Qualitative Analysis of Flavor and Fragrance Volatiles by Glass Capillary Gas Chromatography

Qualitative Analysis of Flavor and Fragrance Volatiles by Glass Capillary Gas Chromatography deals with the application of glass capillary gas chromatography to qualitative analysis of flavor and fragrance volatiles. Topics range from gas chromatographic system requirements and retention indices to selective detectors, ancillary reactions, and gas chromatography-mass spectrometry. This text is comprised of six chapters; the first of which provides an overview of the use of glass capillary gas chromatography in qualitative analysis of flavor and fragrance volatiles. The discussion then turns to gas chromatographic system requirements with respect to columns, inlets, detectors, and temperature control. The reader is also introduced to the Kovats retention index system, which utilizes a logarithmic scale to describe the retention behavior of a compound relative to that of the n-paraffin hydrocarbons. The use of selective detectors, such as thermal conductivity, flame ionization, nitrogen-phosphorus, electron capture, and photoionization detectors in qualitative analysis, is also considered. The final two chapters focus on ancillary reactions in glass capillary gas chromatography and general considerations in gas chromatography-mass spectrometry, including interfacing. This book is intended primarily for researchers involved in studies of the volatile constituents of fragrances, food, and natural products.

Preface

Part A Analytical Considerations

I Introduction

II Gas Chromatographic System Requirements

A. Columns

B. Inlets

C. Detectors

D. Temperature Control

III Retention Indices

A. General Considerations

B. Determination of Holdup Time

C. Effect of Liquid Phase and Surface Pretreatment

D. Effect of Temperature

E. Isothermal Procedures

F. Programmed Temperature Procedures

IV Selective Detectors

A. General Considerations

B. Thermal-Conductivity Detector

C. Flame-Ionization Detector

D. Nitrogen-Phosphorus Detector

E. Electron-Capture Detector

F. Flame-Photometric Detector

G. Photoionization Detector

V Ancillary Reactions

A. General Considerations

B. External Reactions

C. Reaction Gas Chromatography

VI Gas Chromatography-Mass Spectrometry

A. General Considerations

B. Interfacing

Part B Appendices

Appendix I Compounds and Their Retention Indices

Appendix II Retention Indices in Increasing Order on Methyl Silicone OV101

Appendix III Retention Indices in Increasing Order on Polyethylene Glycol Carbowax 20M

Appendix IV Mass Spectra of Individual Compounds

Index