The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Key Features

@introbul:Key Features @bul:* Presents new ideas on classification of proteolytic enzymes * Covers 100 individual proteolytic enzymes * Offers numerous medical implications of work in this area * Provides uses of these enzymes in biotechnology


Biochemists, molecular biologists, cell biologists, microbiologists, physiologists, neuroscientists, and immunologists.

Table of Contents

A.J. Barrett, Classification of Peptidases. Serine Peptidases: N.D. Rawlings and A.J. Barrett, Families of Serine Peptidases. J.R. Hoidal, N.V. Rao, and B. Gray, Myeloblastin: Leukocyte Proteinase 3. M.M. Simon and M.D. Kramer, Granzyme A. M.C. Peitsch and J. Tschopp, Granzyme B. L.B. Schwartz, Tryptase: A Mast Cell Serine Protease. K. Kurachi, A. Torres-Rosado, and A. Tsuji, Hepsin. J.J. Birktoft and K. Breddam, Glutamyl Endopeptidases. F. Sakiyama and T. Masaki, Lysyl Endopeptidase of Achromobacter lyticus. A.G. Plaut and W.W. Bachovchin, IgA-Specific Prolyl Endopeptidases: Serine Type. C. Brenner, A. Bevan, and R.S. Fuller, Biochemical and Genetic Methods for Analyzing Specificity and Activity of Precursor-Processing Enzyme: Yeast Kex2 Protease, Kexin. K. Nakayama, Purification of Recombinant Soluble Forms of Furin Produced in Chinese Hamster Ovary Cells. N.G. Seidah and M. Chretien, Pro-Protein Convertases of Subtilisin/Kexin Family. L. Polgar, Prolyl Oligopeptidases. D. Tsuru and T. Yoshimoto, Oligopeptidase B: Protease II from Escherichia coli. Y. Ikehara, S. Ogata, and Y. Misumi, Dipeptidyl-peptidase IV from Rat Liver. W.M. Jones, A. Scaloni, and J.M. Manning, Acylaminoacyl-peptidase. S.J. Remington and K. Breddam, Carboxypeptidases C and D. B. Granier, M. Jamin, M. Adam, M. Galleni, B. Lakaye, W. Zorzi, J. Grandchamps, J.-M. Wilkin, C. Fraipont, B. Joris, C. Duez, M. Nguyen-Distoche, J. Coyette, M. Leyh-Bouille, J. Dusart, L. Christiaens, J.-M. Frore, and J.-M. Ghuysen, Serine-Type D-Ala-D-Ala Peptidases and Penic


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© 1994
Academic Press
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@from:Praise for the Volume @qu:"Indeed, the volume encompasses not only preparation, assay, and function, but also a remarkably rich lode of information on the underlying biology of the tissues, microorganisms, and viruses that are the sources of the peptidasesunder discussion... This volume is a welcome and valuable addition to the biochemist's library." @source:--ANALYTICAL BIOCHEMISTRY @from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY