Post-translational Modifications That Modulate Enzyme Activity - 1st Edition - ISBN: 9780128186695

Post-translational Modifications That Modulate Enzyme Activity, Volume 626

1st Edition

Serial Volume Editors: Benjamin Garcia
Hardcover ISBN: 9780128186695
Imprint: Academic Press
Published Date: 1st October 2019
Page Count: 412
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Table of Contents

1. Crosstalk between cellular metabolism and histone acetylation
Nathaniel Snyder
2. Isolation of protein complexes and modifications that regulate transcriptional machinery
Michael Washburn
3. High-throughput phosphoproteome mapping through multiplexed mass spectrometry
Wilhelm Haas
4. Differentiation of D and L epimerization in proteins
Ryan Julian
5. Biochemical analysis of protein arginylation
Anna Kashina
6. Site-specific Determination of lysine acetylation stoichiometries on the proteome-scale
Yue Chen
7. Genomic and biochemical analysis of RNA post-transcriptional modifications
Fange Liu
8. Isolation and characterization of glycosylated (neuro)peptides
Lingjun Li
9. Global detection of protein sialylation and phosphorylation by mass spectrometry
Martin R. Larsen
10. Split Protein trans-splicing for creation of site-specifically modified proteins in vivo
Yael David
11. Global and site-specific analysis of protein N-glycosylation with mass spectrometry (MS)-based proteomics methods
Ronghu Wu
12. Small molecule interrogation of the glycome
Mia Huang
13. Probing the structure and function of N-terminal acetyltransferases
Ronen Marmorstein
14. Proteomic Identification of the Substrates of Poly-ADP-Ribose Polymerases
Yonghao Yu
15. Analysis of the topology of ubiquitin chains
Catherine Fenselau
16. Intact protein analysis of troponin proteoforms
Ying Ge
17. Assays for tyrosine phosphorylation in human cells
Laurie Parker
18. Inhibiting lipid metabolism enzyme activity
Ku-Lung (Ken) Hsu
19. Identification and function of N-terminal glyoxylyl modification on proteins
Megan Matthews
20. Chromatin immunoprecipitation (ChIP)-Seq analysis of the genomic distribution of histone modifications
Michael-Christopher Keogh
21. Identification and characterization of novel protease substrates
Christopher Overall
22. Methods for the expression, purification, and crystallization of histone deacetylase 6-inhibitor complexes
David W. Christianson
23. Chemical tools to study redox modifications of cysteine thiols
Kate Carroll
24. Quantitative analysis of global protein methylation by mass spectrometry
Benjamin A. Garcia
25. Characterization of oxidative protein post-translational modifications
Rena AS Robinson
26. Expression of authentic post-translationally modified proteins in organisms with expanded genetic codes
Jesse Rinehart
27. Preparation of a New Construct of Histone Deacetylase 8 for the Crystallization of Enzyme-Inhibitor Complexes
David W. Christianson
28. Analysis of protein deacetylase activity during viral infection
Ileana M. Cristea


Description

Post-translational Modifications That Modulate Enzyme Activity, Volume 626 in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Updated chapters include Crosstalk between cellular metabolism and histone acetylation, Isolation of protein complexes and modifications that regulate transcriptional machinery, High-throughput phosphoproteome mapping through multiplexed mass spectrometry, Differentiation of D and L epimerization in proteins, Biochemical analysis of protein arginylation, Site-specific Determination of lysine acetylation stoichiometries on the proteome-scale, Genomic and biochemical analysis of RNA post-transcriptional modifications, Isolation and characterization of glycosylated (neuro)peptides, and more.

Key Features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods in Enzymology series
  • Includes the latest information on Post-translational Modifications that Modulate Enzyme Activity

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists


Details

No. of pages:
412
Language:
English
Copyright:
© Academic Press 2019
Published:
Imprint:
Academic Press
Hardcover ISBN:
9780128186695

Ratings and Reviews


About the Serial Volume Editors

Benjamin Garcia Serial Volume Editor

Benjamin A. Garcia, Ph.D., Presidential Professor, Professor of Biochemistry and Biophysics, Director of Quantitative Proteomics, Penn Epigenetics Institute, Department of Biochemistry and Biophysics, Smilow Center for Translational Research, University of Pennsylvania School of Medicine

Affiliations and Expertise

University of Pennsylvania, PA, USA