methods 2.2.8 Gravimetric Methods 2.2.9 Column Chromatography 2.2.10 Thin-Layer Chromatography 2.2.11 Gas Chromatography 2.2.12 High-Performance Liquid Chromatography 2.2.13 Radioimmunoassay and Other Radioisotope Methods 2.2.14 Enzymatic Methods 2.2.15 Mass Spectrometry and Related Techniques 2.2.16 Miscellaneous Methods 2.3 Problems in the Analysis of Sex Hormones 2.3.1 Purity Control of Bulk Hormones 2.3.2 Determination of Sex Hormones in Pharmaceutical Preparations 2.3.3 Determination of Sex Hormones and their Metabolites in Biological Samples 2.3.4. Determination of Sex Hormones and Anabolic Agents in Foods References3. Corticosteroids 3.1 Fundamentals of the Chemistry of Corticosteroids 3.2 Methods of Analysis of Corticosteroids 3.2.1 Ultraviolet Spectrophotometric and Colorimetric Methods Based on Chemical Reactions 3.2.2 Fluorimetry 3.2.3 Polarography 3.2.4 Column Chromatography 3.2.5 Paper and Thin-Layer Chromatography 3.2.6 Gas Chromatography 3.2.7 High-Performance Liquid Chromatography 3.2.8 Radioimmunoassay and Other Radioisotope Methods 3.2.9 Enzymatic Methods 3.2.10 Mass Spectrometry and Related Techniques 3.2.11 Miscellaneous Methods 3.3 Problems in the Analysis of Corticosteroids 3.3.1 Purity Control of Bulk Corticosteroids 3.3.2 Determination of Corticosteroids in Pharmaceutical Preparations 3.3.3 Determination of Corticosteroids and their Metabolites in Biological Samples 3.3.4 Determination of Corticosteroids in Foods References4. Cholesterol and Related Sterols 4.1 Fundamentals of the Chemistry of Sterols 4.2 Methods for Analysis of Sterols 4.2.1 Spectrophotometric and Colorimetric Methods 4.2.2 Fluorimetry 4.2.3 Digitonide Precipitation Methods 4.2.4 Electroanalytical Methods 4.2.5 Column Chromatography 4.2.6 Thin-Layer Chromatography 4.2.7 Gas Chromatography 4.2.8 High-Performance Liquid Chromatography 4.2.9 Enzymatic Methods 4.2.10 Mass Spectrometry and Related Techniques 4.2.11 Miscellaneous Methods 4.3 Problems in the Analysis of Sterols 4.3.1 Determination of Cholesterol in Blood Serum and Other Biological Samples 4.3.2 Determination of Cholesterol in the Raw Materials of its Production (Wool Wax, Spinal Cord) 4.3.3 Determination of Sterols in Plants 4.3.4 Determination of Sterols in Foods References5. Vitamins D and Related Materials 5.1 Fundamentals of the Chemistry of Vitamins D 5.2 Methods of Vitamin D Analysis 5.2.1 Ultraviolet Spectrophotometric Methods 5.2.2 Colorimetric Methods 5.2.3 Infrared Spectrophotometry 5.2.4 NMR Spectrometry 5.2.5 Polarography 5.2.6 Column Chromatography 5.2.7 Thin-Layer Chromatography 5.2.8 Gas Chromatography 5.2.9 High-Performance Liquid Chromatography 5.2.10 Competitive Protein Binding Method and Other Radioisotope Techniques 5.2.11 Mass Spectrometry and Related Techniques 5.2.12 Miscellaneous Methods 5.3 Problems in the Analysis of Vitamins D 5.3.1 Purity Control of Crystalline Vitamins D 5.3.2 Analysis of Vitamin D Resins and Other Irradiation Mixtures 5.3.3 Determination of Vitamins D in Pharmaceutical Preparations 5.3.4 Determination of Vitamins D in Foods 5.3.5 Determination of Vitamins D and their Metabolites in Blood References6. Bile Acids 6.1 Fundamentals of the Chemistry of Bile Acids 6.2 Methods of Analysis of Bile Acids 6.2.1 Colorimetric Methods 6.2.2 Fluorimetry 6.2.3 Column Chromatography 6.2.4 Thin-Layer Chromatography 6.2.5 Gas Chromatography 6.2.6 High-Performance Liquid Chromatography 6.2.7 Enzymatic Methods 6.2.8 Mass Spectrometry and Related Techniques 6.2.9 Radioimmunoassay 6.2.10 Miscellaneous Methods 6.3 Problems in the Analysis of Bile Acids 6.3.1 Determination of Bile Acids and Conjugates in Biological Samples 6.3.2 Analysis of Bulk Bile Acids and Pharmaceutical Preparations Made Therefrom References7. Cardiac Glycosides 7.1 Fundamentals of the Chemistry of Cardiac Glycosides 7.2 Methods of Analysis of Cardiac Glycosides 7.2.1 Ultraviolet Spectrophotometry 7.2.2 Colorimetric Methods 7.2.3 Fluorimetry 7.2.4 Column Chromatography 7.2.5 Thin-Layer Chromatography 7.2.6 Gas Chromatography 7.2.7 High-Performance Liquid Chromatography 7.2.8 Radioimmunoassay and Enzyme Immunoassay 7.2.9 Miscellaneous Methods 7.3 Problems in the Analysis of Cardiac Glycosides 7.3.1 Purity Control in Isolated Cardiac Glycosides 7.3.2 Determination of Cardiac Glycosides in Pharmaceutical Preparations 7.3.3 Determination of Cardiac Glycosides in Plants 7.3.4 Determination of Cardiac Glycosides and their Metabolites in Body Fluids References8. Diosgenin and Related Sapogenins 8.1 Fundamentals of the Chemistry of Sapogenins 8.2 Methods of Analysis of Sapogenins 8.2.1 Colorimetric Methods 8.2.2 Infrared Spectrophotometry 8.2.3 Thin-Layer Chromatography 8.2.4 Column and High-Performance Column Chromatography 8.2.5 Gas Chromatography 8.2.6 Miscellaneous Methods 8.3 Problems in the Analysis of Sapogenins 8.3.1 Purity Control of Bulk Sapogenins 8.3.2 Determination of Sapogenins in Plants References9. Miscellaneous Steroids 9.1 Ecdysones 9.2 Solasodine and Related Alkaloids 9.3 Pancuronium Bromide and Related Quaternary Ammonium Compounds ReferencesSubject Index