Methods widely used in the study of DNA binding proteins are presented in this volume. These include purification and protein characterization, assays of protein-DNA binding and protein-induced bending, and biochemical and genetic methods for probing the structure, energy, and specificity of protein-DNA interactions.
Biochemists, geneticists, molecular biologists, cell biologists, microbiologists, genetic engineers, analytical chemists, biophysicists, and industrial researchers working on protein products.
Purification and Characterization:
R.R. Burgess, Use of Polyethyleneimine in Purification of DNA Binding Proteins.
J.T. Kadonaga, Purification of Sequence-Specific Binding Proteins by DNA Affinity Chromatography.
T. Formosa, J. Barry, B.M. Alberts, and J. Greenblatt, Using Protein Affinity Chromatography to Probe Structure of Protein Machines.
D.L. Merkle and J.M. Berg, Metal Requirements for Nucleic Acid Binding Proteins.
K. Tovar and W. Hillen, Large-Scale Preparation of DNA Fragments for Physical Studies of Protein Binding.
G. P~aaarraga and R.E. Klevit, Multidimensional Nuclear Magnetic Resonance Spectroscopy DNA-Binding Proteins.
A. Joachimiak and P.B. Sigler, Crystallization of Protein-DNA Complexes.
DNA Binding and Bending:
J. Carey, Gel Retardation.
D.M. Crothers, M.R. Gartenberg, and T.E. Shrader, DNA Bending in Protein-DNA Complexes.
S. Sasse-Dwight and J.D. Gralla, Footprinting Protein-DNA Complexes in Vivo.
M. Dodson and H. Echols, Electron Microscopy of Protein-DNA Complexes.
W.A. Lim, R.T. Sauer, and A.D. Lander, Analysis of DNA-Protein Interactions by Affinity Coelectrophoresis.
J.W. Hockensmith, W.L. Kubasek, W.R. Vorachek, E.M. Evertsz, and P.H. von Hippel, Laser Cross-Linking Protein-Nucleic Acid Complexes.
M. Buckle, A. Fritsch, P. Roux, J. Geiselmann, and H. Buc, Kinetic Studies on Promoter-RNA Polymerase Complexes.
T.M. Lohman and W. Bujalowski, Thermodynamic Methods for Model-Independent Determination of Equilibrium Binding Isotherms for Protein-DNA Interactions: Spectroscopic Approaches to Monitor Binding.
M.T. Record, Jr., J.-H. Ha, and M.A. Fishe
- No. of pages:
- © Academic Press 1991
- 11th December 1991
- Academic Press
- eBook ISBN:
- Hardcover ISBN:
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