Protein Phosphorylation, Part A, Volume 200

1st Edition

Protein Kinases: Assays, Purification, Antibodies, Functional Analysis, Cloning, and Expression

Print ISBN: 9780121821012
eBook ISBN: 9780080883151
Imprint: Academic Press
Published Date: 28th July 1991
Page Count: 763


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This volume provides a compilation of recent methods for studying protein phosphorylation.


Biochemists, cell biologists, molecular biologists, developmental biologists, geneticists, and biophysicists.

Table of Contents

Classification of Protein Kinases and Phosphorylation Site Sequences:

T. Hunter, Protein Kinase Classification.

S.K. Hanks and A.M. Quinn, Protein Kinase Catalytic Domain Sequence Database: Identification of Conserved Features of Primary Structure and Classification of Family Members.

R.B. Pearson and B.E. Kemp, Protein Kinase Phosphorylation Site Sequences and Consensus Specificity Motifs: Tabulations.

Assays of Protein Kinases:

C.V.C. Glover and C.D. Allis, Enzyme Activity Dot Blots for Assaying Protein Kinases.

S.-L. Li, D. Sahal, and Y. Fujita-Yamaguchi, Solid-Phase Protein-Tyrosine Kinase Assays.

G. Rijksen, B.A. van Oirschit, and G.E.J. Staal, Nonradioactive Assays of Protein-Tyrosine Kinase Activity Using Antiphosphotyrosine Antibodies.

E. Racker, Use of Synthetic Amino Acid Polymers for Assay of Protein-Tyrosine and Protein-Serine Kinases.

E. Racker and P.C. Sen, Assay of Phosphorylation of Small Substrates and of Synthetic Random Polymers That Interact Chemically with Adenosine 5prime-Triphosphate.

J.E. Casnellie, Assay of Protein Kinases Using Peptides with Basic Residues for Phosphocellulose Binding.

B.E. Kemp and R.B. Pearson, Design and Use of Peptide Substrates for Protein Kinases.

D.R. Marshak and D. Carroll, Synthetic Peptide Substrates for Casein Kinase II. Purification of Protein Kinases: General Methods:

S. Ferrari and G. Thomas, Micro and Macro Purification Methods for Protein Kinases.

J.R. Woodgett, Use of Synthetic Peptides Mimicking Phosphorylation Sites for Affinity Purification of Protein-Serine Kinases.

P. Jeno and G. Thomas, Affinity Purification of Protein Kinases Using Adenosine 5prime-Tri


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