Proteins and Nucleic Acids

Proteins and Nucleic Acids

1st Edition - January 1, 1973

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  • Editor: Isidore Gersh
  • eBook ISBN: 9780323151504

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Description

Submicroscopic Cytochemistry, Volume I: Proteins and Nucleic Acids presents laboratory findings and theoretical aspects involved or derived from submicroscopic cytochemistry study of proteins and nucleic acids. It is a two-volume book, encompassing 14 chapters covering three major topics: the pattern of distribution of nucleic acids at the molecular level in various cells and states of activity and in relation to protein synthesis; the molecular and macromolecular organization of cellular membranes; and the origin and distribution of the major macromolecular aggregates of connective tissue. After a brief introduction to the aspects of submicroscopic cytochemistry, the book discusses morphology, with emphasis on the distribution of cell components and their organization in cells at the macromolecular level. It then presents developed methods to preserve and selectively identify proteins, nucleic acids, lipids, acid mucopolysaccharides, and molecular aggregates very nearly in the position they occupied in the living state. Chapters 8 to 12 describe DNA molecular patterns during cell differentiation and cell maturation, and the alteration of these patterns in certain mouse cells. A discussion on DNA molecular patterns during metaplasia of stratified squamous mouse epithelium and of spinal ganglion cells during regeneration is provided. The concluding chapters examine DNA organization during mitotic cycle in Drosophila eggs and the persistence of chromosome individuality during interphase. This book is a valuable source of information for cytochemists, cell biologists, cell researchers, and protein nucleic acid enthusiasts who wish to expand their knowledge on comprehensive cytochemistry laboratory study.

Table of Contents


  • List of Contributors

    Preface

    Acknowledgments

    Contents of Volume II

    Introduction

    1. Submicroscopic Cytochemistry

    Fixation

    Staining

    Other Preparatory Steps

    Consideration of Artifacts of Freeze-Drying and Freeze-Substitution

    Identification of Submicroscopic Structure in Cytochemical Electron Micrographs

    Summary

    References

    2. Distribution of Protein in Hepatic and Exocrine Pancreatic Cells of the Mouse

    Methods

    Observations

    Discussion

    Summary

    References

    3. Methods for the Fixation and Staining of DNA and RNAs Applied to Exocrine Pancreatic and Hepatic Cells of the Mouse

    Method

    Observations

    Discussion

    Summary

    Appendix

    References

    4. Cytochemical Study of Nucleic Acids in Salivary Gland Chromosomes of Drosophila Larvae

    Materials and Methods

    Observations

    Discussion

    Summary

    References

    5. Distribution of Protein in Nuclei of Salivary Glands of Drosophila Larvae

    Methods

    Observations

    Discussion

    Summary

    References

    6. DNA Pattern s of Cells of Imaginal Disks of Drosophila Larvae

    Method

    Discussion

    Summary

    References

    7. Submicroscopic Distribution of Nucleic Acids in the Barr Body of Chondrocytes in the Proximal Tibial Epiphysis of Female Rats

    Material and Methods

    Observations

    Discussion

    Summary

    References

    8. Preliminary Study of the Specificity of the DNA Molecular Patterns of Differentiated Cells of the Young Adult Mouse

    Method and Materials

    General Observations

    Discussion

    Summary

    References

    9. Changing DNA Molecular Patterns during Cell Maturation in Method and Material

    Observations

    Discussion

    Summary

    References

    10. Alteration of DNA Molecular Pattern s with Activation of Certain Cells in the Mouse

    Methods

    Observations

    Discussion

    Summary

    References

    11. DNA Molecular Pattern during Metaplasia of Stratified Squamous Epithelium of Mouse Vagina to Mucin-Producing Cells

    Materials and Method

    Observations

    Discussion

    Summary

    References

    12. Changes in Nuclear DNA Molecular Pattern of Spinal Ganglion Cells during Regeneration of Spinal Nerves of the Cat

    Method

    Observations

    Discussion

    Summary

    References

    13. Organization of DNA during the Mitotic Cycle in Eggs of Drosophila melanogaster

    Methods

    Observations

    Discussion

    Summary

    References

    14. Persistence of Individuality of Chromosomes during Interphase, and the Role of the Nuclear Membrane

    Transformation of Interphase Chromatin to Prophase Chromosomes, and of Telophase Chromosomes to Interphase Chromatin, in Living Cells

    Distribution of DNA in Chromatin in Unstimulated Somatic Cells

    Distribution of DNA during the Change of Interphase Chromatin to Prophase

    Chromosomes and of Telophase Chromosome to Interphase Chromatin

    Changes in Distribution of DNA in Chromatin of Activated Cells

    Changes in Distribution of DNA in Somatic Cell Nuclei during Differentiation and Maturation

    Persistence of Specific Orientation of Chromosomes during Metaphase

    Summary

    References

    Author Index

    Subject Index


Product details

  • No. of pages: 412
  • Language: English
  • Copyright: © Academic Press 1973
  • Published: January 1, 1973
  • Imprint: Academic Press
  • eBook ISBN: 9780323151504

About the Editor

Isidore Gersh

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