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Protein Biochemistry and Proteomics - 1st Edition - ISBN: 9780120885459, 9780080458434

Protein Biochemistry and Proteomics

1st Edition

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Author: Hubert Rehm
Paperback ISBN: 9780120885459
eBook ISBN: 9780080458434
Imprint: Academic Press
Published Date: 21st February 2006
Page Count: 256
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Table of Contents

The Concept of the Experimenter Abbreviations

  1. The Daily Bread
  2. 1 Making Buffers
  3. 2 Protein Determination
  4. 3 Gels
  5. 4 Staining Gels
  6. 5 Precipitate and Concentrating
  7. 6 Blotting
  8. 7 Autoradiography of Gels and Blots
  9. Ligand Binding
  10. 1 Radioactive Ligand Marking
  11. 2 Binding
  12. 3 Analysis of Binding Data
  13. 4 Crosslinking of Ligands
  14. 5 Purposes
  15. Solubilization of Membrane Proteins
  16. 1 Detergents
  17. 2 Solubilization
  18. Protein Detection via Functional Measurements
  19. 1 Translocators
  20. 2 Reconstitution
  21. 3 Flux Assay
  22. 4 Constructive Thoughts
  23. Cleaning and Purifying
  24. 1 Pure Fun
  25. 2 Conventional Purification Methods
  26. 3 Affinity Chromatography
  27. 4 The Purity Test
  28. 5 Profiting
  29. Antibodies
  30. 1 Production of Polyclonal Antibodies
  31. 2 Immunoprecipitation
  32. 3 Immunoaffinity Chromatography
  33. 4 Antibodies Against Unpurified Proteins
  34. 5 Immunological detection techniques
  35. Proteomics
  36. 1 Introduction
  37. 2 Sample taking
  38. 3 2D gel electrophoresis
  39. 4 Mass Spectroscopy of Peptides and Proteins
  40. 5 Protein Chips
  41. 6 Microsequencing
  42. 7 Strategy
  43. Subunits
  44. 1 Number & Stoichiometry of Subunits
  45. 2 What Holds Our World Together
  46. Glycoproteins
  47. 1 How, Where and for What Purpose are Proteins Glycosylated?
  48. 2 Detecting Glycoproteins in Gels
  49. 3 Detection of Glycoproteins on Blots
  50. 4 Deglycosylation
  51. 5 The Sugar Chains
  52. Treasure Island
  53. 1 Of the Paper
  54. 2 Of Writing a Paper
  55. Desert Planet
  56. Jaws
  57. 1 Suppliers
  58. 2 Suppliers by Product
    Last Things
    Index

Description

Hubert Rehm's Protein Biochemistry and Proteomics is more than a laboratory manual; it is a strategic guide that provides the reader with tips and tricks for more successful lab experiments. Using a conversational yet professional tone, Rehm provides an overview of a variety of methods in protein biochemistry/proteomics. He provides short and precise summaries of routine procedures as well as listings of the advantages and disadvantages of alternative methods. Readers will immediately sense that the author if very familiar with the challenges, and frustration of the daily lab routine. Never before has such an honest, tactical guide been available for those conducting lab experiments within the field of biochemistry.

Key Features

  • Shows how to avoid experimental dead ends and helps users develop an instinct for the right experiment at the right time
  • Contains short and precise summaries of routine procedures (e.g. column chromatography, gel electrophoresis), and lists the advantages and disadvantages of alternative methods
  • Includes over 100 detailed figures and tables
  • Contains a chapter on proteomics

Readership

Laboratory practitioners such as students, instructors, and professionals, conducting experiments within the field of protein biochemistry.


Details

No. of pages:
256
Language:
English
Copyright:
© Academic Press 2006
Published:
21st February 2006
Imprint:
Academic Press
Paperback ISBN:
9780120885459
eBook ISBN:
9780080458434

Reviews

“It will provide you with the necessary tools for success…if you are a protein biochemist, what are you waiting for? Grab the Experimenter and get experimenting!” --Weanée Kimblewood for LAB TIMES (2006)

Ratings and Reviews


About the Author

Hubert Rehm