COVID-19 Update: We are currently shipping orders daily. However, due to transit disruptions in some geographies, deliveries may be delayed. To provide all customers with timely access to content, we are offering 50% off Science and Technology Print & eBook bundle options. Terms & conditions.
Protocols in Biochemistry and Clinical Biochemistry - 1st Edition - ISBN: 9780128220078

Protocols in Biochemistry and Clinical Biochemistry

1st Edition

0.0 star rating Write a review
Authors: Shweta Pandey Shyamal Goswami Buddhi Prakash Jain
Paperback ISBN: 9780128220078
Imprint: Academic Press
Published Date: 1st November 2020
Page Count: 200
Sales tax will be calculated at check-out Price includes VAT/GST

Institutional Subscription

Secure Checkout

Personal information is secured with SSL technology.

Free Shipping

Free global shipping
No minimum order.

Description

Protocols in Biochemistry and Clinical Biochemistry offers clear, applied instruction to fundamental biochemistry methods and protocols, from buffer preparation to nucleic acid purification, protein, lipid, carbohydrate, and enzyme testing, and clinical testing of vitamins, glucose and cholesterol levels, among other diagnostics. Each protocol is illustrated with step-by-step instructions, labeled diagrams, and color images, as well as a thorough overview of materials and equipment, precursor techniques, safety considerations and standards, analysis and statistics, alternative methods and troubleshooting.

Key Features

  • Includes full listings and discussion of materials and equipment, precursor techniques, safety considerations and standards, analysis and statistics, alternative methods and troubleshooting
  • Features clear, step-by-step protocols and instructions with color diagrams and images

Readership

Active researchers in biochemistry, molecular biology, and cell biology

Table of Contents

Unit I: Buffer, solution and pH
1. Preparation of normal and molar solution
2. Buffer preparation
3. Determination of pKa, pH

Unit II: Nucleic Acids
1. Isolation and Purification of DNA
2. Isolation and Purification of RNA
3. Purification of Nucleic Acid
4. Quantitative estimation of Nucleic acid
5. Agarose gel electrophoresis for separation of nucleic acid

Unit III: Carbohydrate and Lipids
1. Qualitative test for carbohydrate (Benedict, Molisch etc)
2. Qualitative test for Lipids
3. Thin liquid chromatography (TLC for separation and identification of carbohydrate and lipids

Unit III: Proteins
1. Isolation of Protein
2. Qualitative test of Protein (Biuret test, Xanthoproteic test, Ninhydrin test etc)
3. Estimation of Protein (Bradford, Lowry etc)
4. Paper Chromatography for separation and identification of amino acid
5. Ion exchange chromatography for separation of amino acid
6. Protein purification
7. SDS PAGE analysis of proteins
8. Western blotting for protein analysis
9. Isoelectric pH determination

Unit IV: Enzymes
1. Enzyme kinetics
2. Study of the activity of salivary amylase and the effect of pH, temperature, salts on its activity
3. Enzymatic activity of Trypsin and Lipase
4. Enzyme purification
5. Determination of Km, Vmax and Ki

Unit V: Clinical test
1. Acid and Alkaline phosphatase activity
2. SGPT and SGOT test
3. Estimation of Urea, Uric acid and Creatinine
4. Estimation of Glucose
5. Estimation of Cholesterol
6. Estimation of Vitamins
7. Glucose tolerance test
8. Estimation of Ca2+ and T4 in serum
9. Pregnancy test by HCG hormone
10. Estimation of Glycosylated haemoglobin

Details

No. of pages:
200
Language:
English
Copyright:
© Academic Press 2021
Published:
1st November 2020
Imprint:
Academic Press
Paperback ISBN:
9780128220078

About the Authors

Shweta Pandey

Dr. Shweta Pandey is an Assistant Professor at the Government P G College, Chhatishgarh, India.

Affiliations and Expertise

Assistant Professor, Government P G College, Chhatishgarh, India

Shyamal Goswami

Dr. Shyamal K. Goswami is a Professor at the School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.

Affiliations and Expertise

Professor, School of Life Sciences, Jawaharlal Nehru University, New Delhi, India

Buddhi Prakash Jain

Dr Buddhi Prakash Jain is an Assistant Professor at the Department of Zoology, Mahatma Gandhi Central University Motihari Bihar India. After completing Doctoral Studies from the School of Life Sciences, Jawaharlal Nehru University (JNU), New Delhi, he continued as an academic and researcher in the area of gene expression regulation and endoplasmic reticulum stress. He was awarded with a gold medal in his master programme (M.Sc.). He is also working on various biochemical assays under heavy metal stress. He has published several papers in reputed international journals and many book chapters in edited books.

Affiliations and Expertise

Assistant Professor, Department of Zoology, School of Life Sciences, Mahatma Gandhi Central University Bihar, Motihari, India

Ratings and Reviews