Protides of the Biological Fluids - 1st Edition - ISBN: 9780080355887, 9781483148380

Protides of the Biological Fluids

1st Edition

Proceedings of the Thirty-Fifth Colloquium, 1987

Editors: H. Peeters
eBook ISBN: 9781483148380
Imprint: Pergamon
Published Date: 1st January 1987
Page Count: 556
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Protides of the Biological Fluids examines protides of the biological fluids and covers topics ranging from the use of DNA probes to diagnose inherited diseases and receptors to the conformation and function of biologically active peptides. This text has 115 chapters and begins by demonstrating the existence of gene families common to several vertebrates and which evolved by intragenic duplication. The chapters that follow focus on the use of DNA probes in the analysis of inherited disorders such as thalassemia and hemophilia. The reader is then introduced to receptors, especially for peptides. Receptors on circulating cells, hormone receptors, receptors involved in cancer, and immunoglobulin receptors are explored. The section on the conformation and function of biologically active peptides considers the methods including spectroscopic methods, crystallography, and theoretical conformational analysis. In particular, the use of synchrotron X-radiation in biological crystallography and of 2D NMR spectroscopy in the identification of folded structures in immunogenic peptides is highlighted. This book will be of value to biologists and biochemists.

Table of Contents



The Sixteenth Arne Tiselius Memorial Lecture

Expression of Genes Encoding the Vitamin D Binding Protein and Transferrin

Section A. DNA Probes For Inherited Diseases

A.1. Thalassemia

Diagnosis of Thalassemia by DNA Analysis

A.2. Haemophilia and X-Linked Diseases

First Trimester Prenatal Diagnosis of Hemophilia A by Combined Use of DNA Probes

Carrier Detection and Genetic Counselling in Hemophilia Using DNA Probes

The Identification of Haemophilia B Mutations

Gene Defects in Haemophilia A and B

Carrier Detection Using DNA Analysis in French Families of Haemophilia A and B

Studies on the Molecular Basis of Steroid Sulphatase Deficiency in Man

Mitotic-Origin of a Duchenne Muscular Dystrophy Mutation

A.3. Unknown Genes

Progress Towards Cloning of the Cystic Fibrosis Gene—Identification of New DNA Markers in the 7Q31 Region

DNA Marker Analysis of Danish Polycystic Kidney Disease Families

Information Given by the 3' HVR α-Globin Marker in Ten French Families with Adult Polycystic Kidney Disease

Use of a Genetic Marker for the Diagnosis of Adult Polycystic Kidney Disease in Northern Italy

Construction of Lambda gt 11 cDNA Libraries from Post-Mortem Human Brains

Genetic Analysis of Human T-Cell Antigens. I. Cytogenetic and Phenotypic Characterization of Interspecies T-Cell Lines

Genetic Analysis of Human T-Cell Antigens. II. Mapping of the Human Chromosomes Involved in the Expression of T10 T-Cell Antigen

A.4. Multifactorial Diseases

Genetic Approach to the Study of Multifactorial Diseases

Restriction Fragment Length Polymorphisms in the Apolipoprotein E-C1-C2 Gene Cluster: Association with Familial Dysbetalipoproteinemia

The Involvement of Apolipoprotein B Gene Variants in the Determination of Serum Cholesterol Levels

The Relevance of the Use of Synthetic Oligonucleotides for Apolipoprotein E (apoE) Mutants for Clinical Diagnosis of Hyperlipoproteinemia Type III

DNA Markers Linked to Diabetes

HLA Class II Region α Chain Polymorphism and the Genetic Susceptibility to Insulin Dependent (Type 1) Diabetes Mellitus and Coeliac Disease

A.5. Other Proteins and Amino-Acids

Correlation Between Polymorphic DNA Haplotypes at the Phenylalanine Hydroxylase Locus and Clinical Phenotypes of Phenylketonuria

Genetic Variants of Serum Albumin and Polymorphism of the Albumin Gene

Identification of Carriers of Mutant Prealbumin Gene Associated with Familial Amyloidotic Polyneuropathy Type I: Study of One Portuguese Pedigree

Synthesis of Serum Amyloid A by Murine Hepatocytes and of Amyloid-Related Protein(s) by Murine Peritoneal Macrophages

Unique DNA Polymorphisms Associated with the Alpha1-Antitrypsin (PI) Z Deficiency Allele: Application to Prenatal Diagnosis

High Frequency of Arginine 47 Mutation in Antithrombin III Hereditary Deficiencies Affecting the Heparin Binding Site

Studies of DNA Polymorphisms in Collagen Genes in Health and Disease

Isolation and Characterization of a cDNA Clone for Human Alpha2-HS Glycoprotein

Section B. Receptors

B.1. Neuropeptide Receptors

Further Characterization of Two 3H-Neurotensin Binding Sites in Rat Brain

Biochemical and Cellular Characterisation of Multiple Tachykinin Receptors

Regulation of μ-Opiate Receptors in Cultured Neurons

3H-Lofentanil Binding to Opiate Receptors in Human Cerebellum

Distribution in the Brain and Spinal Cord (Rat and Human) of Enkephalin Targets (μ, δ Opioid Receptors and Enkephalinase) in Various Physiopathological Conditions

Cholecystokinin (CCK) Receptors and Immunoreactivity in the Rat Brain: Functional Significance of Subregional Distributions

Modulation of Intracranial Self Stimulation by Cholecystokinin Depends on the Site of Administration

Modulation of Natural Killer (NK) Cell Activity by Met-Enkephalin in Culture

Autoradiographic Localization of CCK Binding Sites in the Human Brain

Characterization of Substance P (1-7) Generating Enzyme in Human Choroid Plexus

Solubilisation of the Muscarinic Receptor in CHAPSO: A Comparison with the Receptor in Membranes and in Digitonin Solution

Anti-Idiotypic Antibodies that Bind Cholinergic Ligands Isolated from a Myasthenia Gravis Patient

Cellular Response to Acetylcholine Receptor in Myasthenia Gravis

Degradation of Leu- and Met-Enkephalin and their C-Terminal Extensions by Tripeptidyl Peptidase II

B.2. Receptors on Circulating Cells

Structure and Function of GPIb, The Principal Platelet Von Willebrand Receptor

Secretion of ir-ACTH by Cells of Lymphoid Origin: Detection at the Single Cell Level

Cytotoxic Activity of Lymphocyte Clones from Aged People

Proliferative Capacity of Lymphocyte Clones from Old Subjects

Modulation of Target Molecules by γ-Interferon: Susceptibility of Lysis by NK Cells

Laminin Production by Natural Killer Cells

Agonist Function of Anti-CD2 and Anti-CD16 Monoclonal Antibodies on Human NK Killing

Flow Cytometric Evaluation of Natural Killer Cytotoxicity

Freeze-Fracture Ultrastructure of the Binding Between NK Lymphocyte and K562 Target Cells

The Low-Affinity Fc Receptor for IgG Recognized by MOAB AB8.28 is Capable of Transducing Activation and Degranulatory Signals in Large Granular Lymphocytes

Binding of C-Reactive Protein and Related Peptides to Neutrophils

The Phosphatidylinositol-Specific Phospholiphase C (PLC) in Rat Neutrophils (PMN)

Subcellular Localization of Mac-1 (CR3) in the Human Neutrophil

Polymorphonuclear Cell Fc-Gamma Receptors in Rheumatoid Arthritis

Formylpeptide Induced Neutrophil Chemotaxis Inhibition by Atropine and Phentolamine

Receptors for Tuftsin on Plasma Membranes of Macrophages and Macrophage Hybridoma Cell Lines

Analysis of Cell Mediated Immune Response by Using Interspecies Hybrid Lines as Stimulator

The Purification of Human α1-T Glycoprotein from Serum and its Clinical Application

Role of Fibronectin in the Function of Neutrophils

B.3. Hormone Receptors

Studies on Growth Hormone Receptors

Characterization of the Somatogenic Receptor in Rat Liver

Characterization of Rat Liver Prolactin Receptor

Characterization of Angiotensin Receptors from Human Kidney

Insulin Binding on Erythrocytes is Increased by Albumin-Associated Plasma Modulators

Insulin Like Growth Factor 1 Receptor on Isolated Lymphocytes as a Basis for Studies of IGF-1 Receptors on Mitogen-Stimulated-Lymphocytes and Lymphatic Cells

Which Method for "Microalbuminuria" In Diabetes Mellitus?

B.4. Receptors in Cancer

Heterogeneity of Oestrogen Receptors in Human Breast Tumors

Clinical Significance of Abnormal Oestrogen Receptors in Breast and Gynaecological Cancers

Are Epidermal Growth Factor Receptors Present in the Human Prostate

Antiproteasic Peptides in Human Cancer: A Host Reaction or an Autonomic Secretion

Biological Expression of Proteinase Inhibitors Pattern in Human Cancer. The Clinical Potential Utility

Secretion of Two Different Types of Growth Factors by the HT-29 Human Colonic Adenocarcinoma Cell Line

Fibroblast Aromatase: A New System for Investigating the Mechanisms of Action of Vitamin D Metabolites on Cellular Differentiation

Comparison of Prostate Specific Antigen and Prostatic Acid Phosphatase in the Detection of Prostatic Cancer

Behavior of Serum Antiproteasic Enzymes in Relation with Chemotherapy Regimens

Effect of Tumor's Metastatic Potential and Growth Rate on Tumor/Host Relationship

The Utilization of Monoclonal Antibodies to Detect Alterations in Leukocytic Subset Ratios Following Chemotherapy

Effect of Tumor Necrosis Factor on a Prostate Tumor Model

Evaluation of Adriamycin Chemotherapy and Polyamine Synthesis Inhibitor Alpha-difluoromethylornithine (DFMO) in the Treatment of a Transplantable Prostate Carcinoma of the Rat

B.5. Immunoglobulin Receptors

Serum Levels of Free and Ligand-Bound Receptor for Polymeric Immunoglobulins in Bile Duct-Ligated Rats

Immunoglobulin (IG) Receptors of Renal Tubule: Subcellular Localization and Characterization

A Monoclonal Antibody Interacting with Human Fcγ Receptor

Human Fcγ Epitopes and Interaction Sites for Effector Molecules

Occurrence of Damaged Heavy Chains During Purification of Murine IgE Antibodies by Fast Protein Liquid Chromatography (FPLC) and Their Effect on the Determination of Concentration and Affinity in ELISA

B.6. Biomatrix

Cooperation of Kupffer Cells and Perisinusoidal Lipocytes in the Production of Liver Biomatrix Components: Mediators of Kupffer Cells Stimulate Proliferation and Proteoglycan Synthesis of Lipocytes

Interactions of Free Glycosaminoglycan Chains and of Native Proteoglycans Secreted by Rat Liver Fat Storing Cells with Hepatocytic Surfaces

Epithelial Cell Surface Proteoglycan: A Matrix Receptor for Interstitial Collagens and Fibronectin

Properties of High and Low Molecular Weight Phosphomannose Receptors from Bovine Liver and Testis

Fibrinogen Binding on Candida albicans Germ Tubes and Mycelium

Receptor-Mediated Uptake of B-Glucuronidase into Glial Cells from Rat Brain

Cell Fusion in Electric Field in the Production of Monoclonal Antibodies Against Soluble and Cellular Polypeptide Antigens

Type IX and Type XII Collagens: A New Class of Extracellular Matrix Components

B.7. Other Receptors

The Use of Specific Antisera to Study the Developmental Regulation of Guanine Nucleotide Binding Proteins

Identification of Bradykinin Receptors on NG108-15 Hybrid Cells

Section C. Conformation And Function Of Biologically Active Peptides

C.1. Molecular Graphics

The Use of Molecular Graphics in the Design of Inhibitors of Zinc Proteases

New Techniques for the Exploration of Molecular Energy Surfaces and their Application to Dermorphin

C.2. NMR Studies

Two-Dimensional Exchange Experiments in NMR Studies of Protein Dynamics and Folding

Properties of the Protein Solvent Interface, Studied by Deuterium Exchange in Basic Pancreatic Trypsin Inhibitor

A Comparative Molecular Structural Study of the Third Component of Complement and Alpha-2-Macroglobulin: Structures of the Peptide Segments that are Susceptible to the Attack by Endoproteinases

Dynamics of Elastase Peptide Complexes. A 13C NMR Study

The Determination of the Three Dimensional Structure of Human Epidermal Growth Factor from 1H-NMR Data

Conformational Comparison of Four Constrained Stereoisomeric Cyclic Enkephalin Analogs by 1H NMR and Computer Simulations

Opioid Peptides in Micellar Systems: Conformational Analysis by 1D and 2D 1H-NMR and Circular Dichroism

Long, Chiral Polypeptide 310-Helices

C.3. X-Ray Studies

Uses of Synchrotron X-Radiation in Biological Crystallography

X-Ray Crystallography of Linear Peptides Through the Renin-Angiotensin System

C.4. Conformation in Solution

Identification of Folded Structures in Immunogenic Peptides by 2D NMR Spectroscopy

Conformational Properties of Calcitonin and their Relationship to Biological Activity

On the Difference Between Molecular Conformation and Hydrogen Bonding in Solution and in Crystalline State

Antiprotease Engineering: New Protease Inhibitors for Therapeutic Use

Enkephalinase, A Metalloendopeptidase Involved in the Inactivation of Biologically Active Peptides

Neutron Scattering Analysis of Angiotensin-Converting Enzyme (ACE)

C.5. Antigen-Antibody Binding

Immunochemical Study of Cyclosporine Conformation in Aqueous Medium

Localization of Two Epitopes with Monoclonal Antibodies in the Cell Attachment Domain of Fibronectin by Synthetic Peptides

Immunoreactivity and Biological Activity of Thymulin Analogs Studied by EIA and Rosette Assay

Affinity Constant and "In Vitro" Biological Activity of Human Monoclonal Antibodies Against Rh (D) Antigen

Contiguous and Discontiguous Antigenic Determinants on Proteins

Author Index

Subject Index


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About the Editor

H. Peeters

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