Proteins are of fundamental importance in all aspects of cell structure and function. The study of proteins has always formed a central part of biochemistry, and recent expansion in the range and sophistication of available techniques has provided a wealth of new information. The current methods and approaches used to gain a better understanding of the structure of proteins are described within this book, while other articles focus on the role of proteins within the cell. Most of the articles have appeared previously in the monthly review journal Trends in Biochemical Sciences (TIBS), with a few commissioned specifically for this collection, which should appeal to students, lecturers and researchers interested in the form and function of proteins.

Table of Contents

Protein Primary Structure. Highlights of protein structural analysis (J.E. Shively, R.J. Pacton and T.D. Lee). Micro-level protein and peptide separations (K.J. Wilson). Site-directed mutagenesis and protein engineering (M. Smith). From analysis to synthesis: new ligand binding sites on the lactate dehydrogenase framework (A.R. Clarke, T. Atkinson and J.J. Holbrook). Subtilisin: an enzyme designed to be engineered (J.A. Wells and D.A. Estell). &Bgr;-galactoside transport in E. coli: functional dissection of lac permease (H.R. Kaback, E. Bibi and P.D. Roepe). Similar amino acid sequences revisited (R.F. Doolittle). Protein Conformation. Protein crystallagraphy: more surprises ahead (D. Eisenberg and C. Hill). Catalysis in enzyme crystals (J. Hadju et al). What can two-dimensional NMRT tell us about proteins? (P.E. Wright). Chiroptical methods in protein analysis (G. Snatzke). Protein conformational prediction (G.D. Fasman). Membrane protein models: possibilities and probabilities (M. Engelman and J. Popot). From comparisons of protein sequences and structures to protein modelling and design (A. Sali, J.P. Overington, M.S. Johnson and T.L. Blundell). Protein motifs and data-base searching (J.M. Thornton and S.P. Gardner). The de novo design of protein structures (J.S. Richardson and D.C. Richardson). Conformational stability of globular proteins (C.N. Pace). How does protein folding get started? (R.L. Baldwin). Co- and Post-Translational Modifications. Protein translocation and turnover in eukaryotic cells (R.A. Bradshaw). Post-translational modification of proteins: some problems left to solve (S.C.B. Yan, B.W. Grinnell and F. Wold). Proteolytic processing and physiological regulation (H. Neurath). Glycoproteins: what are the sugar chains for? (J.C. Paulson). Nuclear and cytoplasmic glycosylation: novel saccharide linkages in unexpected places (G.W. Hart


No. of pages:
© 1990
eBook ISBN:
Print ISBN:

About the editors