Protein Prenylation, Part A - 1st Edition - ISBN: 9780123813398, 9780123813404

Protein Prenylation, Part A, Volume 29

1st Edition

Serial Volume Editors: Christine Hrycyna Martin Bergo Fuyuhiko Tamanoi
eBook ISBN: 9780123813404
Hardcover ISBN: 9780123813398
Imprint: Academic Press
Published Date: 22nd July 2011
Page Count: 356
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Table of Contents


Protein Prenylation

I. Abstract

II. Steps in the Trail of Research

Insights into the Function of Prenylation from Nuclear Lamin Farnesylation

I. Abstract

II. Introduction

III. Subcellullar Trafficking and Processing of Prelamin A

IV. Step 2 Endoprotease Activity and Zmpste24

V. Functional Aspects of Lamin Farnesylation

VI. Relationship of Lamin Prenylation to the Functional Role of Prenylation in Other Proteins

Posttranslational Processing of Nuclear Lamins

I. Abstract

II. Introduction

III. B-Type Lamins

IV. A-Type Lamins

V. Defects in Prelamin A Processing and Disease

VI. Alternate Prenylation of Prelamin A

VII. The Purpose of Prelamin A Processing

VIII. Concluding Thoughts


Prenylated Proteins in Peroxisome Biogenesis

I. Abstract

II. Peroxisomes

III. Biogenesis of Peroxisomes

Lipid Modification of Ras Superfamily GTPases

I. Abstract

II. Introduction

III. Ras Superfamily Prenyltransferases

IV. Ras Family Members

V. Rho Family Members

VI. Rab Family Members

VII. The Arf Family of Small GTPases

VIII. The Ran Small GTPase

IX. Prenyltransferases as Therapeutic Targets

X. Conclusion


Heterogeneous Prenyl Processing of the Heterotrimeric G protein Gamma Subunits

I. Abstract

II. Heterotrimeric G Proteins and Prenylation of the Gγ Subunit

III. Functional Role of G Protein γ Subunit Prenylation

IV. Variation in Prenyl Processing of Brain G Proteins

V. Conclusion

Farnesylation Versus Geranylgeranylation in G-Protein-Mediated Light Signaling

I. Abstract

II. Introduction

III. Characteristic Properties of Phototransduction in Retinal Rod Cells

IV. Particular Lipidation of Photoreceptor Proteins

V. Biochemical Differences Between Farnesylation and Geranylgeranylation

VI. Physiological Significance of Farnesylation of Gtγ

VII. Biological Significance and Conclusion

Organization and Function of the Rab Prenylation and Recycling Machinery

I. Abstract

II. Rab GTPases

III. Pathways of Rab Prenylation

IV. Organization of the Rab Prenylation Complex and Mechanism of the Prenylation Reaction

V. Structure of the Rab:GDI Complex and Its Functional Segregation from Rab:REP Complex

VI. Extraction of Rab Proteins from Membranes by GDI and REP

VII. Targeting of Rabs to Specific Membranes

VIII. Rab Prenylation in Disease

IX. Conclusions

Protein Prenylation CaaX Processing in Plants

I. Abstract

II. The Plant Protein Prenyltransferase Enzymes

III. FT and GGT-I Substrates in Plants

IV. Protein Prenylation—A Crossroad Between Signaling and Metabolism

V. Prenyltransferase Mutants in Plants

VI. CaaX Processing

VII. Summary


Posttranslational Isoprenylation of Tryptophan Residues in Bacillus subtilis

I. Abstract

II. Quorum Sensing in Bacteria

III. Genetic Competence in B. subtilis

IV. Primary Gene Cluster for ComX Pheromone Production

V. Posttranslational Modification of ComX Pheromone

VI. Structure–Activity Relationships of ComXRO-E-2 Pheromone

VII. Is Posttranslational Isoprenylation of Tryptophan Universal?

VIII. Summary and Future Prospects


Global Analysis of Prenylated Proteins by the Use of a Tagging via Substrate Approach

I. Abstract

II. Introduction

III. General Approach: Tagging via Substrate Approach Utilizing Azide Chemistry

IV. Detection of Farnesylated Proteins

V. Detection of Geranylgeranylated Proteins

VI. Overall Profiles of Farnesylated and Geranylgeranylated Proteins and Further Improvements

VII. Applications of the TAS Methods

VIII. Other Tagging Methods and Toward Constructing Prenylome


Global Identification of Protein Prenyltransferase Substrates

I. Abstract

II. Introduction

III. Methods for Discovering and Predicting Prenyltransferase Substrates

IV. Prenylation in Pathogenic Organisms: Structural and Biochemical Insights

V. Conclusions


Structural Biochemistry of CaaX Protein Prenyltransferases

I. Abstract

II. Protein Farnesyltransferase Structure and Reaction Cycle

III. Protein Geranylgeranyltransferase-I Structure and Reaction Cycle

IV. Determinants of Ca1a2X Substrate Selection in FTase and GGTase-I

V. Structure of Candida albicans Protein Geranylgeranyltransferase-I

VI. Inhibitors of Protein Prenyltransferases as Cancer Chemotherapeutics

VII. FTase Inhibitors for Treatment of Malaria and Other Infectious Diseases

Genetic Analyses of the CAAX Protein Prenyltransferases in Mice

I. Abstract

II. Introduction

III. The Prenyltransferases FTase and GGTase-I

IV. FTase

V. GGTase-I

VI. Simultaneous Inactivation of FTase and GGTase-I

VII. Concluding Remarks

Farnesyl Transferase Inhibitors

I. Abstract

II. Targeting Ras with Farnesyl Transferase Inhibitors

III. Discovery of Farnesyl Transferase Inhibitors

IV. Activity of FTIs in Preclinical Cancer Models and Alternative Prenylation of Ras

V. Impact of FTIs on Other Farnesylated Proteins

VI. Clinical Studies of FTIs in Cancer

VII. Hutchinson–Gilford Progeria Syndrome: A Disease of Farnesylation?

VIII. Conclusions and Other Therapeutic Opportunities for FTIs



This volume of The Enzymes features high-caliber thematic articles on the topic of glycosylphosphatidylinositol (GPI) anchoring of proteins.

Key Features

  • Contributions from leading authorities
  • Informs and updates on all the latest developments in the field


Biochemists, cell biologists, molecular biologists, biophysicists


No. of pages:
© Academic Press 2011
Academic Press
eBook ISBN:
Hardcover ISBN:

About the Serial Volume Editors

Christine Hrycyna Serial Volume Editor

Affiliations and Expertise

Purdue University, West Lafayette, Indiana

Martin Bergo Serial Volume Editor

Affiliations and Expertise

Wallenberg Laboratory, Institute of Medicine, Sahlgrenska Hospital, Gothenburg, Sweden

Fuyuhiko Tamanoi Serial Volume Editor

Fuyu Tamanoi is a biochemist who has served on the UCLA School of Medicine and UCLA College faculty since he joined the Department of Microbiology, Immunology & Molecular Genetics in 1993. He became a full professor in 1997. Since 1996, he has been a Director of Signal Transduction Program Area at Jonsson Comprehensive Cancer Center. Dr. Tamanoi earned his B.S. and M.S. in Biochemistry at the University of Tokyo. He received PhD in Molecular Biology at Nagoya University in 1977. He was a postdoctoral fellow at Harvard Medical School, where he worked on bacteriophage DNA replication. From 1980 to 1985, he was a senior staff investigator at Cold Spring Harbor Laboratory, where he worked on adenovirus DNA replication. From 1985 to 1993, he was an Assistant Professor and then Associate Professor at the University of Chicago, where he initiated studies on lipid modification of the Ras family proteins. His laboratory research centers on signal transduction and signal transduction inhibitors. He is currently exploring ways to deliver signal transduction inhibitors using nanoparticles.

Affiliations and Expertise

Professor and Vice Chair, Dept. of Microbiology, Immunology & Molecular Genetics, University of California, Los Angeles, USA Director, Signal Transduction Program Area, Jonsson Comprehensive Cancer Center, USA