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Protocol Handbook for Cancer Biology - 1st Edition - ISBN: 9780323900065, 9780323898171

Protocol Handbook for Cancer Biology

1st Edition

Editors: Gauri Misra Jyotika Rajawat
Paperback ISBN: 9780323900065
eBook ISBN: 9780323898171
Imprint: Academic Press
Published Date: 13th February 2021
Page Count: 216
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Protocol Handbook for Cancer Biology brings together a comprehensive collection of the methods used for cancer assessment, diagnostics, and therapeutics. Various protocols are discussed along with alternative strategies, including the advantages and limitations of techniques that have been used in labs globally. These protocols are presented by cancer biology experts based on their real-world experience.

The book discusses a multitude of practical topics such as:

– Cell viability, proliferation and apoptotic assays for cancer detection

– Assays and protocols to evaluate tumor angiogenesis and further detect cancer metastasis

– Isolation and characterization of cancer stem cells

– High-throughput cell-based screening methods for cancer drug

– The role of nanotechnology in cancer therapy

– Histopathological and tumor imaging protocols for cancer diagnosis and treatment.

The protocols in this book will be a valuable resource for cancer researchers and graduate students, who can utilize the techniques described to conduct research more efficiently and successfully.

Key Features

  • Presents comprehensive protocols used for cancer assessment, diagnostics, and therapeutics all in one place.

  • Encompasses alternative strategies considering the requirements of the end user and taking into consideration diverse research settings.

  • Discusses limitations and advantages of each method in experimental design and execution, thus saving time during the research process.


Cancer researchers; graduate students

Table of Contents

1. Overview of cancer assessment, diagnostics and therapeutics
2. Cell viability and apoptic assays to assess drug efficacy
3. Assays to assess the proliferative behavior of cancer cells
4. Assays to evaluate tumor angiogenesis
5. Assays and protocols for the detection of cancer metastasis
6. Isolation and characterization of cancer stem cells
7. Histopathology-based cancer detection methods
8. Molecular imaging in tumor diagnosis and treatment
9. High throughput cell-based screening methods for cancer drug discovery
10. Nanotechnology in cancer therapeutics: cerium oxide nanomaterials as cancer therapeutic agents



No. of pages:
© Academic Press 2021
13th February 2021
Academic Press
Paperback ISBN:
eBook ISBN:

About the Editors

Gauri Misra

Gauri Misra, PhD, has a research focus in the field of cancer biology and Malaria directed toward drug discovery, armored with an experience in Structural Biology, Biophysics and Bioinformatics. She has an outstanding academic background and experience of more than 10 years in the field of teaching and research, adorned by various prestigious awards and honors at different stages of her career with a strong desire of transferring her repertoire of knowledge to the upcoming generations. Her PhD thesis was awarded with Eli Lilly Asia Outstanding Thesis Award (first prize) in 2009. She has received “state of the art” International exposure while working as a postdoctoral researcher at CHUL Research Centre, Canada and visiting scientist at ISPC, Weizmann Institute of Science, Rehovot, Israel in 2014 along with few other countries. She serves as a reviewer for various renowned international journals and is often invited for speaking engagements about her research. To date, she has authored 17 research articles, 4 international books, and 6 book chapters. The ongoing research project in her laboratory focuses on evaluating the potential of new lead compounds against breast cancer.

Affiliations and Expertise

Scientist Grade-II, National Institute of Biologicals, (Autonomous Institute under Ministry of Health and Family Welfare), Government of India, Noida, Uttar Pradesh, India

Jyotika Rajawat

Jyotika Rajawat, PhD, researches in the field of cancer and drug discovery. She has a rich experience of 8 years in cancer research and teaching. Her postdoctoral research at CHUQ Research Centre, Canada, followed by research projects at National Centre for Cell Science (Pune), CSIR- Central Drug Research Institute (Lucknow), India has established a novel role of PARP1 protein in cancer progression. She has written several research articles, three book chapters, and newspaper articles. She is a member of the Editorial Board, IJBST journal and is a reviewer for various international publishers. She has been the recipient of several awards including DBT Bio-CARe and DST Women Scientist Award (awarded by the Govt. of India). She is also passionate about conducting cancer awareness workshops and literacy camps for the underprivileged especially in the rural areas. Dr. Rajawat being an expert in cancer biology understands the needs of readers and writes with those needs in mind.

Affiliations and Expertise

Research Scientist, DST WOS-A project, Lucknow, Uttar Pradesh, India

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