In this volume of Methods in Enzymology and its companion Volume 244, the chapters on specific methods, enzymes, and inhibitors are organized within the rational framework of the new systems for classificationand nomenclature. A wide variety of specificities of peptide bond hydrolysis are represented in each set of peptidases, together with an equally wide range of biological functions.

Key Features

@introbul:Key Features @bul:* Aspartic peptidases * Metallopeptidases * New information on classification of proteolytic enzymes * Medical implications of research in this area * Biotechnological uses of these enzymes


Biochemists, molecular biologists, cell biologists, microbiologists, physiologists, neuroscientists, and immunologists.

Table of Contents

Methods: J.C. Powers and C.-M. Kam, Peptide Thioester Substrates for Serine Peptidases and Metalloendopeptidases. C.G. Knight, Fluorimetric Assays of Proteolytic Enzymes. G.M. McGeehan, D.M. Bickett, J.S. Wiseman, M. Green, and J. Berman, Defined Substrate Mixtures for Mapping of Proteinase Specificities. C.J. Handley and D.J. Buttle, Assay of Proteoglycan Degradation. J.G. Bieth, Theoretical and Practical Aspects of Proteinase Inhibition Kinetics. C.G. Knight, Active-Site Titration of Peptidases. Aspartic Peptidases: N.D. Rawlings and A.J. Barrett, Families of Aspartic Peptidases, and Those of Unknown Catalytic Mechanism. T. Kageyama, Procathepsin E and Cathepsin E. Y.P. Loh and N.X. Cawley, Processing Enzymes of Pepsin Family: Yeast Aspartic Protease 3 and Pro-opiomelanocortin Converting Enzyme. K. Takahashi, Proteinase A from Aspergillus niger. X. Lin and J. Tang, Thermopsin. K. Sankaran and H.C. Wu, Bacterial Prolipoprotein Signal Peptidase. Metallopeptidases: N.D. Rawlings and A.J. Barrett, Evolutionary Families of Metallopeptidases. D.S. Auld, Removal and Replacement of Metal Ions in Metallopeptidases. K. Morihara, Pseudolysin and Other Pathogen Endopeptidases of Thermolysin Family. C. Li and L.B. Hersh, Neprilysin: Assay Methods, Purification, and Characterization. B.P. Roques, F. Noble, P. Crine, and M.-C. Fournie-Zaluski, Inhibitors of Neprilysin: Design, Pharmacological and Clinical Applications. P. Corvol, T.A. Williams, and F. Soubrier, Peptidyl Dipeptidase A: Angiotensin I-Convert


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© 1995
Academic Press
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