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Proteomics in Biomedicine and Pharmacology - 1st Edition - ISBN: 9780128004531, 9780128005958

Proteomics in Biomedicine and Pharmacology, Volume 95

1st Edition

Serial Editor: Rossen Donev
Hardcover ISBN: 9780128004531
eBook ISBN: 9780128005958
Imprint: Academic Press
Published Date: 24th June 2014
Page Count: 376
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Table of Contents



Chapter One. Endocrine-Disrupting Chemicals and Human Growth and Maturation: A Focus on Early Critical Windows of Exposure


1 Introduction

2 Challenges in Evidencing Endocrine Disruption

3 Endocrine-Disrupting Chemicals and Fetal Growth

4 EDCs and Sexual Differentiation

5 EDCs and Puberty

6 EDCs and Brain Development

7 EDC and Energy Balance

8 Epigenetic Perspective on the Developmental Effects of EDCs

9 Conclusion


Chapter Two. Agricultural Pesticides and Precocious Puberty


1 Introduction

2 Puberty

3 Precocious Puberty

4 The Effects of EDs on Puberty

5 Organochlorine Pesticides and Puberty

6 DDT and DDE

7 Methoxychlor

8 Endosulfans

9 Vinclozolin

10 Acetochlor

11 Conclusion


Chapter Three. Endocrine-Disrupting Chemicals: Elucidating Our Understanding of Their Role in Sex and Gender-Relevant End Points


1 Introduction

2 Endocrine Disruption May Underlie Negative Effects of Contaminants

3 Adverse Health Consequences of Lifelong EDC Exposure

4 Signficance of Investigating EDC Effects on Neurodevelopmental Processes

5 Hormones’ Effects to Organize Neural Systems and Behavioral Processes

6 Developmental EDC Exposure Alters Reproductive Development and Behavior

7 Exposure to EDCs in Adulthood Also Effects Reproductive Parameters

8 EDCs in Adulthood Influence Sexually Dimorphic Brain Morphology

9 Do EDCs Influence Sex Differences in Nonreproductive Behaviors?

10 Effects of EDCs on Sexually Dimorphic, Nonreproductive Behaviors

11 Potential Mechanisms by which EDCs May Produce Their Effects

12 Several Factors May Bear upon Estrogenicity of Compounds

13 Effects of EDCs on Uterotropic Activity

14 Importance of Examining EDC Effects on Whole-Animal Estrogenic Measures

15 Effects of EDCs on E2 Metabolism

16 Androgenic/Antiandrogenic Effects of EDCs

17 Effects of EDCs via Traditional Intracellular Steroid Receptors

18 Other Substrates to Consider for Actions of EDCs

19 Preliminary Studies

20 Organized Reproductive Parameters: Immature Rats

21 Organized and Activated Reproductive Parameters: Peripubertal and Maturation Measures

22 Organized and Activated Reproductive Parameters: Effects in Adults

23 Strategy: EDC Effects on Reproductive Parameters: A Biomarker of Effects

24 Organized and/or Activated Nonreproductive Sexually Dimorphic Behaviors

25 Suggested Experiments Moving Forward

26 Approach: The Importance of Integration of Reproductive and Nonreproductive Measures

27 Approach: Examining Effects of EDC Exposure Throughout Development

28 Suggestions for Future Work Examining Mechanisms of EDCs Effects

29 Logistical Factors for Experimental Control and Power

30 Summary

31 Conclusions



Chapter Four. Ovarian Toxicity from Reactive Oxygen Species


1 Ovarian Follicular Development and Its Hormonal Regulation

2 Reactive Oxygen Species and Oxidative Stress

3 Hormonal Regulation of and Effects of Depleting or Enhancing Ovarian Antioxidants

4 Oxidative Stress Occurs in the Ovarian Follicles When the Normal Hormonal Support for Follicular Survival is Removed

5 Effects of Exogenous H2O2 on Ovarian Follicles and Granulosa Cells

6 Involvement of ROS in Ovarian Toxicity Caused by Chemical Toxicants

7 Involvement of ROS in Ovarian Toxicity Caused by Ionizing Radiation

8 Summary and Conclusions


Chapter Five. Low-Dose Effects of Hormones and Endocrine Disruptors


1 Introduction

2 What is Meant by “Low Dose?”

3 Hormones Act at Low Doses

4 The “Rules” of Hormones are Obeyed by EDCs: Implications for Low-Dose Chemical Exposures

5 NTP Panel's Findings on Low-Dose Effects: 2000–2002

6 The NTP Panel's Assessment: Low-Dose Effects of Specific Chemicals

7 Updates to the NTP Panel Report on Low-Dose Effects

8 BPA and the Prostate: A Low-Dose Case Study

9 Additional Low-Dose Findings: Evidence from Laboratory Animals and Human Populations

10 Traditional Toxicology Studies are Not Designed to Detect Low-Dose Effects

11 Low-Dose Effects: Biological Changes are Not Necessarily Adverse

12 Real World EDC Exposures: Mixture Effects

13 Nonmonotonic Dose Responses

14 Conclusions


Chapter Six. Contamination from Endocrine Disrupters of the General Population at Low and High Concentrations


1 Introduction

2 How to Implement the Indicator? An Example

3 Results of the Implementation of the Indicator

4 Discussion


Chapter Seven. Reproductive Toxicities of Methoxychlor Based on Estrogenic Properties of the Compound and Its Estrogenic Metabolite, Hydroxyphenyltrichloroethane


1 Introduction

2 Estrogenicity of Methoxychlor and Its Active Metabolite, HPTE

3 Reproductive and Developmental Toxicities of Methoxychlor

4 Conclusion



Chapter Eight. Epigenetic Effects of Methoxychlor and Vinclozolin on Male Gametes


1 Introduction

2 Epigenetics

3 Imprinting

4 Environmental Changes Affect Imprinting

5 Imprinting Defects and Male Infertility

6 Endocrine Disruptors and Male Infertility

7 Conclusion and Perspectives


Chapter Nine. Nuclear Receptor Profiling of Bisphenol-A and Its Halogenated Analogues


1 Introduction

2 Estrogen Receptors

3 Estrogen-Related Receptor γ

4 Androgen Receptor

5 Peroxysome Proliferator-Activated Receptor γ

6 Thyroid Receptors

7 Pregnane X Receptor

8 Conclusions



Chapter Ten. Long-Lasting Effects of Neonatal Bisphenol A Exposure on the Implantation Process


1 Introduction

2 Uterine Implantation of the Embryo

3 Endocrine Disruptors and the Developmental Programming Hypothesis

4 Endocrine Disruption and Female Fertility

5 Conclusions



Chapter Eleven. The Obesogen Tributyltin


1 The Obesogen Hypothesis

2 Organotins Environmental Exposure

3 Mechanisms of Cellular Organotin Toxicity

4 Nuclear Hormone Receptors as Organotin Targets

5 TBT and Disrupted Adipose Biology

6 Epigenetic Perturbations in Adipogenesis and Metabolic Set Points

7 Conclusion and Perspective



Chapter Twelve. Human Testicular Insulin-Like Factor 3 and Endocrine Disrupters


1 Introduction

2 INSL3: Expression and Regulation

3 Functions of INSL3

4 INSL3 and Endocrine Disrupters

5 Conclusions and Future Directions


Chapter Thirteen. Environmental Pollutants and Hydroxysteroid Dehydrogenases


1 Introduction

2 3β-Hydroxysteroid Dehydrogenase/Isomerase

3 11β-Hydroxysteroid Dehydrogenase

4 17β-Hydroxysteroid Dehydrogenase

5 20α-Hydroxysteroid Dehydrogenase

6 Summary and Conclusion



Chapter Fourteen. Cadmium Effects on the Thyroid Gland


1 Introduction

2 Cadmium

3 Structural and Functional Organization of the Thyroid Gland

4 Cadmium and the Thyroid Gland

5 Molecular Mechanism of Cadmium Influence

6 Conclusions and Future Directions


Chapter Fifteen. A Recombinant PPRE-Driven Luciferase Bioassay for Identification of Potential PPAR Agonists


1 Introduction

2 Materials

3 Methods

4 Notes





Volume 95 of Advances in Protein Chemistry and Structural Biology focuses on advances in proteomic techniques and their application in biomedicine and pharmacology. This volume describes in detail the applications of these techniques in studying a number of diseases and specific protein modifications and profiles, including phosphoproteomics, glycoproteomics, and more.

Published continuously since 1944, the Advances in Protein Chemistry and Structural Biology series is the essential resource for protein chemists. Each volume brings forth new information about protocols and analysis of proteins. Each thematically organized volume is guest edited by leading experts in a broad range of protein-related topics.

Key Features

  • Describes advances in application of powerful techniques in translational research (biomedicine and pharmacology)
  • Chapters are written by authorities in their field
  • Targeted to a wide audience of researchers, specialists, and students
  • The information provided in the volume is well supported by a number of high quality illustrations, figures, and tables


The intended audiences for this volume are researchers and specialists in biomedicine and pharmacology interested in using cutting edge proteomics strategies for discovery of suitable therapeutic targets and design of biologically active molecules. This volume would be also of a great use to students working on projects from the abovementioned areas.


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© Academic Press 2014
24th June 2014
Academic Press
Hardcover ISBN:
eBook ISBN:

Ratings and Reviews

About the Serial Editor

Rossen Donev

Rossen Donev

Rossen Donev received his PhD degree in 1999 from the Institute of Molecular Biology, Bulgarian Academy of Sciences. He did postdoctoral training at Imperial Cancer Research Fund, UK (renamed after the merger with Cancer Research Campaign to Cancer Research UK, London Research Institute) and Cardiff University. In 2007 he was awarded a New Investigator Grant Award from the Medical Research Council (UK) to establish himself as an independent Principle Investigator. In 2010 Dr. Donev was appointed Senior Lecturer at Swansea University. In 2016 Dr. Donev joined MicroPharm Ltd (UK) where currently he is Head of Research. He has published more than 60 research papers, chaired scientific meetings and given invited plenary talks. Rossen Donev has consulted on projects related to development of treatments for neurodevelopmental disorders and cancer therapies. He serves as Editor-in-Chief of the Advances in Protein Chemistry and Structural Biology and on editorial board of several other journals. His research interests include signaling pathways involved in neuropsychiatric disorders and tumor escape from the immune system, and development of therapeutic strategies for their treatment. More recently he has focused on development of immunotherapeutics for non-systemic applications.

Affiliations and Expertise

Swansea University, UK