Description

The book reviews the use of spectroscopic and related methods to investigate the complex structures and mechanisms of biological inorganic systems that contain metals. Each chapter presents an overview of the technique including relevant theory, clearly explains what it is and how it works and then presents how the technique is actually used to evaluate biological structures. Practical examples and problems are included to illustrate each technique and to aid understanding. Designed for students and researchers who want to learn both the basics, and more advanced aspects of bioinorganic chemistry.

Key Features

  • Many colour illustrations enable easier visualization of molecular mechanisms and structures
  • Worked examples and problems are included to illustrate and test the reader’s understanding of each technique
  • Written by a multi-author team who use and teach the most important techniques used today to analyse complex biological structures

Readership

Postgraduates or postdocs plus chemical and biological researchers investigating biological structures

Table of Contents

Preface

Chapter 1. An Overview of the Roles of Metals in Biological Systems

Introduction: Which Metals Ions and Why?

Some Physicochemical Considerations on Alkali Metals

Na+ and K+ – Functional Ionic Gradients

Mg2+ – Phosphate Metabolism

Ca2+ and Cell Signalling

Zinc – Lewis Acid and Gene Regulator

Iron and Copper – Dealing with Oxygen

Ni and Co – Evolutionary Relics

Mn – Water Splitting and Oxygen Generation

Mo and V – Nitrogen Fixation

References

Chapter 2. Introduction to Ligand Field Theory

Introduction

Crystal Field and Ligand Field Theory

MO Theory of Transition Metal Complexes

Concluding Remarks

Further Reading

Chapter 3. EPR Spectroscopy

Why EPR Spectroscopy?

What Is EPR Spectroscopy?

Anisotropy

A Comparison of EPR Versus NMR

EPR Spectrometer

What (Bio)molecules Give EPR?

Basic Theory and Simulation of EPR

Saturation

Concentration Determination

Hyperfine Interactions

High-Spin Systems

Applications Overview

References

Chapter 4. Introduction to Biomolecular NMR and Metals

Introduction

Properties of the Matter Relevant to NMR

Energy of NMR Transitions

Macroscopic Magnetisation

Acting on Magnetisation

Relaxation

An NMR Experiment

The Chemical Shift

Coupling: The Interaction Between Magnetic Nuclei

Chemical Exchange

The Nuclear Overhauser Effect

Multidimensional NMR

Metals in Biomolecular NMR Spectra

Relaxation

An NMR Spectrometer and How the Macroscopic Magnetisation and Relaxation are Measured

Care in Obtaining NMR Spectra of Paramagnetic Samples

Conclusions

References

Useful Physical Const

Details

No. of pages:
336
Language:
English
Copyright:
© 2013
Published:
Imprint:
Elsevier
Print ISBN:
9780444563514
Electronic ISBN:
9780444563590

About the authors

Robert Crichton

Unité de Biochimie, Université Catholique de Louvain, Louvain-la-Neuve, Belgium Prof. Crichton, originally from the UK, is well known in the inorganic biology community and is invited to speak at many international conferences. He has published 3 books in this area (others on biochemistry of iron and metal-based neruodegeneration) and has taught over 750 doctoral and post-doctoral students. The book contains an essential distillation of the knowledge and material he had used to teach and lecture over many years.

Affiliations and Expertise

Unité de Biochimie, Université Catholique de Louvain, Louvain-la-Neuve, Belgium

Ricardo Louro

Affiliations and Expertise

Inorganic Biochemistry and NMR Laboratory ITQB-UNL Av da Republica (EAN), Portugal

Reviews

"Editors Crichton and Louro offer this new bio-inorganic chemistry text, focusing largely on analytical techniques…Strategic technique selection is emphasized throughout, for example, matching precision and expense of analysis to the quality of the sample."--Reference & Research Book News, October 2013