Principles of Molecular Virology

Principles of Molecular Virology, Fifth Edition, provides an introduction to modern virology. Viruses are submicroscopic, obligate intracellular parasites that are more diverse than all the bacterial, plant, and animal kingdoms combined. The book examines protein-protein, protein-nucleic acid, and protein-lipid interactions, which control the structure of virus particles; the ways in which viruses infect cells; how viruses replicate; and the effects of virus infection on host organisms.

The book begins with a history of virology, tracing the development of knowledge and research on virology. The remaining seven chapters deal with the function and formation of virus particles; the structure and complexity of virus genomes; virus replication; gene expression; virus infections; the effects of virus infection on the body and the body’s response to infection; and subviral agents, such as satellites, viroids, and prions. The text concludes with three appendices that feature a glossary and abbreviations; a classification of subcellular infectious agents; and an outline of the history of virology.

• Completely rewritten and updated
• Clear and easy to understand
• Examples covering important ideas in virology
• All new illustrations

Undergraduates and graduates in virology, molecular biology and microbiology; Medical students and infectious disease clinicians and immunologists; Those entering the virology research area, including plant virology and AIDS research

Preface to the Fifth Edition

Chapter 1 Introduction

What Are Viruses?

Are viruses alive?

The History of Virology

Living Host Systems

Cell Culture Methods

Serological/Immunological Methods

Ultrastructural Studies

Molecular Biology

Summary

Chapter 2 Particles

The Function and Formation of Virus Particles

Capsid symmetry and virus architecture

Helical capsids

Icosahedral (isometric) capsids

Enveloped viruses

Complex virus structures

Protein-nucleic acid interactions and genome packaging

Virus receptors: recognition and binding

Other interactions of the virus capsid with the host cell

Summary

Chapter 3 Genomes

The Structure and Complexity of Virus Genomes

Molecular genetics

Virus genetics

Virus mutants

Genetic interactions between viruses

Nongenetic interactions between viruses

Small DNA genomes

Large DNA genomes

Positive-strand RNA viruses

Negative-strand RNA viruses

Segmented and multipartite virus genomes

Reverse transcription and transposition

Evolution and epidemiology

Summary

Chapter 4 Replication

Overview of Virus Replication

Investigation of Virus Replication

The Replication Cycle

Attachment

Penetration

Uncoating

Genome replication and gene expression

Assembly

Maturation

Release

Summary

Chapter 5 Expression

Expression of Genetic Information

Control of Prokaryote Gene Expression

Control of Expression in Bacteriophage ?

Control of Eukaryote Gene Expression

Genome Coding Strategies

Class I: Double-stranded DNA

Class II: Single-stranded DNA

Class III: Double-stranded RNA

Class IV: Single-stranded (+)sense RNA

Class V: Single-stranded (-)sense RNA

Class VI: Single-stranded (+)sense RNA with DNA intermediate

Class VII: Double-stranded DNA with RNA intermediate

Transcriptional Control of Expression

Posttranscriptional Control of Expression

Summary

Chapter 6 Infection

Virus Infections of Plants

Immune Responses to Virus Infections in Animals

Viruses and Apoptosis

Interferons

Evasion of Immune Responses by Viruses

Inhibition of MHC-I-restricted antigen presentation

Inhibition of MHC-II-restricted antigen presentation

Inhibition of natural killer cell lysis

Interference with apoptosis

Inhibition of cytokine action

Evasion of humoral immunity

Evasion of the complement cascade

Virus-Host Interactions

The Course of Virus Infections

Abortive infection

Acute infection

Chronic infection

Persistent infection

Latent infection

Prevention and Therapy of Virus Infection

RNA Interference (RNAi)

Virus Vectors and Gene Therapy

Chemotherapy of Virus Infections

Summary

Chapter 7 Pathogenesis

Mechanisms of Cellular Injury

Viruses and Immunodeficiency

Virus-Related Diseases

Bacteriophages and Human Disease

Cell Transformation by Viruses

Cell Transformation by Retroviruses

Cell Transformation by DNA Viruses

Viruses and Cancer

New and Emergent Viruses

Zoonoses

Bioterrorism

Summary

Chapter 8 Subviral Agents: Genomes without Viruses, Viruses without Genomes

Satellites and Viroids

Prions

Pathology of prion diseases

TSE in Animals

Scrapie

Transmissible mink encephalopathy (TME)

Feline spongiform encephalopathy (FSE)

Chronic wasting disease (CWD)

Bovine spongiform encephalopathy (BSE)

Human TSES

Molecular Biology of Prions

Summary

Appendix 1: Glossary and Abbreviations

Appendix 2: Classification of Subcellular Infectious Agents

Appendix 3: The History of Virology

Index