Protides of the Biological Fluids - 1st Edition - ISBN: 9780080332154, 9781483279763

Protides of the Biological Fluids, Volume 33

1st Edition

Proceedings of the Thirty-Third Colloquium, 1985

Editors: H. Peeters
eBook ISBN: 9781483279763
Imprint: Pergamon
Published Date: 1st January 1985
Page Count: 722
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Table of Contents



The Fourteenth Arne Tiselius Memorial Lecture

Progress in Plasma Proteins: Structure, Function, and Genes

Section A. Plasma Protein Genes

A.1. Gene Structure

Organization and Chromosomal Locations of Genes Encoding Human Plasma Proteins

Sequence Analysis of Human Immunoglobulin Heavy-Chain Genes

Structure and Evolution of the Serum Albumin Gene Family

Molecular Genetics of Human Transferrin

Apolipoprotein A-II Gene Structure: Evolutionary Implications

Molecular Genetics of Haptoglobin

Human Complement Genes of the Major Histocompatibility Complex

Coagulation and Fibrinolysis: Characterization of the Human Prothrombin and Human Tissue-Plasminogen Activator Genes

The Presence of Multiple Polyadenylation Sites in the Gene for the β and γ Chains of Human Fibrinogen

The Human Factor VIII Protein

Structure of the Gene for Human Factor IX

Structure and Evolution of the Human Antithrombin III Gene

The Structure of the Human Plasminogen Activator (uPA) Gene

A.2. Chromosomal Location

Chromosome Mapping of Serum Protein Genes

Cloning and Expression in Micro-Organisms of the Genetic Information Coding for Human Plasma Proteins

In Situ Hybridization for Chromosomal Mapping of Plasma Proteins

In Situ Hybridization: Location of Plasma Protein Transcription

Structure and Liver Specific Expression of the Human Retinol Binding Protein Gene

Organization of the Genes Coding for Prothrombin and Factor X

Mapping Tissue Plasminogen Activator and Urokinase Genes to Human Chromosomes

Molecular Genetics of Human Antithrombin III Deficiencies

Genetically Abnormal Human Fibrinogens — A Summary of 32 Structurally Elucidated Variants

Linkage of Human Apolipoprotein AI, CIII and AIV Genes

Genetic Polymorphisms and Linkage Relations of Complement Factor C8

Close Genetic Linkage Between Four Plasma α-Protease Inhibitor Loci (Pi1, Po1A, Po1B, Pi2) in Pigs

Genomic Evidence for the Truly Allelic Nature of the B-Locus Polymorphism of the Rabbit Immunoglobulin Light Chain

A.3. Cloning and Expression

Genetic Studies on a Human Plasma Transthyretin (Prealbumin) Variant Associated with Familial Amyloidotic Polyneuropathy

Gene Organization and DNA Polymorphism of the Human Transferrin Gene

Expression in E. coli of Human Preprourokinase cDNA

Cell Cycle Dependent Variations in the Distribution of the Nuclear Protein Cyclin (PCNA) in Cultured Cells

Organization and Evolution of the Mouse Complement C4 Isotypes

Altered Inhibition Properties of α1-Antitrypsin Variants Constructed by Site-Directed Mutagenesis

Structure of the Human Haptoglobin Pseudogene

Molecular Cloning, Sequencing and Expression of a cDNA Coding for Human Antithrombin III

Purification of Active Human α1-Antitrypsin From Genetically Engineered Micro-Organisms

Regulation of Rat α2-Macroglobulin Gene Activity

Influence of Neonatal Androgenization on the Expression of α2u-Globulin Genes

Preferential Segregation of Murine Chromosome 11 in TCGF-Independent Rat x Mouse T-Cell Hybrids

Individual Polymorphism of Albumin Gene Studied by Sequencing DNA

Section B. Antinuclear Antibodies (ANA)

B.1. Chemistry of Nuclear Antigens

Fine Structure of snRNPs

Anti-Sm and Anti-La Murine Monoclonal Antibodies: Characterisation of the Antigens

Antihistone Antibodies Bind to Restricted Epitopes

IP-Heterogeneity of Nuclear Antigens Detected in an Isoelectric Focusing-Immunoblot System

Characterization of Nuclear and Cytoplasmic Autoimmune Antigens

Analysis of Nuclear and Cytoplasmic Protein Antigens in Systemic Rheumatic Disease

Interaction of VA RNA with the LA Antigen of HeLa Cells and Frog Oocytes

Characterization of an Ubiquitous S-Phase Related and Chromatin Associated Antigen with Human Autoantibodies

A 80 KD Protein is Associated with the Nucleolus Organizer (NOR) of Human Cell Lines

Polymorphism of Epstein-Barr Virus Nuclear Antigen (EBNA)

Immunochemical and Biological Properties of Anti-Nucleic Acid Antibodies in SLE

Recognition of dsDNA by Anti-DNA Antibodies. Immune Complex Formation and Further Potential Biological Consequences

Crossreactivity of Monoclonal Antibodies to DNA: Related to Avidity?

Diversity and Multispecificity of Autoantibodies Reactive with DNA: Some Evolutionary Implications

Isolation and Characterization of HP10: A Specific Human Hepatic Protein cDNA

ANA With Dual Specificity for the Histone Octamer and a Plasma Membrane Antigen React with H2B in Immunoblot but not in Immunosedimentation

Biochemical Mechanism of Chromosome Banding Analysed by Monoclonal Anti-DNA Antibody

Immune Response of Genetically Normal Mice Towards Isolated Native U1 Small Nuclear Ribonucleoproteins: Evidence that U snRNPs may Function as an Immunogen During the Anti-RNP/Sm Autoantibody Response

Auto-Antigens of Nuclear Ribonucleoprotein Particles

The Antigenic Structure of Histones

Immunochemical Study of Post Synthetic Histone Modifications on Chromatin Conformation

Immunohistochemical Study During Phylogenesis of a Nervous Tissue Subnuclear Protein Fraction

Monoclonial Antibodies and Human Autoantisera Used for the Detection and Identification of Nuclear Matrix Compounds

Extracellular Release of Nuclear SSB-Antigen by Adenovirus Infection and Interaction with Anti-SSB Antibody

Autoantibodies to Highly Purified Nuclear Proteins in Aging

B.2. Autoantibodies in Disease

B.2.1. SLE

Autoantibody-Profiles in Patients with SLE, Scleroderma and Overlap-Syndromes

Anti-ENA Antibodies in Systemic Lupus Erythematosus: Clinical-Serological Correlations

The Qualitative and Quantitative Aspects of Anti-dsDNA Antibodies in Systemic Lupus Erythematosus

Role of Sex Steroids in Systemic Lupus Erythematosus (SLE)

Cross-Reactivity of Anti-DNA Antibodies: A New Clue for the Pathogenesis of SLE?

Anti-ENA Antibodies in Systemic Lupus Erythematosus: A Follow-Up Study

Complement, IgA, CNS and Mortality in SLE

Clinical Differences Between S.L.E. Patients in Relation to the Avidity of their Anti-dsDNA

Antibodies to the Histones: Different Patterns of Response in Idiopathic SLE and Drug-Induced Lupus

Antibodies Against Phospholipids (Cardiolipin) in SLE

Clonal Analysis of Autoantibodies in Autoimmune Thyroiditis and SLE

Multiple Ligand Binding by Sera From Systemic Lupus Erythematosus Patients

B.2.2. Other Diseases

The Immunopathogenesis of Primary Sjögren's Syndrome

Autoantibodies in Sjögren's Syndrome: Comparison of Autoantibody Determination Methods Show that Antinuclear Antibody and Rheumatoid Factor are Associated with Ro/SSA Precipitin Formation

Autoantibodies to Transfer RNA-Related Antigens in Myositis

Diversity of Antinuclear Antibodies in 42 Cases in Polydermatomyositis (PM/DM)

Are Anti-nRNP Levels Specific Guides for Assessing Disease Activity in Patients with (Mixed) Connective Tissue Disease?

Diagnostic and Prognostic Significance of Anti-Centromere Antibodies (ACA)

Nuclear Antibodies in Autoimmune Liver Diseases Defined by Immunoprecipitation and Cell Culture Substrates

Antiphospholipid Antibodies in Chronic Active Hepatitis: Lack of Correlation with Anti-DNA Antibodies

Soluble Immune Complexes (SIC), Rheumatoid Factor (RF) and Auto-Antibodies in Leprosy Patients' Sera

Antigenic Heterogeneity as Reflected by Interaction with Antibody (Ab) and Circulating Immune Complexes (CIC) in Sarcoma Patients

B.3. ANA Determination

B.3.1. Detection of Autoantibodies

The Detection of Autoantibodies: An Overview

Ultrastructure of HEp-2 Cells

Use of HEp-2 Cells in the IF Test for Detection of Antinuclear Antibodies

Correlation of Albumin mRNA Levels with Secretion Rates in Fetal Rat Hepatocyte Cultures Exposed to Dexamethasone

What is the Primary Target of Anti-DNA Auto-Antibodies?

B.3.2. Assays

Quantitation of Antibodies to Nucleoribonucleoprotein (nRNP) by ELISA: Relation Between Antibody Levels and Disease Activity in Patients with Connective Tissue Disease

Immunoblotting as a Diagnostic Tool in Connective Tissue Diseases

Immunoblotting with Nuclear Antigens: A Study of the Diagnostic Value

ELISA for Antibodies to Ro-Antigen. Diagnostic Value, Compared to Antibodies to dsDNA, Histones, RNP, Sm- and SSB-Antigen

Affinity Purification and Immunoassay of Anti-Ro/SSA

A Practical Quantitative Method for Specific Antinuclear Antibodies

Application of the Dot Immuno-Binding Assay to Connective Tissue Disease

A Comparison of Three Methods for the Assay of Anti-DNA Antibodies

Enzyme Immunoassay for the Detection of Autoantibodies Against the Nuclear Protein Scl-70

Single Radial Immune Haemolysis (SRIH) Assay for the Measurement of Complement Fixing Antibodies

Section C. Methodologies

C.1. Isoelectric Focusing (IEF)

C.1.1. Unidimensional (1D)

Electrofocusing in Wide pH Range Immobiline Gels Containing Carrier Ampholytes

Patient-Specific Banding Pattern in Isoelectric Focusing-Immunoblot with Nuclear Antigens

Evaluation of Ready-Made IEF Gels for the Demonstration of IgG Oligoclonal Bands in Cerebrospinal Fluid

IEF of Albumin in Low Denaturing Media. I: Analytical Aspects Related to Uremia

IEF of Albumin in Low Denaturing Media. II: Preparative Aspects

pH Gradient Manipulation within the pH 4-6 Range Using Separator Isoelectric Focusing

C.1.2. Two Dimensional (2D)

Immobilized pH Gradients as First Dimension for 2D Separations

IEF and 2D-Electrophoresis with Narrow and Ultranarrow Immobilized pH Gradients for the Analysis of Genetic Variants

Genetic Studies of Apolipoprotein A-IV by Two-Dimensional Electrophoresis

Two-Dimensional Polyacrylamide Gel Electrophoresis of Escherichia coli Proteins with Pharmalytes

Two-Dimensional Electrophoretic Analysis of Genetic Muscle Disorders

Quantitative Immunoelectrophoretical Determination of Some "Intrathecal Synthesized" Proteins

Examinations of Monoclonal IgG with Preparative Chromatofocusing Followed by 2-Dimensional Gel Electrophoresis

Two Dimensional Immunoblot Electrophoresis for Characterization of Pancreas Specific Oncofoetal Antigens

Applications of Small-Column Chromatofocusing and 2-Dimensional Gel Electrophoresis in CSF and Serum Protein Examinations

C.2. Isotachophoresis

Isotachophoretic Analyses of Compounds in Complex Matrices: Allergenic Extracts and Aluminium in Biological Fluids and Bone

C.3. Gel Electrophoresis

Micro Gel Electrophoresis

Limits and Feasibilities of Gel Densitometry

Sieving of Rod-Shaped Viruses During Electrophoresis in Agarose Gels

Electrophoretical Separation of Urinary Alanine Aminopeptidase (=AAP) Isoenzymes in Ultrathin Agarose Gels

Horizontal Ultrathinlayer SDS Poregradientgel Electrophoresis for the Differentiation of Proteinurias

Apparatus for Fast Electroelution of Microgram Quantities of Proteins from Polyacrylamide Gels

Non-Invasive Diagnosis of Kidney Diseases Applying Sensitive Thin-Layer SDS-PAA Gradient Gel-Electrophoresis

C.4. High Performance Electrophoresis (HPE)

Analytical and Microprepartive High-Performance Electrophoresis

C.5. High Performance Liquid Chromatography (HPLC)

An Automated Tandem HPLC System Applied to Peptide Mapping of Human Plasma Proteins

Isolation and Characterisation of Fish Protamines by Reversed-Phase High-Performance Liquid Chromatography

Usefulness of a Multicolum FPLC-System for Fractionation and Monitoring of Proteins and Peptides in Uremie Serum, Hemofiltrates an Dialysates

Broad pH-Gradient Chromatofocusing

Studies on the Analysis of Brain Structural Proteins by Chromatofocusing

Size Exclusion Chromatography of Proteins on Improved Bonded Silica Columns

An Optimized Method to Measure Glycosylproteins: HPLC Assay of 5-Hydroxymethyl-2-Furfuraldehyde (5-HMF)

Reversed Phase High Performance Liquid Chromatography in Studies of the Substance P Turnover

C.6. Immunoblotting

Screening for Monoclonal Antibodies Against a Mycoplasmal Membrane Protein by Protein Electroblotting

An Economical Design of Immunoblotting Tank

Immunoblotting of Human Pepsinogens

C.7. Staining Methods

Quantitative Aspects of Protein Staining by Coomassie Blue Dyes and Silver Staining

Sensitive Protein Stain on Nitrocellulose Blots

Studies of Antithrombin III Charge Heterogeneity

Silver Staining and SDS Polyaerylamide Gel Electrophoresis of Antibacterial Proteins in Bovine and Human Milk

Immunogold Staining of Blotted Proteins — Demonstration that Fibronectin Initiates the Immunoglobulin Cryoprecipitation

Statistical Analysis of Interference Between α, β, γ Globulines and Serum Albumin Determination with Dye Binding by Bromcresol Green and Bromcresol Purple

C.8. Antibodies and Monoclonals

A Practical Method for Light Chain Quantification for Each Immunoglobulin Class

Isolation, Characterization and Quantitation of Murine IgE

Production of Monoclonal Antibodies to Dog Post-Gamma Globulin

Interaction of Human Chorionic Somatomammotropin Monoclonal Antibodies with Fragments Derived from the Nicked Form of the Hormone

Purification of a Single-Chain Urokinase Precursor from A413 Human Epidermoid Carcinoma Cells by Monoclonal Antibody Immunoaffinity Chromatography

A Simple Method for Isolating Pig Zona Pellucida and the Detection of Anti-Zona Antibody in Relation to Infertility by Immunofluorescence and Immunoenzymatic Methods

Anti-Thymocyte Antibodies in Myasthenia Gravis: Their Relation to Anti-AchR Antibodies

Analysis by Monoclonal Antibodies of Cell Surface Structures Marking Discrete Steps of Cell Differentiation

A Triple Immunoenzymatic Method for Histo-Pathological Studies: Detection of IgG, IgM and Ig A Positive Cells Simultaneously in Single Tissue Section

C.9. Other Methodological Papers

Bioreactors with Laminar and Defined Gradient Hydrodynamics: Hydraulic Culture of Cells for Assessment of Effectors Controlling Regenerative Tissue Morphogenesis in Wound Healing

Separation of Nonenzymatically Glycosylated Proteins by Phenylboronate Affinity Chromatography

Glycosylated LDL Analyzed by Electrophoretic Mobility (E.M.)

Comparison of an ELISA and a Radial Immuno Diffusion Method for the Determination of Factor VIIIR : Ag

Cellular Adhesion to Low Molecular Weight Connective Tissue Matrix Proteins

Tissue Plasminogen Activator in the Cytosol of Breast Tumors: Technical Aspects and Correlations with Commonly used Prognostic Factors

Use of Immunoprecipitates to Produce Anti-BCG Cell Wall Antibody of Restricted Specificity from Mycobacterium BCG

An Updated Method for the Purification of Mycobacterium leprae and Other Mycobacteria from Infected Tissues

Lack of Receptors for Fibronectin on Leukemic Granulocytes

Detection and Immunohistochemical Localisation of hPLAP and CA 125 in Sera and Tissues of Patients with Ovarian, Nonovarian Tumors, and Liver Disorders

Author Index

Subject Index


An International Review Series Devoted to Proteins and Related Studies, Volume 33: Protides of the Biological Fluids documents the proceedings of the 33rd Colloquium held in 1985.

This book is divided into three sections. Section A focuses on the plasma protein genes that includes the location of genes on the chromosome, pathology and variance of several genes, and gene expression. The proteins of the nucleus and detection of antibodies, such as antinuclear antibodies (ANA), to nuclear constituents are deliberated in Section B. Section C is devoted to the technical advances in the field of protein chemistry.

This book is a good reference for students, researchers, and medical practitioners conducting work on the protides of biological fluids.


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About the Editors

H. Peeters Editor