Quantitative Analysis of Steroids, Volume 5

1st Edition

Print ISBN: 9780444996985
eBook ISBN: 9780444597526
Imprint: Elsevier Science
Published Date: 1st January 1983
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Studies in Analytical Chemistry, Volume 5: Quantitative Analysis of Steroids covers the pharmaceutical aspects of the analysis of steroid hormones. This book is divided into nine chapters that examine the biological-clinical analysis of other important groups of steroids, including sterols, vitamin D, bile acids, cardiac glycosides, and sapogenins.

The material in the book is classified according to the main groups of steroids, individual chapters being devoted to sex hormones, corticosteroids, sterols, vitamins D, bile acids, cardiac glycosides, sapogenins, and miscellaneous steroids. The structure of each chapter is the same. In section 1 the fundamentals of the chemistry of the group of steroids in question are outlined. Section 2 provides a detailed description of the use of various spectroscopic, chromatographic, protein-binding and other methods, while Section 3 deals with the main problems encountered in the analysis of the group of steroids in question and with their solution using the methods described in Section 2.

This book will prove useful to steroid and analytical chemists.

Table of Contents


1. Introduction

1.1 Problems and Tendencies in Contemporary Steroid Analysis

1.2 Literature Sources on Analysis of Steroids

1.3 Scope and Limitations


2. Sex Hormones and Related Materials

2.1 Fundamentals of the Chemistry of Sex Hormones

2.2 Methods of Analysis of Sex Hormones

2.2.1 Ultraviolet Spectrophotometric Methods Based on Natural Absorption

2.2.2 Ultraviolet Spectrophotometric and Colorimetric Methods Based on Chemical Reactions

2.2.3 Fluorimetry

2.2.4 Infrared Spectrophotometry

2.2.5 NMR Spectrometry

2.2.6 Polarography

2.2.7 Titrimetric Methods

2.2.8 Gravimetric Methods

2.2.9 Column Chromatography

2.2.10 Thin-Layer Chromatography

2.2.11 Gas Chromatography

2.2.12 High-Performance Liquid Chromatography

2.2.13 Radioimmunoassay and Other Radioisotope Methods

2.2.14 Enzymatic Methods

2.2.15 Mass Spectrometry and Related Techniques

2.2.16 Miscellaneous Methods

2.3 Problems in the Analysis of Sex Hormones

2.3.1 Purity Control of Bulk Hormones

2.3.2 Determination of Sex Hormones in Pharmaceutical Preparations

2.3.3 Determination of Sex Hormones and their Metabolites in Biological Samples

2.3.4. Determination of Sex Hormones and Anabolic Agents in Foods


3. Corticosteroids

3.1 Fundamentals of the Chemistry of Corticosteroids

3.2 Methods of Analysis of Corticosteroids

3.2.1 Ultraviolet Spectrophotometric and Colorimetric Methods Based on Chemical Reactions



© Elsevier Science 1983
Elsevier Science
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@qu:This is a very comprehensive and well-written text. The variety of methods for the analysis and quantitation of several main groups of steroids... are covered extremely well. This book will be of great use to chemists who need to design, understand, and explore the quantitation of the families of steroids covered. @source: Analytical Chemistry