Protein NMR Spectroscopy
Principles and Practice
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Protein NMR Spectroscopy, Second Edition combines a comprehensive theoretical treatment of NMR spectroscopy with an extensive exposition of the experimental techniques applicable to proteins and other biological macromolecules in solution. Beginning with simple theoretical models and experimental techniques, the book develops the complete repertoire of theoretical principles and experimental techniques necessary for understanding and implementing the most sophisticated NMR experiments. Important new techniques and applications of NMR spectroscopy have emerged since the first edition of this extremely successful book was published in 1996. This updated version includes new sections describing measurement and use of residual dipolar coupling constants for structure determination, TROSY and deuterium labeling for application to large macromolecules, and experimental techniques for characterizing conformational dynamics. In addition, the treatments of instrumentation and signal acquisition, field gradients, multidimensional spectroscopy, and structure calculation are updated and enhanced. The book is written as a graduate-level textbook and will be of interest to biochemists, chemists, biophysicists, and structural biologists who utilize NMR spectroscopy or wish to understand the latest developments in this field.
Key Features
- Provides an understanding of the theoretical principles important for biological NMR spectroscopy
- Demonstrates how to implement, optimize and troubleshoot modern multi-dimensional NMR experiments
- Allows for the capability of designing effective experimental protocols for investigations of protein structures and dynamics
- Includes a comprehensive set of example NMR spectra of ubiquitin provides a reference for validation of experimental methods
Readership
Graduate students, postdoctoral fellows, established researchers interested in structural biology
Table of Contents
PREFACE
PREFACE TO THE FIRST EDITION
ACKNOWLEDGEMENTS
Chapter 1: CLASSICAL NMR SPECTROSCOPY
Chapter 2: THEORETICAL DESCRIPTION OF NMR SPECTROSCOPY
Chapter 3: EXPERIMENTAL ASPECTS OF NMR SPECTROSCOPY
Chapter 4: MULTIDIMENSIONAL NMR SPECTROSCOPY
Chapter 5: RELAXATION AND DYNAMIC PROCESSES
Chapter 6: EXPERIMENTAL 1H NMR METHODS
Chapter 7: HETERONUCLEAR NMR EXPERIMENTS
Chapter 8: EXPERIMENTAL NMR RELAXATION METHODS
Chapter 9: LARGER PROTEINS AND MOLECULAR INTERACTIONS
Chapter 10: SEQUENTIAL ASSIGNMENT, STRUCTURE DETERMINATION, AND OTHER APPLICATIONS
TABLE OF SYMBOLS
LIST OF FIGURES
LIST OF TABLES
SUGGESTED READING
INDEX
SPIN-1/2 PRODUCT OPERATOR EQUATIONS
TABLE OF CONSTANTS
Product details
- No. of pages: 912
- Language: English
- Copyright: © Academic Press 2006
- Published: November 20, 2006
- Imprint: Academic Press
- Hardcover ISBN: 9780121644918
- eBook ISBN: 9780080471037
About the Authors
John Cavanagh
Dr. Cavanagh is the William Neal Reynolds Distinguished Professor of Biochemistry at North Carolina State University. He is an expert in protein structural biology, particularly in how bacteria are able to protect themselves. Dr. Cavanagh received his Ph.D. in Chemistry/NMR spectroscopy from the University of Cambridge in 1988. He has held positions as a Senior Research Associate at The Scripps Research Institute, Director of Structural Biology at the Wadsworth Center (New York State Department of Health), Associate Professor of Biomedical Sciences (SUNY) and Professor of Chemistry (Purdue). Since 2000 he has been Professor of Biochemistry in the Department of Molecular & Structural Biochemistry at North Carolina State University. Dr. Cavanagh has served on numerous NIH and NSF grant review panels and is currently a permanent member of the MSFB Study Section at NIH . He has authored over 100 peer-reviewed research publications and has been awarded the Foulerton Gift & Binmore Kenner Fellowship of the Royal Society (1990), the Fullsome Award (1996), the NC State University Alumni Associations Outstanding Research Award (2005) and Entrepreneur of the Year- NC State University (2012). He runs the Jimmy V-NCSU Cancer Therapeutics Training Program, was Assistant Vice Chancellor for Research at NC State from 2012-2014 and is the co-founder and Chief Scientific Officer of Agile Sciences Inc., a Raleigh based biotechnology company focusing on antibiotic resistance.
Affiliations and Expertise
North Carolina State University, Department of Molecular & Structural Biology, Raleigh, NC, USA
Nicholas Skelton
Affiliations and Expertise
Genentech, Inc., San Francisco, CA, USA
Wayne Fairbrother
Affiliations and Expertise
Genentech, Inc., San Francisco, CA, USA
Mark Rance
Affiliations and Expertise
University of Cincinnati, Cincinnati, OH, USA
Arthur Palmer, III
Affiliations and Expertise
Columbia University, New York, NY, USA