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Part 1. Amino Acids
1.1. Gas Chromatography
1.1.1. Quantitation of Amino Acids as Chloroformates - A Return to Gas Chromatography (P. Hu&sbreve;ek).
1.1.2. Quantitation of Amino Acids by Gas-Liquid Chromatography (C.W. Gehrke).
1.1.3. Chiral separations of Amino Acids by Gas Chromatography (R. Pätzold, H. Brückner).
1.2. High Performance Liquid Chromatography
1.2.1. HPLC of Amino Acids without Derivatization (C. Elfakir).
1.2.2. HPLC of Amino Acids as Phenylthiocarbamoyl Derivatives (I. Molnár-Perl).
1.2.3. HPLC of Amino Acids as o-Phthalaldehyde Derivatives (I. Molnár-Perl).
1.2.4. HPLC of Amino Acids as Chloroformate Derivatives (B. Josefsson).
1.2.5. HPLC of Amino Acids as Dansyl and Dabsyl Derivatives (Toyohide Takeuchi).
1.2.6. Quantitation of Amino Acids as 6-Aminoquinolyl-N-hydroxysuccinimidyl Carbamate Derivatives (S.A. Cohen).
1.3. Capillary Electrophoresis/Capillary Electrochromatography
1.3.1. Determination of Underivatized Amino Acids by Capillary Electrophoresis and Capillary Electrochromatography (C.W. Klampfl).
1.3.2. Quantitation of Amino Acids as o-Phthalaldehyde derivatives (Shigeyuki Oguri).
1.3.3 Capillary Electrophoresis and Capillary Electrochromatography of Amino Acids as Dansyl Derivatives (Zilin Chen).
Part 2. Amines
2.1. Gas Chromatography
2.1.1. Gas Chromatographic Determination of Volatile Aliphatic and Selected Aromatic Amines, without Derivatization: Solid Phase Microextraction (J. Namiesnik, B. Zigmunt).
2.1.2. Gas Chromatography of Amines as Various Derivatives (Hiroyuki Kataoka).
2.2. High Performance Liquid Chromatography
2.2.1. HPLC of Amines as o-Phthalaldehyde Derivatives (I. Molnár-Perl).
2.2.2. Quantitation by HPLC of Amino Acids as Dansyl Derivatives (M. Silva).
2.2.3. HPLC of Amines as 9-Fluorenylmethyl Chloroformate Derivatives (Paul Chi Ho).
2.2.4. HPLC of Biogenic Amines as 6-Aminoquinolyl-N-hydroxysuccinimidyl Derivatives (T.S. Weiss).
2.3. Capillary Electrophoresis/Capillary Electrochromatography
2.3.1. Determination of Underivatized Amines by CE and CEC (C.W. Klampfl).
2.3.2. Quantitation of Amines by Oncolumn Derivatives with o-Phthalaldehyde by CEC (Shigeyuki Oguri).
3. Quantitation of Amino Acids and Amines, Simultaneously (I. Molnár-Perl).
4. Quantitation of Polyamines by Chromatography (Y. Mengerink).
Quantitation of Amino Acids and Amines by Chromatography: Methods and Protocols is intended to serve as a ready-to-use guide for the identification and quantification of amino acids and amines in various matrices, providing an overview on the theory and protocol of available methods. It presents chromatograms with exact elution programs enabling visual analysis and compares the advantages-disadvantages of various chromatographic techniques. In accordance with the chronological order of the development of chromatographic methods, different techniques are discussed: The possibilities of gas chromatography (GC), followed by those of the high performance liquid chromatography (HPLC) and the most recent techniques capillary electrophoresis (CE), capillary, electrochromatography (CEC). The characteristics of the given chromatographic procedure, relating to the topic in question, are classified according to the preliminary preparation/derivatization process(es), which means the simple methods, suitable for the analysis of the selected compound(s) in natural form, are followed by various derivatization proposals. Detailed protocols provide the reader with guidance in beginning tasks and on how to improve current methods. This book appeals to a wide audience and is recommended for those looking towards the wider reaches of identification and quantification of amino acids and amines.
- Provides a systematic, and comprehensive summary of chromatographic techniques and derivatization processes
- Compares advantages/disadvantages of various chromatographic techniques
- Readers can undertake practical tasks using detailed protocols given in the book
Students and post graduates, researchers in universities in academia and research Laboratories; Practitioners and consultants in environmental testing laboratories and the food and pharmaceutical industry
- No. of pages:
- © Elsevier Science 2005
- 27th June 2005
- Elsevier Science
- Hardcover ISBN:
- eBook ISBN:
* Provides a systematic, and comprehensive summary of chromatographic techniques * Includes a comprehensive list of chromatography of amino acids and amines * Readers can undertake practical tasks using detailed protocols given in the book
Department of Inorganic and Analytical Chemistry, L. Eötvös University, Budapest, Hungary