Protides of the Biological Fluids

Protides of the Biological Fluids

Proceedings of the Seventeenth Colloquium, Bruges, 1969

1st Edition - January 1, 1970

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  • Editor: H. Peeters
  • eBook ISBN: 9781483186542

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Protides of the Biological Fluids documents the proceedings of the XVIITH Colloquium held in Bruges in 1969. This book is divided into three sections. Section A discusses the genetics of the antibody response, focusing on the evolutionary aspects and genetics of differentiation centered on the problems of immunoglobulin structure and deficiencies. The molecular variation occurring in the amino acid sequence of various proteins, its ways of detection and its physiological significance, is presented in Section B. Some of the proteins considered include hemoglobin, fibrinogen, complement, and lipoproteins. Section C is devoted to the developments in theoretical and instrumental aspects of electrofocusing, considering the ampholyte structure and function and techniques, such as gel electrofocusing and preparative column electrofocusing. This compilation is beneficial to researchers and specialists concerned with protides of the biological fluids.

Table of Contents

  • Preface


    Academic Lecture

    Molecular Variation in Proteins

    Section A: Genetics of the Antibody Response

    Introduction to the Symposium

    A1. Evolutionary Aspects

    Evolution of the Immune Response

    Gene Duplication as an Evolutionary Force

    The Variability of Human Immunoglobulins

    The Genetic Control of Antibody Variability

    Specific Blocking of in vitro Antibody Response by Affinity Labeling Reagents

    The Inverse Wobble Theory

    Some Facts about the Evolution of Immunoglobulins

    A2. Genetics of Differentiation

    Thymus Deficiency in Mice

    Thymus and Possible Pathogenic Mechanisms of Immunologie Deficiency in Man

    The Genetics of IgA Deficiency

    Genetic Studies on Isolated Deficiency of γA Globulin

    The Deficiency of Certain Sub-Groups of Human γG as Demonstrated by the Study of Allotypes

    A3. Genetics of Productive Processes



    —Immunological Responsiveness to Haptens

    A4. Genetics of Regulatory Mechanisms

    Allotypy as a Probe

    The Expression of Immunoglobulin AUotypic Specificities by Rabbit Lymphocytes

    The Quantitative Expression, Genetics and Chemistry of Allotypes, Types and Subtypes of Rabbit Light Polypeptide Chains

    Antigenic Modulation

    Subclass and Allotype Differences in Human yG Immunoglobulins Reflected by the Amino Acid Sequence of Fc Glycopeptides

    The Genes of the Gm System, Are they Structural Genes?

    Section B: Molecular Variation

    B1. Haemoglobin

    Multiple α and ß Chain Structural Genes as a Basis for Hemoglobin Heterogeneity of the Adult Goat

    Investigations of Molecular Variation in Human Fetal Hemoglobin in the Infant and in Certain Hematological Conditions in the Adult

    Molecular Changes in Haemoglobins during Development and Their Functional Significance

    Duplication of the Haemoglobin α-chain Locus in Macaca irus

    On the Heterogeneity of Equine Myoglobin

    Partial Structure of Beef Myoglobin

    Unstable Haemoglobin with a Modified Affinity for Oxygen in a French Patient

    B2. Fibrinogen

    Molecular Variation of Human Fibrinogen

    Fibrinogen "Zurich". Separation of a Pathological from a Normal Fibrin Fraction

    Electrophoresis and Chromatography of Native and of Sulphitolysed Fibrinogen

    B3. Complement

    Genetic Polymorphism of Human C3

    C6-Deficiency in Rabbits

    Guinea Pig C'la-Inactivator: Purification and Characterization

    Characterization of the Purified Fourth Component of Guinea Pig Complement

    A Tool for the Detection of C'6 Deficiencies

    B4. Lipoproteins

    Location of Antigenic Specificity in Human Serum Lipoproteins

    The Explanation of the Variability in Streptolysin O Binding Capacity of Human β-Lipoprotein

    New Aspects of Human Low Density Lipoprotein Polymorphism

    Immunological and Biochemical Characterization of the LDL Proteins

    B5. Other Proteins

    Molecular Variations in Erythrocyte Catalase

    Molecular Variation in an Intra- and Extracellular R-type Vitamin B12-Binding Protein

    Heterogeneity of the Haptoglobin 1-1 Molecule by Electrophoresis of Human Serum in Starch Gels at pH 4-9

    Isolation of a Lysine-Rich, Serine-Rich Histone from Calf Thymus

    Section C: Electrofocusing

    C1. Instrumental

    Trends in Instrumental and Methodical Development of Isoelectric Focusing

    General Aspects of Isoelectric Focusing of Proteins

    Some Physical and Chemical Properties of the Ampholine Chemicals

    Preparative Isoelectric Focusing in a Sea-Serpent Shaped Apparatus

    Zone Convection Electrofocusing: A New Technique for Fractionation of Ampholytes in Free Solution

    Direct Optical Scanning of Isoelectric Fractionations

    A New Elution System for Isoelectric Focusing in Sucrose Gradients

    Protein Fractionation by Gel Electrofocusing: Importance of the Sample Application Procedure

    Some Applications of Gel Electrofocusing to the Separation and Characterization of Proteins

    Polyacrylamide Gel Electrophoresis after Isoelectric Focusing of Proteins

    Immunoglobulin Heterogeneity Studied by Isoelectric Focusing in Polyacrylamide Gel

    C2. Application

    Isoelectric Focusing and Immunological Characterization of Human Serum Albumin

    The Heterogeneity of Human Serum Albumin

    The Separation of Pooled Human IgG into Fractions by Isoelectric Focusing, and Their Electrophoretic and Immunological Properties

    The Application of Electrofocusing to the Fractionation of Proteins

    Studies on Haemolysins from Staphylococcus aureus by the Method of Isoelectric Focusing

    Separation and Characterization of Glucagon-Like Immunoactive Components from Gut Extracts by Electrofocusing

    Isoelectric Focusing of Lens Proteins

    Purification of Myeloma Proteins by Iso-electric Focusing

    The Isolation of the Fibrin Stabilizing Factor (FSF) from Human Plasma by Electrofocusing Techniques

    Isoelectric Focusing of the Steroid Binding β-globulin of Human Serum

    The Use of the Technique of Isoelectric Focusing in the Study of Certain Proteases

    Two Slow Bence-Jones K Monomers Separated by Isoelectric Focusing

    Section D: Techniques

    Evaluation of Molecular Radius by Exclusion Gel Electrophoresis, Demonstrated on Haptoglobins

    Preparation of Freeze-dried, Monomeric and Immunochemically Pure IgG by a Rapid and Reproducible Chromatographie Technique

    Gel Filtration of Heat Denatured Human Serum

    Effect of Different Agaroses and pH upon Agarose-gel Electrophoresis of Lipoproteins

    Molecular Parameters of Charged and Uncharged Macromolecules from Quantitative Thin-layer Electrophoresis in Sephadex Gels

    Changes in the β1c-β1A-Globulin During the Coagulation Process Demonstrated by Means of a Quantitative Immunoelectrophoresis Method

    Quantitative Estimation of Electrophoretically Heterogeneous Proteins by Electrophoresis in Antibody-containing Agarose Gel

    Author Index

    Subject Index

Product details

  • No. of pages: 558
  • Language: English
  • Copyright: © Pergamon 1970
  • Published: January 1, 1970
  • Imprint: Pergamon
  • eBook ISBN: 9781483186542

About the Editor

H. Peeters

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