Protein Expression in Animal Cells - 1st Edition - ISBN: 9780125641449, 9780080859415

Protein Expression in Animal Cells, Volume 43

1st Edition

Serial Editors: Leslie Wilson Paul Matsudaira
Serial Volume Editors: Michael Roth
eBook ISBN: 9780080859415
Imprint: Academic Press
Published Date: 8th September 1994
Page Count: 379
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Table of Contents

The Use of Naturally Occurring and Recombinant Viruses:

R.W. Compans and P.C. Roberts, Viruses as Model Systems in Cell Biology.

V.M. Olkkonen, P. Liljeström, P. Dupree, H. Garoff, and K. Simons, Expression of Exogenous Proteins in Mammalian Cells with the Simliki Forest Virus Vector.

R.C. Piper, J.W. Slot, G. Li, P.D. Stahl, and D.E. James, Recombinant Sindbis Virus as an Expression System for Cell Biology.

G. Odorizzi and I.S. Trowbridge, Recombinant Rous Sarcoma Virus Vectors for Avian Cells.

J.-K. Yee, T. Friedmann, and J.C. Burns, Generation of High-Titer Pseudotyped Retroviral Vectors with Very Broad Host Range.

H.Y. Naim and M.G. Roth, SV40 Virus Expression Vectors.

O.A. Weisz and C.E. Machamer, Use of Recombinant Vaccinia Virus Vectors for Cell Biology.

T.C. Becker, R.J. Noel, W.S. Coats, A.M. Gómez-Foix, T. Alam, R.D. Gerard, and C.B. Newgard, Use of Recombinant Adenovirus for Metabolic Engineering of Mammalian Cells.

D.Y. Ho, Amplicon-Based Herpes Simplex Virus Vectors.

P.A. Johnson and T. Friedmann, Replication-Defective Recombinant Herpes Simplex Virus Vectors.

The Use of Expression Plasmids in Continuous Cell Lines:

C.B. Brewer, Cytomegalovirus Plasmid Vectors for Permanent Lines of Polarized Epithelial Cells.

R.P. Hirt, N. Fasel, and J.-P. Kraehenbuhl, Inducible Protein Expression Using a Glucocorticoid-Sensitive Vector.

R.A. Chavez, Y.-T. Chen, W.K. Schmidt, L. Carnell, and H.-P. Moore, Expression of Exogenous Proteins in Cells with Regulated Secretory Pathways.

D.G. Cook, V.M.-Y. Lee, and R.W. Doms, Expression of Foreign Proteins in a Human Neuronal System.

T.E. Willnow and J. Herz, Homologous Recombination for Gene Replacement in Mouse Cell Lines.

T. Mattioni, J.-F. Louvion, and D. Picard, Regulation of Protein Activities by Fusion to Steroid Binding Domains.

S.A. Johnston and D.-C. Tang, Gene Gun Transfection of Animal Cells and Genetic Immunization. Index. Volumes in Series.


Description

Critically acclaimed for more than 25 years, the Methods in Cell Biology series provides an indispensable tool for the researcher. Each volume is carefully edited by experts to contain state-of-the-art reviews and step-by-step protocols. Techniques are described completely so that methods are made accessible to users.

Key Features

  • Describes both well-established and novel recombinant vector systems for expression of proteins
  • Presents methods for efficient delivery of recombinant genes into differentiated cells, tissues, and whole animals
  • Covers high-level and inducible systems, plus assays for protein expression
  • Provides beginning and advanced investigators and students with the information they need to choose the optimal viral or plasmid system for their protein
  • Practical, benchtop-style presentation works in lab and in the classroom

Readership

Researchers, students, and technicians in cell and developmental biology, biotechnology, and biochemistry, genetics and molecular biology, virology, neurobiology, pharmacology, and most other areas of biomedical research.


Details

No. of pages:
379
Language:
English
Copyright:
© Academic Press 1994
Published:
Imprint:
Academic Press
eBook ISBN:
9780080859415

About the Serial Editors

Leslie Wilson Serial Editor

Affiliations and Expertise

University of California, Santa Barbara, U.S.A.

Paul Matsudaira Serial Editor

Affiliations and Expertise

Whitehead Institute for Biomedical Research and Massachusetts Institute of Technology, Cambridge, U.S.A.

About the Serial Volume Editors

Michael Roth Serial Volume Editor

Affiliations and Expertise

Southwestern Medical Center, University of Texas, Dallas, U.S.A.