Principles and Practice of Modern Chromatographic Methods

Principles and Practice of Modern Chromatographic Methods

2nd Edition - December 3, 2021

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  • Authors: Kevin Robards, Danielle Ryan
  • eBook ISBN: 9780128220979
  • Paperback ISBN: 9780128220962

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Description

Principles and Practice of Modern Chromatographic Methods, Second Edition takes a comprehensive, unified approach in its presentation of chromatographic techniques. Like the first edition, the book provides a scientifically rigid, but easy-to-follow presentation of chromatography concepts that begins with the purpose and intent of chromatographic theory - the “what and why” that are left out of other books attempting to cover these principles. This fully revised second edition brings the content up-to-date, covering recent developments in several new sections and an additional chapter on composite methods. New topics include sample profiling, sample preparation, sustainable green chemistry, 2D chromatography, miniaturization/nano-LC, HILIC, and more.

Key Features

  • Contains thorough chapters that begin with an updated schematic overview and a visual representation of the content
  • Avoids the obfuscation of different terminologies and classification systems that are prevalent in the area, such as the relationship between liquid chromatography and column chromatography
  • Provides integrated and comprehensive topic coverage based on chromatographic bibliometrics and survey reports on the relative usage of chromatographic techniques

Readership

Practitioners and researchers studying chromatography or using it to solve problems in clinical chemistry, geology, cosmetics, food science, petroleum, etc. Students (postgraduate, graduate and undergraduate) and professors taking or teaching a course in chromatography

Table of Contents

  • Cover image
  • Title page
  • Table of Contents
  • Copyright
  • Preface
  • Preface to first edition
  • Acknowledgements
  • Caution
  • Chapter 1: Introduction and overview
  • Abstract
  • 1.1: Introduction
  • 1.2: Coverage
  • 1.3: Chromatographic separation simply explained
  • 1.4: Classification of chromatography
  • 1.5: Chromatography: Publications and equipment sales
  • 1.6: Applications of chromatography
  • 1.7: Comparison of chromatographic techniques
  • 1.8: Historical aspects
  • 1.9: Obtaining assistance
  • References
  • Chapter 2: Theoretical considerations
  • Abstract
  • 2.1: Introduction
  • 2.2: Theory of chromatography
  • 2.3: Retention, dispersion, and distortion
  • 2.4: Retention: Thermodynamics and molecular interactions
  • 2.5: Retention
  • 2.6: Measures of chromatographic retention
  • 2.7: Dispersion
  • 2.8: Is it resolution or separation?
  • 2.9: Extra-column dispersion
  • 2.10: Summary: Retention and dispersion
  • 2.11: Column testing
  • References
  • Chapter 3: Planar chromatography
  • Abstract
  • 3.1: Introduction
  • 3.2: Why thin layer chromatography?
  • 3.3: Theoretical considerations
  • 3.4: Sample application in TLC
  • 3.5: Plate development in TLC
  • 3.6: Innovation in TLC
  • 3.7: Thin layer plates
  • 3.8: Stationary phases
  • 3.9: Mobile phases
  • 3.10: Detection: Qualitative analysis
  • 3.11: Quantitative analysis
  • 3.12: Separations
  • References
  • Chapter 4: Gas chromatography
  • Abstract
  • 4.1: Introduction
  • 4.2: Mobile phases and delivery systems
  • 4.3: Sample introduction in GC
  • 4.4: Column packings and hardware
  • 4.5: Detectors
  • 4.6: Column temperature and temperature programming
  • 4.7: Derivatization
  • 4.8: Innovation
  • 4.9: Separations
  • References
  • Chapter 5: High performance liquid chromatography: Instrumentation and techniques
  • Abstract
  • 5.1: Introduction
  • 5.2: From HPLC to UHPLC and beyond
  • 5.3: Solvent delivery systems
  • 5.4: Sample introduction in HPLC
  • 5.5: Column packings and hardware
  • 5.6: Detectors
  • 5.7: Post-column derivatization
  • 5.8: Gradient elution
  • 5.9: Multidimensional liquid chromatography
  • References
  • Chapter 6: High performance liquid chromatography: Separations
  • Abstract
  • 6.1: Introduction
  • 6.2: Separation of neutral compounds
  • 6.3: Techniques for ionic and ionizable species
  • 6.4: Specialty separation modes
  • 6.5: Choosing a chromatographic method
  • References
  • Chapter 7: Supercritical fluid chromatography
  • Abstract
  • 7.1: Introduction
  • 7.2: Mobile phases and delivery systems
  • 7.3: Sample introduction in SFC
  • 7.4: Column packings and hardware
  • 7.5: Detectors
  • 7.6: Programming techniques
  • 7.7: Separations
  • References
  • Chapter 8: Coupled systems
  • Abstract
  • 8.1: Introduction
  • 8.2: Multidimensional chromatography
  • 8.3: Hyphenated techniques
  • References
  • Chapter 9: Analyses
  • Abstract
  • 9.1: Introduction
  • 9.2: Detectors for chromatography
  • 9.3: Data handling
  • 9.4: Qualitative analysis
  • 9.5: Quantitative analysis
  • 9.6: Metabolomics and chromatography
  • References
  • Chapter 10: Sample handling in chromatography
  • Abstract
  • 10.1: Introduction
  • 10.2: Why bother with sample handling?
  • 10.3: Sample collection procedures
  • 10.4: Sustainable green chemistry
  • 10.5: Sample preparation
  • 10.6: Pre-concentration techniques
  • References
  • Chapter 11: Preparative chromatography
  • Abstract
  • 11.1: Introduction
  • 11.2: Thin layer chromatography
  • 11.3: Gas chromatography
  • 11.4: Supercritical fluid chromatography
  • 11.5: High-performance liquid chromatography
  • References
  • Index

Product details

  • No. of pages: 530
  • Language: English
  • Copyright: © Academic Press 2021
  • Published: December 3, 2021
  • Imprint: Academic Press
  • eBook ISBN: 9780128220979
  • Paperback ISBN: 9780128220962

About the Authors

Kevin Robards

Kevin Robards majored in analytical chemistry and biochemistry. He worked in industry with edible oils and completed an Honours thesis on synthetic antioxidants. After some years investigating complexation chemistry and chromatography and a brief period examining the agricultural applications of rare earths, he returned to his passion, antioxidants, only now looking at naturally occurring members such as biophenols. The last years of his employment were divided between biophenolic research and studying academic and corporate governance. After five decades of research and teaching at all levels (undergraduate through post-doctoral) in analytical chemistry with a specific emphasis on chromatography, Professor Robards has now retired and was granted Emeritus status in recognition of his contribution to the academy.

Affiliations and Expertise

Emeritus Professor, Charles Sturt University, Wagga Wagga, Australia

Danielle Ryan

Danielle Ryan majored in analytical chemistry. After her PhD at Charles Sturt University (CSU) she completed post-doctoral research in comprehensive two-dimensional gas chromatography at RMIT University before taking up an academic position at CSU. Danielle’s research is focused on the application of analytical chemistry and separation science to complex samples to understand natural product quality and identify biomarkers associated with human diseases.

Affiliations and Expertise

Senior Lecturer in Chemistry, Charles Sturt University, Wagga Wagga, Australia

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