Probes and Membrane Function

1st Edition - January 1, 1971
Editors: Britton Chance, Chuan-pu Lee, J. Kent Blasie
Language: English
eBook ISBN:
9 7 8 - 1 - 4 8 3 2 - 7 1 4 8 - 4

Probes of Structure and Function of Macromolecules and Membranes, Volume I: Probes and Membrane Function discusses the developments in techniques for the measurement of rapid… Read more

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Probes of Structure and Function of Macromolecules and Membranes, Volume I: Probes and Membrane Function discusses the developments in techniques for the measurement of rapid reactions and detection of enzymatic reactions in simple and complex systems. This book describes the application of the incisive techniques employed for the study of activity-related structural changes in enzymes to functionally related structural changes in membranes. The structural changes initiated in response to energy coupling and ion movements in mitochondria and axons are also elaborated. This publication covers the basic mechanisms by which structural changes can participate in energy coupling at the redox level of the hemoproteins that leads to the evaluation of the function of ubiquinone and cytochrome b in energy coupling. The function of factors that couple electron transport and energy conservation is likewise considered. This volume is a good reference for students and researchers conducting work on the structure and function of macromolecules and membranes.

Contributors

Preface

Contents of Volume II

Introductory Remarks

General Introduction to Responsive Probes

Part 1. Interpretation of Data from Probe Studies

Introduction to Fluorescence Probes

The Effects of Chemical Environment on Fluorescence Probes

Discussion

The Use of Specific Chromophoric Inhibitors in Investigations of Biological Reactions in Solution, in Crystals, and in Membranes

Structural Sensitive Aspects of the Electronic Spectrum

Discussion

Introduction to Magnetic Resonance Techniques

The Nuclear Magnetic Resonance Spectroscopy of Proteins

Paramagnetic Probes in Enzyme Systems

Enzyme-Ligand Interactions as Detected by Nuclear Relaxation Rates of Substrates and Inhibitors

Electron-Nuclear Double Resonance (ENDOR) from Randomly Oriented Biomolecules

Electron Paramagnetic Resonance of Single Crystals as Environmental Probes

Discussion

Optical Activity and Conformation of Proteins

Near Infrared Circular Dichroism: d → d Transitions in Hemoproteins

Discussion

Introduction to X-Ray Diffraction and Electron Microscopy Studies

Protein Conformational Changes Studied by Diffraction Techniques

Low-Angle X-Ray Diffraction of Biological Membranes

Probing Membrane Structure with the Electron Microscope

Characterization of a Hydrocarbon Soluble Protein/Lipid Complex

Discussion

Part 2. Structural Control of Reactivity of Membranes

State Changes of the Mitochondrial Membrane as Detected by the Fluorescence Probe 1-Anilino-8-naphthalene Sulfonic Acid

Probes of Macromolecular and Molecular Structure in the Membranes of Mitochondria and Submitochondrial Vesiqles

Fluorochrome Labeling of Chromatophores of Photosynthetic Bacteria with Auramine O

Fluorescence Changes in Dye-Treated Nerve following Electric Stimulation

Anilino-1, 8-naphthalene Sulfonate and Brom thymol Blue Responses to Membrane Energization

Kinetic Analysis of MNS Interaction with Submitochondrial Particles

Discussion

Spin Labeling of Submitochondrial Membrane

Discussion

Response of ANS to Ca++ Binding in Mitochondrial Membrane as Affected by Local Anesthetics

K+ Gradients in Submitochondrial Particles

K+ Transport in Submitochondrial Particles

Glutaraldehyde as a Probe of Metabolism-Linked Changes in the Mitochondrial Proteins

Calcium Transport in Sonicated Submitochondrial Particles

General Discussion: Responses of Probes to Ionic Gradients

Use of 8-Anilino-1-naphthalene Sulfonate as Conformational Probe on Biological Membranes

ANS Fluorescence as an Indicator of Ionic Interaction with Membranes

Interaction of Fluorescent Probes with Hemoglobin Free Erythrocyte Membranes

The Design of Fluorescent Probes for Membranes

Conformational Activity vs. Ion-Induced Osmotic Perturbation in the Transformation of Mitochondrial Ultrastructure

Is There a Mechanochemical Coupling in Mitochondrial Oxidative-Phosphorylation?

X-Ray Diffraction Analysis of Myelin

General Discussion: The Role of Structure in the Response and Location of Probes in Natural and Artificial Membrane Systems

The Problem of Cytochrome b

Approaches to the Relation of Structure and Function of the Inner Mitochondrial Membrane

Interactions of Ubiquinone and Cytochrome b in the Respiratory Chain

Plasticity in the Responses of Ubiquinone and Cytochrome b

Discussion

Localization of Succinate Dehydrogenase in the Mitochondrial Inner Membrane

The Two Sides of the Inner Mitochondrial Membrane

Biosynthetic Origin of Cytochrome Oxidase

Localization of Cytochrome c and Cytochrome Oxidase in the Mitochondrial Inner Membrane

On Branching among Mitochondrial Respiratory Chains

Where Is the Mitochondrial Binding Site for Cytochrome c?

The Presence of Denatured Mitochondrial Adenosine Triphosphatase in "Structural Protein" from Beef-Heart Mitochondria

Reconstitution of Highly Resolved Particles with Succinate Dehydrogenase and Coupling Factors

Contaminants in "Purified" Energy Transfer Factors of Oxidative Phosphorylation

Computer Analysis of Electron Transport Kinetics

Discussion: Ordering of Membrane Components: The Sidedness Problem

General Discussion on Part 2

Part 3. Instrumentation Developments

Computer Processing and Analysis of EPR Spectra

Rotating Drum Densitometry Techniques

The Electric Field Jump Relaxation Method

Chemical Applications of Light Scattering: Scattering of Coherent Light and Chemical Fluctuations

Tunable Lasers and Optical Sampling

Time-Sharing in Spectrophotometry and Fluorometry

Relaxation Methods

General Discussion on Part 3

Subject Index