Proteomic and Metabolomic Approaches to Biomarker Discovery

1st Edition

Editors: Haleem J Issaq Timothy D. Veenstra
Hardcover ISBN: 9780123944467
eBook ISBN: 9780123947956
Imprint: Academic Press
Published Date: 18th June 2013
Page Count: 488
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Proteomic and Metabolomic Approaches to Biomarker Discovery demonstrates how to leverage biomarkers to improve accuracy and reduce errors in research. Disease biomarker discovery is one of the most vibrant and important areas of research today, as the identification of reliable biomarkers has an enormous impact on disease diagnosis, selection of treatment regimens, and therapeutic monitoring. Various techniques are used in the biomarker discovery process, including techniques used in proteomics, the study of the proteins that make up an organism, and metabolomics, the study of chemical fingerprints created from cellular processes.

Proteomic and Metabolomic Approaches to Biomarker Discovery is the only publication that covers techniques from both proteomics and metabolomics and includes all steps involved in biomarker discovery, from study design to study execution.  The book describes methods, and presents a standard operating procedure for sample selection, preparation, and storage, as well as data analysis and modeling. This new standard effectively eliminates the differing methodologies used in studies and creates a unified approach. Readers will learn the advantages and disadvantages of the various techniques discussed, as well as potential difficulties inherent to all steps in the biomarker discovery process.

A vital resource for biochemists, biologists, analytical chemists, bioanalytical chemists, clinical and medical technicians, researchers in pharmaceuticals, and graduate students, Proteomic and Metabolomic Approaches to Biomarker Discovery provides the information needed to reduce clinical error in the execution of research.

Key Features

  • Describes the use of biomarkers to reduce clinical errors in research
  • Includes techniques from a range of biomarker discoveries
  • Covers all steps involved in biomarker discovery, from study design to study execution


Biochemists, analytical chemists, bioanalytical chemists, biologists, medical technicians, clinical technicians, researchers in pharmaceuticals, graduate students and all interested in the field of biomarkers research.

Table of Contents


List of Contributors

Chapter 1. Biomarker Discovery: Study Design and Execution




The Current State of Biomarker Discovery

Study Design and Execution

Errors in Study Design

Errors in Study Execution

Specificity of Proteins as Biomarkers

Statistical Data Analysis


Concluding Remarks and Recommendations


Chapter 2. Proteomic and Mass Spectrometry Technologies for Biomarker Discovery

Nonstandard Abbreviations


Protein Biomarker Discovery and Development Pipeline

Proteomic Samples

Protein Identification by Mass Spectrometry

Post-Translational Modifications as Disease Biomarkers

Protein Quantification by Mass Spectrometry

Biomarker Verification

Biomarker Validation

Limitations of Mass Spectrometry for Protein Biomarker Discovery

Conclusions and Future Outlook: Integrated Biomarker Discovery Platform


Chapter 3. Tissue Sample Preparation for Proteomic Analysis


Types of Tissues Available for MS-Based Proteomics

Tissue Disruption/Homogenization

Extraction/Solubilization Buffers

Concluding Remarks


Chapter 4. Sample Preparation in Global Metabolomics of Biological Fluids and Tissues



An Ideal Sample Preparation Method for Global Metabolomics?

Sample Preparation Methods for Biofluids

Tissue Metabolomics

New Trends in Sample Preparation for Global Metabolomics

Conclusions and Future Perspective


Chapter 5. Serum and Plasma Collection: Preanalytical Variables and Standard Operating Procedures in Biomarker Research


Importance of Prean


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© Academic Press 2013
Academic Press
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About the Editor

Haleem J Issaq

Affiliations and Expertise

Ph.D., Laboratory of Proteomics and Analytical Technologies, SAIC/Frederick National Cancer Institute at Frederick, Frederick, MD, USA.

Timothy D. Veenstra

Affiliations and Expertise

Ph.D., Mass Spectrometry Center and Laboratory of Proteomics and Analytical Technologies, SAIC/Frederick National Cancer Institute at Frederick, Frederick, MD, USA.


"The book is primarily aimed at researchers with significant expertise in proteomic or metabolomic technologies with an interest in pursuing biomarker discovery studies. The chapters are well written, informative, and provide insight into various steps from sample acquisition to data analysis. The book is an excellent starting point for anyone interested in this field."--Analytical and Bioanalytical Chemistry (2014) 406
"Researchers in various medical and biological specialties focus mostly on preparing samples for proteomic and metabolomic studies, rather than on using the imaging equipment."--Reference and Research Book News, August 2013