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Biomolecular Interactions Part A
1st Edition, Volume 166 - October 27, 2021
Editor: Arun K. Shukla
Language: English
Hardback ISBN:9780128233511
9 7 8 - 0 - 1 2 - 8 2 3 3 5 1 - 1
eBook ISBN:9780128233528
9 7 8 - 0 - 1 2 - 8 2 3 3 5 2 - 8
Biomolecular Interactions: Part A, Volume 166, the latest release in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting in…Read more
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Biomolecular Interactions: Part A, Volume 166, the latest release in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics in cell biology. Each chapter is written by an international board of authors.
Provides the authority and expertise of leading contributors from an international board of authors
Presents the latest release in the Methods in Cell Biology series
Updated release includes the latest information on biomolecular interactions instead of protein-protein interactions
Academic, government and industrial sectors
Cover image
Title page
Table of Contents
Copyright
Contributors
Preface
Chapter 1: Measuring the rapid kinetics of receptor-ligand interactions in live cells using NanoBRET
Abstract
1: Introduction
2: Materials
3: Methods
4: Notes
Acknowledgments
References
Chapter 2: Evaluating functional ligand-GPCR interactions in cell-based assays
Abstract
1: Introduction
2: Overview of the protocol
3: Step-by-step methods
4: Limitations
5: Summary
Acknowledgments
References
Chapter 3: Assays for detecting arrestin interaction with GPCRs
Abstract
1: Introduction
2: Direct binding assay between purified arrestin and rhodopsin
3: Brief overview of other assays using purified components
4: Overview of common cell-based arrestin recruitment assays
5: Cell-based arrestin recruitment assays to unmodified GPCRS
6: Summary
7: Key resources table
Acknowledgments
References
Chapter 4: BRET-based assay to specifically monitor β2AR/GRK2 interaction and β-arrestin2 conformational change upon βAR stimulation
Abstract
1: Introduction
2: Materials
3: Methods
4: Notes
References
Chapter 5: Cannabinoid receptor CB1 and CB2 interacting proteins: Techniques, progress and perspectives
Abstract
1: Introduction
2: Canonical G protein signaling interactions
3: Non-G protein signaling mediators and modulators
4: Receptor oligomerization
5: Interactions influencing subcellular distribution
6: Putative interactors with as yet undefined function
7: Perspectives and future vistas to expand the cannabinoid receptor interactome
References
Chapter 6: Purinergic GPCR transmembrane residues involved in ligand recognition and dimerization
Abstract
1: Introduction
2: AR and P2YR ligands and structures
3: Analysis of small molecule recognition by TM residues of adenosine receptors
4: Analysis of small molecule recognition by TM residues of P2Y receptors
5: Receptor domains involved in dimerization
6: Summary
Acknowledgment
References
Chapter 7: Nanobodies as sensors of GPCR activation and signaling
Abstract
1: Introduction
2: Nanobodies as emerging tools to study GPCR activation and signaling
3: Examples of using nanobodies to probe KOR activation
4: Step-by-step protocols
5: Data analysis
6: Conclusion
References
Chapter 8: Confocal and TIRF microscopy based approaches to visualize arrestin trafficking in living cells
Abstract
1: Introduction
2: Arrestins roles in GPCR trafficking and signaling
3: β-arrestin trafficking to the PM
4: β-arrestin actions from the PM
5: β-arrestin actions from endocytic compartments
6: Significance of arrestin trafficking
7: Overview of the protocols
8: Step-by-step methods
9: Additional methods
10: Summary
Acknowledgments
References
Chapter 9: Strategies for targeting cell surface proteins using multivalent conjugates and chemical biology
Abstract
1: Introduction
2: Discussion
3: Conclusions and future directions
Acknowledgments
References
Chapter 10: Identifying Plasmodium falciparum receptor activation using bioluminescence resonance energy transfer (BRET)-based biosensors in HEK293 cells
Chapter 11: Methods for binding analysis of small GTP-binding proteins with their effectors
Abstract
1: Introduction
2: Yeast two-hybrid (Y2H) assay
3: Co-immunoprecipitation (co-IP)
4: Notes
References
Chapter 12: Investigating protein expression, modifications and interactions in the brain: Protocol for preparing rodent brain tissue for mass spectrometry-based quantitative- and phospho-proteomics analysis
Abstract
1: Introduction
2: Mass spectrometry-based proteomics
3: Protein-protein interactions in neurodegenerative disorders
4: Overview of the protocol
5: Step-by-step protocol
6: Summary
Acknowledgments
References
Chapter 13: Protein-protein interactions at a glance: Protocols for the visualization of biomolecular interactions
Abstract
1: Introduction
2: Protein-protein interaction network
3: Protein structures and protein complexes
4: Protein-protein interface: shape and chemical complementarity
5: Protein complexes in motion
6: Photorealistic representations of protein complexes
7: Protein-protein interactions: hot spots and small molecule design
8: Selectivity of protein interactions
9: Summary and outlook
Acknowledgments
References
Chapter 14: Interactions between noncoding RNAs as epigenetic regulatory mechanisms in cardiovascular diseases
Abstract
1: Introduction
2: Molecular functions of the different classes of regulatory ncRNAs
3: Interactions between the different types of noncoding RNAs in cardiovascular diseases
4: Perspectives and therapeutic applications of noncoding RNAs in CVDs
5: Conclusion
Competing interest
References
No. of pages: 368
Language: English
Edition: 1
Volume: 166
Published: October 27, 2021
Imprint: Academic Press
Hardback ISBN: 9780128233511
eBook ISBN: 9780128233528
AS
Arun K. Shukla
Dr. Arun K. Shukla is a world leader in the field of GPCR biology and he is currently a Professor in the Department of Biological Sciences and Bioengineering at the Institute of Technology, Kanpur in India. Dr. Shukla’s research program is focused on understanding the structure, function and regulation of G protein-coupled receptors with a long-term of designing novel therapeutics with minimized side-effects.
Affiliations and expertise
Indian Institute of Technology, Kanpur, India
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