Probes and Membrane Function

Probes and Membrane Function

1st Edition - January 1, 1971

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  • Editors: Britton Chance, Chuan-pu Lee, J. Kent Blasie
  • eBook ISBN: 9781483271484

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Probes of Structure and Function of Macromolecules and Membranes, Volume I: Probes and Membrane Function discusses the developments in techniques for the measurement of rapid reactions and detection of enzymatic reactions in simple and complex systems. This book describes the application of the incisive techniques employed for the study of activity-related structural changes in enzymes to functionally related structural changes in membranes. The structural changes initiated in response to energy coupling and ion movements in mitochondria and axons are also elaborated. This publication covers the basic mechanisms by which structural changes can participate in energy coupling at the redox level of the hemoproteins that leads to the evaluation of the function of ubiquinone and cytochrome b in energy coupling. The function of factors that couple electron transport and energy conservation is likewise considered. This volume is a good reference for students and researchers conducting work on the structure and function of macromolecules and membranes.

Table of Contents

  • Contributors


    Contents of Volume II

    Introductory Remarks

    General Introduction to Responsive Probes

    Part 1. Interpretation of Data from Probe Studies

    Introduction to Fluorescence Probes

    The Effects of Chemical Environment on Fluorescence Probes


    The Use of Specific Chromophoric Inhibitors in Investigations of Biological Reactions in Solution, in Crystals, and in Membranes

    Structural Sensitive Aspects of the Electronic Spectrum


    Introduction to Magnetic Resonance Techniques

    The Nuclear Magnetic Resonance Spectroscopy of Proteins

    Paramagnetic Probes in Enzyme Systems

    Enzyme-Ligand Interactions as Detected by Nuclear Relaxation Rates of Substrates and Inhibitors

    Electron-Nuclear Double Resonance (ENDOR) from Randomly Oriented Biomolecules

    Electron Paramagnetic Resonance of Single Crystals as Environmental Probes


    Optical Activity and Conformation of Proteins

    Near Infrared Circular Dichroism: d → d Transitions in Hemoproteins


    Introduction to X-Ray Diffraction and Electron Microscopy Studies

    Protein Conformational Changes Studied by Diffraction Techniques

    Low-Angle X-Ray Diffraction of Biological Membranes

    Probing Membrane Structure with the Electron Microscope

    Characterization of a Hydrocarbon Soluble Protein/Lipid Complex


    Part 2. Structural Control of Reactivity of Membranes

    State Changes of the Mitochondrial Membrane as Detected by the Fluorescence Probe 1-Anilino-8-naphthalene Sulfonic Acid

    Probes of Macromolecular and Molecular Structure in the Membranes of Mitochondria and Submitochondrial Vesiqles

    Fluorochrome Labeling of Chromatophores of Photosynthetic Bacteria with Auramine O

    Fluorescence Changes in Dye-Treated Nerve following Electric Stimulation

    Anilino-1, 8-naphthalene Sulfonate and Brom thymol Blue Responses to Membrane Energization

    Kinetic Analysis of MNS Interaction with Submitochondrial Particles


    Spin Labeling of Submitochondrial Membrane


    Response of ANS to Ca++ Binding in Mitochondrial Membrane as Affected by Local Anesthetics

    K+ Gradients in Submitochondrial Particles

    K+ Transport in Submitochondrial Particles

    Glutaraldehyde as a Probe of Metabolism-Linked Changes in the Mitochondrial Proteins

    Calcium Transport in Sonicated Submitochondrial Particles

    General Discussion: Responses of Probes to Ionic Gradients

    Use of 8-Anilino-1-naphthalene Sulfonate as Conformational Probe on Biological Membranes

    ANS Fluorescence as an Indicator of Ionic Interaction with Membranes

    Interaction of Fluorescent Probes with Hemoglobin Free Erythrocyte Membranes

    The Design of Fluorescent Probes for Membranes

    Conformational Activity vs. Ion-Induced Osmotic Perturbation in the Transformation of Mitochondrial Ultrastructure

    Is There a Mechanochemical Coupling in Mitochondrial Oxidative-Phosphorylation?

    X-Ray Diffraction Analysis of Myelin

    General Discussion: The Role of Structure in the Response and Location of Probes in Natural and Artificial Membrane Systems

    The Problem of Cytochrome b

    Approaches to the Relation of Structure and Function of the Inner Mitochondrial Membrane

    Interactions of Ubiquinone and Cytochrome b in the Respiratory Chain

    Plasticity in the Responses of Ubiquinone and Cytochrome b


    Localization of Succinate Dehydrogenase in the Mitochondrial Inner Membrane

    The Two Sides of the Inner Mitochondrial Membrane

    Biosynthetic Origin of Cytochrome Oxidase

    Localization of Cytochrome c and Cytochrome Oxidase in the Mitochondrial Inner Membrane

    On Branching among Mitochondrial Respiratory Chains

    Where Is the Mitochondrial Binding Site for Cytochrome c?

    The Presence of Denatured Mitochondrial Adenosine Triphosphatase in "Structural Protein" from Beef-Heart Mitochondria

    Reconstitution of Highly Resolved Particles with Succinate Dehydrogenase and Coupling Factors

    Contaminants in "Purified" Energy Transfer Factors of Oxidative Phosphorylation

    Computer Analysis of Electron Transport Kinetics

    Discussion: Ordering of Membrane Components: The Sidedness Problem

    General Discussion on Part 2

    Part 3. Instrumentation Developments

    Computer Processing and Analysis of EPR Spectra

    Rotating Drum Densitometry Techniques

    The Electric Field Jump Relaxation Method

    Chemical Applications of Light Scattering: Scattering of Coherent Light and Chemical Fluctuations

    Tunable Lasers and Optical Sampling

    Time-Sharing in Spectrophotometry and Fluorometry

    Relaxation Methods

    General Discussion on Part 3

    Subject Index

Product details

  • No. of pages: 582
  • Language: English
  • Copyright: © Academic Press 1971
  • Published: January 1, 1971
  • Imprint: Academic Press
  • eBook ISBN: 9781483271484

About the Editors

Britton Chance

Chuan-pu Lee

J. Kent Blasie

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