Protein Transfer and Organelle Biogenesis

Protein Transfer and Organelle Biogenesis

1st Edition - May 28, 1988

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  • Editor: Rathindra C. Das
  • eBook ISBN: 9780323142168

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Protein Transfer and Organelle Biogenesis is a seven-section volume focusing on the property of proteins to carry signals for secretion, mitochondrial assembly, and lysosomal localizations. It describes how these signals function and determines other factors necessary in building and maintaining the functions of a cell. Organized into seven sections encompassing 19 chapters, the book deals with the general aspects of protein translocation, modification, and sorting. It discusses the transport into the endoplasmic reticulum, the role of carbohydrates in glycoprotein trafficking, and the mechanism of endo- and exocytosis. It further discusses the localization of proteins to the mitochondrion and nucleus and with bacterial protein transport. The final section emphasizes the contribution secretion research that the biotechnology industry has made to the production of proteins. Biologists, cell biologists, researchers, teachers, and students who are interested in the mechanism of protein transfer and organelle biogenesis will find this book invaluable.

Table of Contents

  • Contributors


    Part I. Translocation

    1. Transport of Proteins into and across the Endoplasmic Reticulum Membrane

    I. Introduction

    II. Historical Background

    III. Targeting

    IV. Mechanism of Translocation

    V. Membrane Assembly of Integral Transmembrane Proteins

    VI. Overview


    Part II. Modification, Maturation, and Transport

    2. Role of Carbohydrate in Glycoprotein Traffic and Secretion

    I. Introduction

    II. Evidence for Intracellular Transport Signals

    III. Oligosaccharide Biosynthesis

    IV. Role of Carbohydrate in Protein Solubility, Structure, and Stability

    V. Evidence for Carbohydrate Transport Signals Using Site-Directed Mutagenesis

    VI. Mutations That Alter Glycosylation and Glycoprotein Traffic

    VII. Tunicamycin and Glycoprotein Traffic

    VIII. Inhibitors of Oligosaccharide Processing and Glycoprotein Traffic

    IX. Vertebrate Lectins and Glycoprotein Traffic

    X. Concluding Remarks


    3. Membrane Insertion and Transport of Viral Glycoproteins: A Mutational Analysis

    I. Introduction

    II. Genetic Approaches to Viral Glycoprotein Transport

    III. Conclusions


    4. Posttranslational Modification during Protein Secretion

    I. Introduction

    II. Reactions in the Cytoplasm

    III. Reactions in the Endoplasmic Reticulum

    IV. Protein Modification in the Golgi Apparatus

    V. Conclusion


    5. Mannosidases in Mammalian Glycoprotein Processing

    I. Introduction

    II. Processing Mannosidases and Multiple Routes

    III. Conclusions


    Part III. Sorting and Organelle Assembly

    6. Biosynthesis and Sorting of Proteins of the Endoplasmic Reticulum

    I. Introduction

    II. Structure and Sorting of ERp99, an Abundant, Conserved ER Glycoprotein

    III. Conclusions and Future Directions


    7. Functional Topology of Golgi Membranes

    I. Introduction

    II. General Properties of the Golgi Apparatus

    III. Topology of Membrane-Bound Functions

    IV. Transport Properties of Golgi Membranes

    V. Concluding Remarks


    8. Protein Sorting and Biogenesis of the Lysosome-like Vacuole in Yeast

    I. Introduction

    II. Biosynthesis of Vacuolar Proteins

    III. Sorting of Vacuolar Proteins

    IV. Endocytosis in Yeast and Its Relationship to Vacuolar Protein Delivery

    V. Summary and Conclusions


    9. Transport and Targeting of Lysosomal Enzymes in Dictyostelium discoideum

    I. Introduction

    II. Life History of a Cellular Slime Mold Lysosomal Enzyme

    III. Dual Pathways for the Secretion of Lysosomal Enzymes

    IV. Genetic Approaches to Dissect Intracellular Transport Pathways

    V. Developmental Regulation of the Synthesis, Modification, and Localization of Lysosomal Enzymes

    VI. Recombinant DNA Approaches

    VII. Summary and Future Perspectives


    10. Organelles of Endocytosis and Exocytosis

    I. Introduction

    II. Endocytosis

    III. Exocytosis

    IV Summary and Future Prospects


    11. Endocytosis and Compartmentalization of Lysosomal Enzymes in Normal and Mutant Mammalian Cells: Mannose 6-Phosphate-Dependent Pathways

    I. In the Beginning

    II. The Recognition Marker

    III. The Receptors

    IV. Endocytosis

    V. The Intracellular Pathway

    VI. Junction of the Pathways


    12. Biogenesis of Secretory Vesicles

    I. Introduction

    II. Structure and Function of Secretory Organelles

    III. Formation of Secretory Vesicles

    IV Conclusion and Perspectives


    13. Expression of Extracellular Matrixlike Glycoproteins by Macrophages and Other Leukocytes

    I. Introduction

    II. Biosynthesis of Extracellular Matrixlike Glycoproteins by Macrophages

    III. Functions of Extracellular Matrixlike Glycoproteins in Macrophages and Related Cells

    IV. Perspectives


    Part IV. Transfer Across Bacterial Membranes

    14. Synthesis and Export of Lipoproteins in Bacteria

    I. Introduction

    II. Li pop rote in Structure

    III. Biosynthesis

    IV Distributions of Lipoproteins among Bacteria

    V Signal Peptidases

    VI. Genomic Organization of the Isp Gene

    VII. Prolipoprotein Structural Gene Mutants Affecting Modification and/or Processing

    VIII. Early Steps in Lipoprotein Secretion

    IX. Concluding Remarks


    15. Protein Secretion across the Outer Membrane of Gram-Negative Bacteria

    I. Introduction

    II. Lysis or Secretion

    III. Early Stages in the Secretion Pathway

    IV. Later Stages in the Secretion Pathway: Secretion Proteins and Targeting Signals

    V. Release of Proteins in Vesicles

    VI. Activators and Inhibitors of Secreted Proteins

    VII. Special Cases

    VIII. Concluding Remarks and Perspectives


    Part V. Mitochondrial Assembly

    16. Genetic Approaches to the Study of Mitochondrial Protein Import

    I. Introduction

    II. Isolation of Mutations affecting Mitochondrial Import

    III. Conclusions


    17. Synthesis and Assembly of Mitrochondrial Proteins

    I. Introduction

    II. Precursor Proteins

    III. Targeting and Sorting Sequences

    IV. Receptors

    V. Energy Requirements

    VI. Cytosolic Cofactors

    VII. Translocation Contact Sites

    VIII. Proteolytic Processing

    IX. Mitochondrial Gene Products

    X. Assembly and Coordination

    XI. Overview


    Part VI. Nuclear Transport

    18. Transport of Proteins into the Nucleus

    I. Introduction

    II. Nuclear Pore

    III. Possible Mechanisms for Specific Nuclear Protein Localization

    IV. Nuclear Protein Localization in the Yeast Saccharomyces cerevisiae

    V. Conclusion


    Part VII. Applied Research

    19. Secretion Research in Industrial Mycology

    I. Introduction

    II. Commercially Important Enzymes of Fungal Origin

    III. Secretion of Therapeutic Mammalian Polypeptides in Saccharomyces cervisiae

    IV. Applied Secretion Research: Prospects for the Future



Product details

  • No. of pages: 866
  • Language: English
  • Copyright: © Academic Press 1988
  • Published: May 28, 1988
  • Imprint: Academic Press
  • eBook ISBN: 9780323142168

About the Editor

Rathindra C. Das

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