Proteomics in Biomedicine and Pharmacology

Proteomics in Biomedicine and Pharmacology

1st Edition - June 24, 2014

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  • Editor: Rossen Donev
  • eBook ISBN: 9780128005958
  • Hardcover ISBN: 9780128004531

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Volume 95 of Advances in Protein Chemistry and Structural Biology focuses on advances in proteomic techniques and their application in biomedicine and pharmacology. This volume describes in detail the applications of these techniques in studying a number of diseases and specific protein modifications and profiles, including phosphoproteomics, glycoproteomics, and more. Published continuously since 1944, the Advances in Protein Chemistry and Structural Biology series is the essential resource for protein chemists. Each volume brings forth new information about protocols and analysis of proteins. Each thematically organized volume is guest edited by leading experts in a broad range of protein-related topics.

Key Features

  • Describes advances in application of powerful techniques in translational research (biomedicine and pharmacology)
  • Chapters are written by authorities in their field
  • Targeted to a wide audience of researchers, specialists, and students
  • The information provided in the volume is well supported by a number of high quality illustrations, figures, and tables


The intended audiences for this volume are researchers and specialists in biomedicine and pharmacology interested in using cutting edge proteomics strategies for discovery of suitable therapeutic targets and design of biologically active molecules. This volume would be also of a great use to students working on projects from the abovementioned areas.

Table of Contents

  • Contributors


    Chapter One. Endocrine-Disrupting Chemicals and Human Growth and Maturation: A Focus on Early Critical Windows of Exposure


    1 Introduction

    2 Challenges in Evidencing Endocrine Disruption

    3 Endocrine-Disrupting Chemicals and Fetal Growth

    4 EDCs and Sexual Differentiation

    5 EDCs and Puberty

    6 EDCs and Brain Development

    7 EDC and Energy Balance

    8 Epigenetic Perspective on the Developmental Effects of EDCs

    9 Conclusion


    Chapter Two. Agricultural Pesticides and Precocious Puberty


    1 Introduction

    2 Puberty

    3 Precocious Puberty

    4 The Effects of EDs on Puberty

    5 Organochlorine Pesticides and Puberty

    6 DDT and DDE

    7 Methoxychlor

    8 Endosulfans

    9 Vinclozolin

    10 Acetochlor

    11 Conclusion


    Chapter Three. Endocrine-Disrupting Chemicals: Elucidating Our Understanding of Their Role in Sex and Gender-Relevant End Points


    1 Introduction

    2 Endocrine Disruption May Underlie Negative Effects of Contaminants

    3 Adverse Health Consequences of Lifelong EDC Exposure

    4 Signficance of Investigating EDC Effects on Neurodevelopmental Processes

    5 Hormones’ Effects to Organize Neural Systems and Behavioral Processes

    6 Developmental EDC Exposure Alters Reproductive Development and Behavior

    7 Exposure to EDCs in Adulthood Also Effects Reproductive Parameters

    8 EDCs in Adulthood Influence Sexually Dimorphic Brain Morphology

    9 Do EDCs Influence Sex Differences in Nonreproductive Behaviors?

    10 Effects of EDCs on Sexually Dimorphic, Nonreproductive Behaviors

    11 Potential Mechanisms by which EDCs May Produce Their Effects

    12 Several Factors May Bear upon Estrogenicity of Compounds

    13 Effects of EDCs on Uterotropic Activity

    14 Importance of Examining EDC Effects on Whole-Animal Estrogenic Measures

    15 Effects of EDCs on E2 Metabolism

    16 Androgenic/Antiandrogenic Effects of EDCs

    17 Effects of EDCs via Traditional Intracellular Steroid Receptors

    18 Other Substrates to Consider for Actions of EDCs

    19 Preliminary Studies

    20 Organized Reproductive Parameters: Immature Rats

    21 Organized and Activated Reproductive Parameters: Peripubertal and Maturation Measures

    22 Organized and Activated Reproductive Parameters: Effects in Adults

    23 Strategy: EDC Effects on Reproductive Parameters: A Biomarker of Effects

    24 Organized and/or Activated Nonreproductive Sexually Dimorphic Behaviors

    25 Suggested Experiments Moving Forward

    26 Approach: The Importance of Integration of Reproductive and Nonreproductive Measures

    27 Approach: Examining Effects of EDC Exposure Throughout Development

    28 Suggestions for Future Work Examining Mechanisms of EDCs Effects

    29 Logistical Factors for Experimental Control and Power

    30 Summary

    31 Conclusions



    Chapter Four. Ovarian Toxicity from Reactive Oxygen Species


    1 Ovarian Follicular Development and Its Hormonal Regulation

    2 Reactive Oxygen Species and Oxidative Stress

    3 Hormonal Regulation of and Effects of Depleting or Enhancing Ovarian Antioxidants

    4 Oxidative Stress Occurs in the Ovarian Follicles When the Normal Hormonal Support for Follicular Survival is Removed

    5 Effects of Exogenous H2O2 on Ovarian Follicles and Granulosa Cells

    6 Involvement of ROS in Ovarian Toxicity Caused by Chemical Toxicants

    7 Involvement of ROS in Ovarian Toxicity Caused by Ionizing Radiation

    8 Summary and Conclusions


    Chapter Five. Low-Dose Effects of Hormones and Endocrine Disruptors


    1 Introduction

    2 What is Meant by “Low Dose?”

    3 Hormones Act at Low Doses

    4 The “Rules” of Hormones are Obeyed by EDCs: Implications for Low-Dose Chemical Exposures

    5 NTP Panel's Findings on Low-Dose Effects: 2000–2002

    6 The NTP Panel's Assessment: Low-Dose Effects of Specific Chemicals

    7 Updates to the NTP Panel Report on Low-Dose Effects

    8 BPA and the Prostate: A Low-Dose Case Study

    9 Additional Low-Dose Findings: Evidence from Laboratory Animals and Human Populations

    10 Traditional Toxicology Studies are Not Designed to Detect Low-Dose Effects

    11 Low-Dose Effects: Biological Changes are Not Necessarily Adverse

    12 Real World EDC Exposures: Mixture Effects

    13 Nonmonotonic Dose Responses

    14 Conclusions


    Chapter Six. Contamination from Endocrine Disrupters of the General Population at Low and High Concentrations


    1 Introduction

    2 How to Implement the Indicator? An Example

    3 Results of the Implementation of the Indicator

    4 Discussion


    Chapter Seven. Reproductive Toxicities of Methoxychlor Based on Estrogenic Properties of the Compound and Its Estrogenic Metabolite, Hydroxyphenyltrichloroethane


    1 Introduction

    2 Estrogenicity of Methoxychlor and Its Active Metabolite, HPTE

    3 Reproductive and Developmental Toxicities of Methoxychlor

    4 Conclusion



    Chapter Eight. Epigenetic Effects of Methoxychlor and Vinclozolin on Male Gametes


    1 Introduction

    2 Epigenetics

    3 Imprinting

    4 Environmental Changes Affect Imprinting

    5 Imprinting Defects and Male Infertility

    6 Endocrine Disruptors and Male Infertility

    7 Conclusion and Perspectives


    Chapter Nine. Nuclear Receptor Profiling of Bisphenol-A and Its Halogenated Analogues


    1 Introduction

    2 Estrogen Receptors

    3 Estrogen-Related Receptor γ

    4 Androgen Receptor

    5 Peroxysome Proliferator-Activated Receptor γ

    6 Thyroid Receptors

    7 Pregnane X Receptor

    8 Conclusions



    Chapter Ten. Long-Lasting Effects of Neonatal Bisphenol A Exposure on the Implantation Process


    1 Introduction

    2 Uterine Implantation of the Embryo

    3 Endocrine Disruptors and the Developmental Programming Hypothesis

    4 Endocrine Disruption and Female Fertility

    5 Conclusions



    Chapter Eleven. The Obesogen Tributyltin


    1 The Obesogen Hypothesis

    2 Organotins Environmental Exposure

    3 Mechanisms of Cellular Organotin Toxicity

    4 Nuclear Hormone Receptors as Organotin Targets

    5 TBT and Disrupted Adipose Biology

    6 Epigenetic Perturbations in Adipogenesis and Metabolic Set Points

    7 Conclusion and Perspective



    Chapter Twelve. Human Testicular Insulin-Like Factor 3 and Endocrine Disrupters


    1 Introduction

    2 INSL3: Expression and Regulation

    3 Functions of INSL3

    4 INSL3 and Endocrine Disrupters

    5 Conclusions and Future Directions


    Chapter Thirteen. Environmental Pollutants and Hydroxysteroid Dehydrogenases


    1 Introduction

    2 3β-Hydroxysteroid Dehydrogenase/Isomerase

    3 11β-Hydroxysteroid Dehydrogenase

    4 17β-Hydroxysteroid Dehydrogenase

    5 20α-Hydroxysteroid Dehydrogenase

    6 Summary and Conclusion



    Chapter Fourteen. Cadmium Effects on the Thyroid Gland


    1 Introduction

    2 Cadmium

    3 Structural and Functional Organization of the Thyroid Gland

    4 Cadmium and the Thyroid Gland

    5 Molecular Mechanism of Cadmium Influence

    6 Conclusions and Future Directions


    Chapter Fifteen. A Recombinant PPRE-Driven Luciferase Bioassay for Identification of Potential PPAR Agonists


    1 Introduction

    2 Materials

    3 Methods

    4 Notes




Product details

  • No. of pages: 376
  • Language: English
  • Copyright: © Academic Press 2014
  • Published: June 24, 2014
  • Imprint: Academic Press
  • eBook ISBN: 9780128005958
  • Hardcover ISBN: 9780128004531

About the Serial Editor

Rossen Donev

Rossen Donev
Rossen Donev received his PhD degree in 1999 from the Institute of Molecular Biology, Bulgarian Academy of Sciences. He did postdoctoral training at Imperial Cancer Research Fund, UK (renamed after the merger with Cancer Research Campaign to Cancer Research UK, London Research Institute) and Cardiff University. In 2007 he was awarded a New Investigator Grant Award from the Medical Research Council (UK) to establish himself as an independent Principle Investigator. In 2010 Dr. Donev was appointed Senior Lecturer at Swansea University. In 2016 Dr. Donev joined MicroPharm Ltd (UK) where currently he is Head of Research. He has published more than 60 research papers, chaired scientific meetings and given invited plenary talks. Rossen Donev has consulted on projects related to development of treatments for neurodevelopmental disorders and cancer therapies. He serves as Editor-in-Chief of the Advances in Protein Chemistry and Structural Biology and on editorial board of several other journals. His research interests include signaling pathways involved in neuropsychiatric disorders and tumor escape from the immune system, and development of therapeutic strategies for their treatment. More recently he has focused on development of immunotherapeutics for non-systemic applications.

Affiliations and Expertise

Head of Research, MicroPharm Limited, UK

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