Protides of the Biological Fluids - 1st Edition - ISBN: 9780080168760, 9781483146348

Protides of the Biological Fluids

1st Edition

Proceedings of the Nineteenth Colloquium, Bruges, 1971

Editors: H. Peeters
eBook ISBN: 9781483146348
Imprint: Pergamon
Published Date: 1st January 1972
Page Count: 606
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Protides of the Biological Fluids is a compendium of papers presented at the XIX Colloquium held at Bruges in 1971. It focuses on three main topics: lipoproteins, proteins, and protein catabolism.
The main section of this book contains 60 papers discussing the composition, structure, synthesis, genetics, and function of lipoproteins. Another section is devoted to dielectric relaxation, fluorescence depolarization and determination of protein structure. The final section discusses protein catabolism and the applications of immunoelectrophoresis to protein quantitation. Students and scientists looking for an extensive reference on protein chemistry will find this book invaluable.

Table of Contents



Academic Lecture

Immunochemical Studies on Human Gastro-Intestinal Tumors

Section A: Lipoproteins


Conceptual Development of the Classification Systems of Plasma Lipoproteins

A1. Composition and Structure

Isolation of Lipoproteins from Human Serum by Precipitation with Polyanions and Divalent Cations

A Comparison between HDL Lipoproteins and Apoprotein-Phosphatidylcholine Cenapses

Comparative Studies on Different Lipid-Staining Procedures, Especially for Cellulose Acetate Electrophoresis

Characteristics of Six Fractions Obtained from Human High Density Lipoprotein

Electrofocusing of Normal Apolipoproteins and the Amino Acids of Their Subfractions

Studies on the "Native" and the In Vitro-Obtained Apoprotein of Duck Serum HDL

Identification of Lipoprotein Families in High Density Lipoproteins of Normal Human Plasma

The Use of Streptolysin O as a Macromolecular Marker of Free Cholesterol in the β-Lipoprotein Molecule

Salting-In Gel Chromatographic Separation of Serum Low Density Lipoproteins on Sephadex Columns

Lipid Composition of Lipoprotein Fractions

Studies on the Proteins of Cat Lymph Chylomicrons

Role of Serum Lipoproteins in Hemolysis Induced by Vipera aspis Venom Phospholipases

Agarose Gel, Cellulose Acetate, and Polyacrylamide Gel Lipoprotein

Electrophoresis—A Comparison of Methods

Staining of Lipoproteins in Cellulose Acetate Membrane

Oxygenated Sterol Esters of Human Atherosclerotic Plaques

A2. Synthesis and Degradation

Comparative Studies on the In Vitro Effects of Delipidation with Organic Solvents, Enzymatic Lipolysis, Action of Anionic, Non-Ionic and Cationic Detergents and the In Vivo Effect of Lipoprotein-lipase on Duck Serum a-Lipoprotein

Study of the Human Serum β-Lipoprotein Antigenicity according to Its Degree of Delipidation

Influence of Cholesterol on the Concentration, Composition and Synthesis of Chicken Serum Lipoproteins

Isovaleric Acid in Acoustic Tissues of Porpoises: Triacylglycerols Resistant to Porcine Pancreatic Lipase

Effect of Clofibrate on Lipoprotein Metabolism

Biosynthesis of Plasma Glycolipoproteins

Human Intestinal Lipoprotein Particle Synthesis and Transport

The Metabolism of the Plasma Cholesterol in Man

Metabolism of Very Low Density Lipoproteins in Diabetes Mellitus

A3. Genetics

The Ag-System—Present Concepts and Immunogenetic Models

Serology and Genetics of the Ag-System

Genetic Polymorphisms of Lipoproteins

Immunological Relationship and Chemical Similarities between HDL Lipoproteins from Various Mammalian and Avian Species

On the Significance of the El (BA) Variants

Subunit Structure of the Lp(α) Lipoprotein

The Isolation and Partial Purification of Low Density Lipoproteins Carrying the Ag(x) and Ag(y) Antigenic Determinants

A Serum Type of Isoprecipitin System of HD Lipoproteins

Plasma Lipoprotein Distribution in Normal Children and Adults of Different Ages

A4. Function

The Arterial Foam Cell—In Vitro and In Vivo Studies

A New Method of Preparation for the Apoprotein Moiety from Folch-Pi Proteolipid

Evidence for a Covalent Bond between Lipid and a Membrane Protein (Rigid Layer of the Cell Wall of E. coli)

Distribution of Lipoproteins in Arteries and Tissue as Determined by Immunohistological and Radioactive Tracer Methods

Mechanism of Uptake of Plasma Lipoprotein Triglyceride by Avian Ovarian Follicles

Uptake of Plasma Lipoproteins by the Mammary Gland of Lactating Cows

Cholesterol Movements between Rat Plasmatic Lipoproteins and Red Cells In Vitro

The Formation of Lipoproteins in Hen Liver and Egg Yolk

A5. Pathology

Isolation and Properties of Abnormal Serum Lipoproteins in Cholestasis

Clinical Lipid Evaluations

Diagnosis of Familial Hypercholesterolaemia in Infancy

Dyslipidaemias in the Neonatal Period

Human Myeloma Proteins, which Bind β-Lipoproteins

Primary Hyperlipoproteinaemias in Myocardial Infarction

Type III Hyperlipoproteinaemia with Xanthomatosis

A Simple Method for the Detection and Identification of Various Types of Hyperlipoproteinemia

Problem Cases in Phenotyping

Phenotyping of Hyperlipidaemias by Electrochromatography

Lipid Electrophoresis on Cellulose Acetate Membranes and on Agarose

Quantitative Lipoprotein and Lipid Determinations in Patients with Various Malignancies

A General Review of Clinical and Laboratory Features of Familial Hypercholesterolemia (Type II Hyperbetalipoproteinemia)

A6. Dietary Measures and Drug Therapy The Validity of Animal Models in the Study of Atherogenesis

Dietary and Drug Management of Familial Hypercholesterolemia

Oxidative Metabolism in Normal and Hyperlipemic Patients

Management of Abetalipoproteinaemia in Childhood

Dietary and Hormonal Modifications of Rat Serum Lipoproteins

The Lipoproteins in Hyperlipidaemic Primates as a Model for Human Atherosclerosis

Section B: Hydroynamic Properties of Proteins

B1. Dielectrics

The Role of Dielectric Studies in the Investigation of Protein Molecules

The Dipole Moment of Urea and Thiourea in Water and Ethane Diol

Measurement of the Hydration of Biological Molecules from Dielectric Measurements at Centimeter Wavelengths

Recent Progress in the Dielectric Research on Polynucleotides

Permittivity Measurements on Biological Solutions at Very High Frequency

Deduction of Size and Hydration of Protein Molecules from Permittivity Measurements

B2. Fluorescence

The Use of Fluorescence Techniques for Studying the Structure and Structural Transitions of Polypeptides in Solution

The Decay of the Fluorescence Anisotropy of Biological Macromolecules

The Influence of the Microenvironments of the Tryptophan Groups of Proteins upon Their Fluorescence Properties

Determination of Fluorescence Lifetimes

Hydrodynamic Studies of Some Lysozymes and α-Lactalbumins

Section C: Protein Catabolism

Catabolism and Distribution of IgG in Different Diseases

Disorders of Immunoglobulin Catabolism

Thermodynamics of the Denaturation of Human Serum Albumin in Relation to Intra-vital Breakdown

Plasma Protein CataboHsm in the Capillary Wall?

IgM Degradation in Menetrier's Disease (Giant Hypertrophic Gastritis)

IgA Metabolism in Control Subjects, in Patients with Elevated IgA Levels and in Patients with IgA Deficiency

Possible Enhancement of IgM CataboHsm by D-Penicillamine

The CataboHsm of Human γG-Globulin and Its Fragments in Man and Mouse

A Consideration of Short-term Measurement of Plasma Protein Catabolic Rates

Rate of Synthesis of Albumin during the Early Postoperative Period

The Isolated Perfused Liver in the Study of Albumin Synthesis

Albumin Metabolism in Patients with Chronic Renal Failure on Low Protein Balanced Diet

Regulation of Transferrin Synthesis

CataboHsm of Nonmitochondrial Glutamic Oxalacetic Transaminase from Pig Heart in Piglets

The Turnover of Lysozyme

Section D: New Methods

Studies of Protein Interactions by Microcalorimetry

Isotachophoresis in Capillary Tubes

The Isolation of Human Transferrin by Isotachophoresis

Oil-Cooled Apparatus for Quantitative Immunoelectrophoresis

Quantitative Immunoelectrophoresis Applied to the Analysis of Elution Profiles in Column Chromatography

A Modified Radioimmunoassay for Quantitative Determination of IgG Subclasses in Man

Immunoselection Technique and Its Applications

Storage of Human Serum for Immunochemical Determination of the Serum Proteins

Enzyme-Linked Immunosorbent Assay

Determination of Insulin Binding to β-Lipoprotein in Sera from Normals and Diabetics Applying Immunoelectrophoresis

Candida Precipitins Characterized by a Modified Antigen-Antibody Crossed Electrophoresis An Automated System of Two-dimensional Immunoelectrophoresis

Micro-Polyacrylamide Flat Gel Electrophoresis: Advantages and Applications

Comparison of Slab and Cylinder Gel Focusing

Studies of Aggregates of IgG in Human Sera and Commercial γ-Globulin Preparations with a New Immuno-gel Filtration Method

Author Index

Subject Index


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About the Editor

H. Peeters

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