Volume 200. Protein Phosphorylation, Part A

1st Edition

Protein Kinases: Assays, Purification, Antibodies, Functional Analysis, Cloning, and Expression

Serial Volume Editors:

Description

This volume provides a compilation of recent methods for studying protein phosphorylation.

Readership

Biochemists, cell biologists, molecular biologists, developmental biologists, geneticists, and biophysicists.

Table of Contents

Classification of Protein Kinases and Phosphorylation Site Sequences: T. Hunter, Protein Kinase Classification. S.K. Hanks and A.M. Quinn, Protein Kinase Catalytic Domain Sequence Database: Identification of Conserved Features of Primary Structure and Classification of Family Members. R.B. Pearson and B.E. Kemp, Protein Kinase Phosphorylation Site Sequences and Consensus Specificity Motifs: Tabulations. Assays of Protein Kinases: C.V.C. Glover and C.D. Allis, Enzyme Activity Dot Blots for Assaying Protein Kinases. S.-L. Li, D. Sahal, and Y. Fujita-Yamaguchi, Solid-Phase Protein-Tyrosine Kinase Assays. G. Rijksen, B.A. van Oirschit, and G.E.J. Staal, Nonradioactive Assays of Protein-Tyrosine Kinase Activity Using Antiphosphotyrosine Antibodies. E. Racker, Use of Synthetic Amino Acid Polymers for Assay of Protein-Tyrosine and Protein-Serine Kinases. E. Racker and P.C. Sen, Assay of Phosphorylation of Small Substrates and of Synthetic Random Polymers That Interact Chemically with Adenosine 5prime-Triphosphate. J.E. Casnellie, Assay of Protein Kinases Using Peptides with Basic Residues for Phosphocellulose Binding. B.E. Kemp and R.B. Pearson, Design and Use of Peptide Substrates for Protein Kinases. D.R. Marshak and D. Carroll, Synthetic Peptide Substrates for Casein Kinase II. Purification of Protein Kinases: General Methods: S. Ferrari and G. Thomas, Micro and Macro Purification Methods for Protein Kinases. J.R. Woodgett, Use of Synthetic Peptides Mimicking Phosphorylation Sites for Affinity Purification of Protein-Serine Kinases. P. Jeno and G. Thomas, Affinity Purification of Protein Kinases Using Adenosine 5prime-Tri

Details

No. of pages:
763
Language:
English
Copyright:
© 1991
Published:
Imprint:
Academic Press
eBook ISBN:
9780080883151
Print ISBN:
9780121821012

About the serial-volume-editors

Tony Hunter

Tony Hunter received his Ph.D. in 1969 from the University of Cambridge, England. He joined the Salk Institute in 1975 as an assistant professor and has been a professor since 1982. His current interests are the protein-tyrosine kinases of the Src and growth factor receptor families and the protein-tyrosine phosphatases that remove the phosphates added by protein-tyrosine kinases. He was elected as a Fellow of the Royal Society of London in 1987, a Fellow of the American Academy of Arts and Sciences in 1992, and as an Associate Member of the European Molecular Biology Organization in 1992.

Affiliations and Expertise

The Salk Institute, La Jolla, California, USA

Bartholomew Sefton

Affiliations and Expertise

The Salk Institute, San Diego, California, U.S.A.

Reviews

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