Protein Phosphorylation, Part A, Volume 200

1st Edition

Protein Kinases: Assays, Purification, Antibodies, Functional Analysis, Cloning, and Expression

Serial Volume Editors: Tony Hunter Bartholomew Sefton
Editor-in-Chiefs: John Abelson Melvin Simon
Hardcover ISBN: 9780121821012
eBook ISBN: 9780080883151
Imprint: Academic Press
Published Date: 28th July 1991
Page Count: 763


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Table of Contents

Classification of Protein Kinases and Phosphorylation Site Sequences:

T. Hunter, Protein Kinase Classification.

S.K. Hanks and A.M. Quinn, Protein Kinase Catalytic Domain Sequence Database: Identification of Conserved Features of Primary Structure and Classification of Family Members.

R.B. Pearson and B.E. Kemp, Protein Kinase Phosphorylation Site Sequences and Consensus Specificity Motifs: Tabulations.

Assays of Protein Kinases:

C.V.C. Glover and C.D. Allis, Enzyme Activity Dot Blots for Assaying Protein Kinases.

S.-L. Li, D. Sahal, and Y. Fujita-Yamaguchi, Solid-Phase Protein-Tyrosine Kinase Assays.

G. Rijksen, B.A. van Oirschit, and G.E.J. Staal, Nonradioactive Assays of Protein-Tyrosine Kinase Activity Using Antiphosphotyrosine Antibodies.

E. Racker, Use of Synthetic Amino Acid Polymers for Assay of Protein-Tyrosine and Protein-Serine Kinases.

E. Racker and P.C. Sen, Assay of Phosphorylation of Small Substrates and of Synthetic Random Polymers That Interact Chemically with Adenosine 5prime-Triphosphate.

J.E. Casnellie, Assay of Protein Kinases Using Peptides with Basic Residues for Phosphocellulose Binding.

B.E. Kemp and R.B. Pearson, Design and Use of Peptide Substrates for Protein Kinases.

D.R. Marshak and D. Carroll, Synthetic Peptide Substrates for Casein Kinase II. Purification of Protein Kinases: General Methods:

S. Ferrari and G. Thomas, Micro and Macro Purification Methods for Protein Kinases.

J.R. Woodgett, Use of Synthetic Peptides Mimicking Phosphorylation Sites for Affinity Purification of Protein-Serine Kinases.

P. Jeno and G. Thomas, Affinity Purification of Protein Kinases Using Adenosine 5prime-Tri


This volume provides a compilation of recent methods for studying protein phosphorylation.


Biochemists, cell biologists, molecular biologists, developmental biologists, geneticists, and biophysicists.


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© Academic Press 1991
Academic Press
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About the Serial Volume Editors

Tony Hunter Serial Volume Editor

Tony Hunter received his Ph.D. in 1969 from the University of Cambridge, England. He joined the Salk Institute in 1975 as an assistant professor and has been a professor since 1982. His current interests are the protein-tyrosine kinases of the Src and growth factor receptor families and the protein-tyrosine phosphatases that remove the phosphates added by protein-tyrosine kinases. He was elected as a Fellow of the Royal Society of London in 1987, a Fellow of the American Academy of Arts and Sciences in 1992, and as an Associate Member of the European Molecular Biology Organization in 1992.

Affiliations and Expertise

The Salk Institute, La Jolla, California, USA

Bartholomew Sefton Serial Volume Editor

Affiliations and Expertise

The Salk Institute, San Diego, California, U.S.A.

About the Editor-in-Chiefs

John Abelson Editor-in-Chief

Affiliations and Expertise

California Institute of Technology, Division of Biology, Pasadena, U.S.A.

Melvin Simon Editor-in-Chief

Affiliations and Expertise

The Salk Institute, La Jolla, CA, USA