Practical Biochemistry for Colleges

This book presents a selection of tried and trusted laboratory experiments in the field of biochemistry. The experiments are described in detail and can be used directly or in a modified form. They are grouped according to a broad range of biochemical disciplines which allows those responsible for arranging practical classes to select experiments to complement any given biochemistry course. Suggestions are made for further work in more advanced classes. As well as the practical method the experiments are accompanied by background information, discussion of results, references for further study and illustrations.

For biochemistry teachers and technicians in universities, technical colleges and high schools.

Section Headings and Selected Papers: Enzymes and Enzymology. Some properties of an enzyme: a demonstration experiment with alkaline phosphatase, D F Evered. A simple laboratory experiment to demonstrate transamination, S Dewhurst & I Smallman. Biochemical Techniques. Experiments illustrating gel filtration, J M Wallach. Gel filtration of haemoglobin, H B F Dixon. Metabolism. Glycolysis and gluconeogenesis, R W Hanson. Bottled glycolysis, H A Akers & J M Long. Molecular Biology. Analysis of chromatin structure and composition, J E Perez-Ortin et al. Isolation of biologically active mRNA, S Spindler et al. Model Building. Molecular models: paper formulae and pop-up models, B S Brown & E M Evans. The use of paper molecular models as an aid in lectures on oligosaccharide and polysaccharide structures, M Alemany. Clinical Biochemistry. The sucrose content of breakfast cereals, D P Robson & T P Chirpich. The determination of sialic acid - an experiment that demonstrates many important aspects of spectrophotometric assays, R O'Kennedy. Index.