- Print ISBN 9780444563514
- Electronic ISBN 9780444563590
The book reviews the use of spectroscopic and related methods to investigate the complex structures and mechanisms of biological inorganic systems that contain metals. Each chapter presents an overview of the technique including relevant theory, clearly explains what it is and how it works and then presents how the technique is actually used to evaluate biological structures. Practical examples and problems are included to illustrate each technique and to aid understanding. Designed for students and researchers who want to learn both the basics, and more advanced aspects of bioinorganic chemistry.
Chapter 1. An Overview of the Roles of Metals in Biological Systems
Introduction: Which Metals Ions and Why?
Some Physicochemical Considerations on Alkali Metals
Na+ and K+ – Functional Ionic Gradients
Mg2+ – Phosphate Metabolism
Ca2+ and Cell Signalling
Zinc – Lewis Acid and Gene Regulator
Iron and Copper – Dealing with Oxygen
Ni and Co – Evolutionary Relics
Mn – Water Splitting and Oxygen Generation
Mo and V – Nitrogen Fixation
Chapter 2. Introduction to Ligand Field Theory
Crystal Field and Ligand Field Theory
MO Theory of Transition Metal Complexes
Chapter 3. EPR Spectroscopy
Why EPR Spectroscopy?
What Is EPR Spectroscopy?
A Comparison of EPR Versus NMR
What (Bio)molecules Give EPR?
Basic Theory and Simulation of EPR
Chapter 4. Introduction to Biomolecular NMR and Metals
Properties of the Matter Relevant to NMR
Energy of NMR Transitions
Acting on Magnetisation
An NMR Experiment
The Chemical Shift
Coupling: The Interaction Between Magnetic Nuclei
The Nuclear Overhauser Effect
Metals in Biomolecular NMR Spectra
An NMR Spectrometer and How the Macroscopic Magnetisation and Relaxation are Measured
Care in Obtaining NMR Spectra of Paramagnetic Samples
Useful Physical Const
"Editors Crichton and Louro offer this new bio-inorganic chemistry text, focusing largely on analytical techniques…Strategic technique selection is emphasized throughout, for example, matching precision and expense of analysis to the quality of the sample."--Reference & Research Book News, October 2013