Quantitative Analysis of Steroids - 1st Edition - ISBN: 9780444996985, 9780444597526

Quantitative Analysis of Steroids, Volume 5

1st Edition

Authors: S. Görög
eBook ISBN: 9780444597526
Imprint: Elsevier Science
Published Date: 1st January 1983
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Table of Contents


Preface

1. Introduction

1.1 Problems and Tendencies in Contemporary Steroid Analysis

1.2 Literature Sources on Analysis of Steroids

1.3 Scope and Limitations

References

2. Sex Hormones and Related Materials

2.1 Fundamentals of the Chemistry of Sex Hormones

2.2 Methods of Analysis of Sex Hormones

2.2.1 Ultraviolet Spectrophotometric Methods Based on Natural Absorption

2.2.2 Ultraviolet Spectrophotometric and Colorimetric Methods Based on Chemical Reactions

2.2.3 Fluorimetry

2.2.4 Infrared Spectrophotometry

2.2.5 NMR Spectrometry

2.2.6 Polarography

2.2.7 Titrimetric Methods

2.2.8 Gravimetric Methods

2.2.9 Column Chromatography

2.2.10 Thin-Layer Chromatography

2.2.11 Gas Chromatography

2.2.12 High-Performance Liquid Chromatography

2.2.13 Radioimmunoassay and Other Radioisotope Methods

2.2.14 Enzymatic Methods

2.2.15 Mass Spectrometry and Related Techniques

2.2.16 Miscellaneous Methods

2.3 Problems in the Analysis of Sex Hormones

2.3.1 Purity Control of Bulk Hormones

2.3.2 Determination of Sex Hormones in Pharmaceutical Preparations

2.3.3 Determination of Sex Hormones and their Metabolites in Biological Samples

2.3.4. Determination of Sex Hormones and Anabolic Agents in Foods

References

3. Corticosteroids

3.1 Fundamentals of the Chemistry of Corticosteroids

3.2 Methods of Analysis of Corticosteroids

3.2.1 Ultraviolet Spectrophotometric and Colorimetric Methods Based on Chemical Reactions

3.2.2 Fluorimetry

3.2.3 Polarography

3.2.4 Column Chromatography

3.2.5 Paper and Thin-Layer Chromatography

3.2.6 Gas Chromatography

3.2.7 High-Performance Liquid Chromatography

3.2.8 Radioimmunoassay and Other Radioisotope Methods

3.2.9 Enzymatic Methods

3.2.10 Mass Spectrometry and Related Techniques

3.2.11 Miscellaneous Methods

3.3 Problems in the Analysis of Corticosteroids

3.3.1 Purity Control of Bulk Corticosteroids

3.3.2 Determination of Corticosteroids in Pharmaceutical Preparations

3.3.3 Determination of Corticosteroids and their Metabolites in Biological Samples

3.3.4 Determination of Corticosteroids in Foods

References

4. Cholesterol and Related Sterols

4.1 Fundamentals of the Chemistry of Sterols

4.2 Methods for Analysis of Sterols

4.2.1 Spectrophotometric and Colorimetric Methods

4.2.2 Fluorimetry

4.2.3 Digitonide Precipitation Methods

4.2.4 Electroanalytical Methods

4.2.5 Column Chromatography

4.2.6 Thin-Layer Chromatography

4.2.7 Gas Chromatography

4.2.8 High-Performance Liquid Chromatography

4.2.9 Enzymatic Methods

4.2.10 Mass Spectrometry and Related Techniques

4.2.11 Miscellaneous Methods

4.3 Problems in the Analysis of Sterols

4.3.1 Determination of Cholesterol in Blood Serum and Other Biological Samples

4.3.2 Determination of Cholesterol in the Raw Materials of its Production (Wool Wax, Spinal Cord)

4.3.3 Determination of Sterols in Plants

4.3.4 Determination of Sterols in Foods

References

5. Vitamins D and Related Materials

5.1 Fundamentals of the Chemistry of Vitamins D

5.2 Methods of Vitamin D Analysis

5.2.1 Ultraviolet Spectrophotometric Methods

5.2.2 Colorimetric Methods

5.2.3 Infrared Spectrophotometry

5.2.4 NMR Spectrometry

5.2.5 Polarography

5.2.6 Column Chromatography

5.2.7 Thin-Layer Chromatography

5.2.8 Gas Chromatography

5.2.9 High-Performance Liquid Chromatography

5.2.10 Competitive Protein Binding Method and Other Radioisotope Techniques

5.2.11 Mass Spectrometry and Related Techniques

5.2.12 Miscellaneous Methods

5.3 Problems in the Analysis of Vitamins D

5.3.1 Purity Control of Crystalline Vitamins D

5.3.2 Analysis of Vitamin D Resins and Other Irradiation Mixtures

5.3.3 Determination of Vitamins D in Pharmaceutical Preparations

5.3.4 Determination of Vitamins D in Foods

5.3.5 Determination of Vitamins D and their Metabolites in Blood

References

6. Bile Acids

6.1 Fundamentals of the Chemistry of Bile Acids

6.2 Methods of Analysis of Bile Acids

6.2.1 Colorimetric Methods

6.2.2 Fluorimetry

6.2.3 Column Chromatography

6.2.4 Thin-Layer Chromatography

6.2.5 Gas Chromatography

6.2.6 High-Performance Liquid Chromatography

6.2.7 Enzymatic Methods

6.2.8 Mass Spectrometry and Related Techniques

6.2.9 Radioimmunoassay

6.2.10 Miscellaneous Methods

6.3 Problems in the Analysis of Bile Acids

6.3.1 Determination of Bile Acids and Conjugates in Biological Samples

6.3.2 Analysis of Bulk Bile Acids and Pharmaceutical Preparations Made Therefrom

References

7. Cardiac Glycosides

7.1 Fundamentals of the Chemistry of Cardiac Glycosides

7.2 Methods of Analysis of Cardiac Glycosides

7.2.1 Ultraviolet Spectrophotometry

7.2.2 Colorimetric Methods

7.2.3 Fluorimetry

7.2.4 Column Chromatography

7.2.5 Thin-Layer Chromatography

7.2.6 Gas Chromatography

7.2.7 High-Performance Liquid Chromatography

7.2.8 Radioimmunoassay and Enzyme Immunoassay

7.2.9 Miscellaneous Methods

7.3 Problems in the Analysis of Cardiac Glycosides

7.3.1 Purity Control in Isolated Cardiac Glycosides

7.3.2 Determination of Cardiac Glycosides in Pharmaceutical Preparations

7.3.3 Determination of Cardiac Glycosides in Plants

7.3.4 Determination of Cardiac Glycosides and their Metabolites in Body Fluids

References

8. Diosgenin and Related Sapogenins

8.1 Fundamentals of the Chemistry of Sapogenins

8.2 Methods of Analysis of Sapogenins

8.2.1 Colorimetric Methods

8.2.2 Infrared Spectrophotometry

8.2.3 Thin-Layer Chromatography

8.2.4 Column and High-Performance Column Chromatography

8.2.5 Gas Chromatography

8.2.6 Miscellaneous Methods

8.3 Problems in the Analysis of Sapogenins

8.3.1 Purity Control of Bulk Sapogenins

8.3.2 Determination of Sapogenins in Plants

References

9. Miscellaneous Steroids

9.1 Ecdysones

9.2 Solasodine and Related Alkaloids

9.3 Pancuronium Bromide and Related Quaternary Ammonium Compounds

References

Subject Index

Description

Studies in Analytical Chemistry, Volume 5: Quantitative Analysis of Steroids covers the pharmaceutical aspects of the analysis of steroid hormones. This book is divided into nine chapters that examine the biological-clinical analysis of other important groups of steroids, including sterols, vitamin D, bile acids, cardiac glycosides, and sapogenins.

The material in the book is classified according to the main groups of steroids, individual chapters being devoted to sex hormones, corticosteroids, sterols, vitamins D, bile acids, cardiac glycosides, sapogenins, and miscellaneous steroids. The structure of each chapter is the same. In section 1 the fundamentals of the chemistry of the group of steroids in question are outlined. Section 2 provides a detailed description of the use of various spectroscopic, chromatographic, protein-binding and other methods, while Section 3 deals with the main problems encountered in the analysis of the group of steroids in question and with their solution using the methods described in Section 2.

This book will prove useful to steroid and analytical chemists.


Details

Language:
English
Copyright:
© Elsevier Science 1983
Published:
Imprint:
Elsevier Science
eBook ISBN:
9780444597526

Reviews

@qu:This is a very comprehensive and well-written text. The variety of methods for the analysis and quantitation of several main groups of steroids... are covered extremely well. This book will be of great use to chemists who need to design, understand, and explore the quantitation of the families of steroids covered. @source: Analytical Chemistry


About the Authors

S. Görög Author

Affiliations and Expertise

Chemical Works of Gedeon Richter Ltd., PO Box 27, H-1475 Budapest, Hungary