- Print ISBN 9780123944467
- Electronic ISBN 9780123947956
Proteomic and Metabolomic Approaches to Biomarker Discovery demonstrates how to leverage biomarkers to improve accuracy and reduce errors in research. Disease biomarker discovery is one of the most vibrant and important areas of research today, as the identification of reliable biomarkers has an enormous impact on disease diagnosis, selection of treatment regimens, and therapeutic monitoring. Various techniques are used in the biomarker discovery process, including techniques used in proteomics, the study of the proteins that make up an organism, and metabolomics, the study of chemical fingerprints created from cellular processes.
Proteomic and Metabolomic Approaches to Biomarker Discovery is the only publication that covers techniques from both proteomics and metabolomics and includes all steps involved in biomarker discovery, from study design to study execution. The book describes methods, and presents a standard operating procedure for sample selection, preparation, and storage, as well as data analysis and modeling. This new standard effectively eliminates the differing methodologies used in studies and creates a unified approach. Readers will learn the advantages and disadvantages of the various techniques discussed, as well as potential difficulties inherent to all steps in the biomarker discovery process.
A vital resource for biochemists, biologists, analytical chemists, bioanalytical chemists, clinical and medical technicians, researchers in pharmaceuticals, and graduate students, Proteomic and Metabolomic Approaches to Biomarker Discovery provides the information needed to reduce clinical error in the execution of research.
Biochemists, analytical chemists, bioanalytical chemists, biologists, medical technicians, clinical technicians, researchers in pharmaceuticals, graduate students and all interested in the field of biomarkers research.
List of Contributors
Chapter 1. Biomarker Discovery: Study Design and Execution
The Current State of Biomarker Discovery
Study Design and Execution
Errors in Study Design
Errors in Study Execution
Specificity of Proteins as Biomarkers
Statistical Data Analysis
Concluding Remarks and Recommendations
Chapter 2. Proteomic and Mass Spectrometry Technologies for Biomarker Discovery
Protein Biomarker Discovery and Development Pipeline
Protein Identification by Mass Spectrometry
Post-Translational Modifications as Disease Biomarkers
Protein Quantification by Mass Spectrometry
Limitations of Mass Spectrometry for Protein Biomarker Discovery
Conclusions and Future Outlook: Integrated Biomarker Discovery Platform
Chapter 3. Tissue Sample Preparation for Proteomic Analysis
Types of Tissues Available for MS-Based Proteomics
Chapter 4. Sample Preparation in Global Metabolomics of Biological Fluids and Tissues
An Ideal Sample Preparation Method for Global Metabolomics?
Sample Preparation Methods for Biofluids
New Trends in Sample Preparation for Global Metabolomics
Conclusions and Future Perspective
Chapter 5. Serum and Plasma Collection: Preanalytical Variables and Standard Operating Procedures in Biomarker Research
Importance of Prean
"The book is primarily aimed at researchers with significant expertise in proteomic or metabolomic technologies with an interest in pursuing biomarker discovery studies. The chapters are well written, informative, and provide insight into various steps from sample acquisition to data analysis. The book is an excellent starting point for anyone interested in this field."--Analytical and Bioanalytical Chemistry (2014) 406