Post-translational Modifications That Modulate Enzyme Activity

Post-translational Modifications That Modulate Enzyme Activity

1st Edition - October 1, 2019

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  • Editor: Benjamin Garcia
  • eBook ISBN: 9780128186701
  • Hardcover ISBN: 9780128186695

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Post-translational Modifications That Modulate Enzyme Activity, Volume 626 in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Updated chapters include Crosstalk between cellular metabolism and histone acetylation, Isolation of protein complexes and modifications that regulate transcriptional machinery, High-throughput phosphoproteome mapping through multiplexed mass spectrometry, Differentiation of D and L epimerization in proteins, Biochemical analysis of protein arginylation, Site-specific Determination of lysine acetylation stoichiometries on the proteome-scale, Genomic and biochemical analysis of RNA post-transcriptional modifications, Isolation and characterization of glycosylated (neuro)peptides, and more.

Key Features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods in Enzymology series
  • Includes the latest information on Post-translational Modifications that Modulate Enzyme Activity


Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Table of Contents

  • 1. Crosstalk between cellular metabolism and histone acetylation
    Sophie Trefely, Mary T. Doan and Nathaniel W. Snyder
    2. Purification and enzymatic assay of class I histone deacetylase enzymes
    Mark K. Adams, Charles A.S. Banks, Sayem Miah, Maxime Killer and Michael P. Washburn
    3. Multiplexed quantitative phosphoproteomics of cell line and tissue samples
    Johannes Kreuzer, Amanda Edwards and Wilhelm Haas
    4. Differentiation of peptide isomers and epimers by radical-directed dissociation
    Tyler R. Lambeth and Ryan R. Julian
    5. Biochemical analysis of protein arginylation
    Junling Wang, John R. Yates and Anna Kashina
    6. Site-specific determination of lysine acetylation stoichiometries on the proteome-scale
    Yue Chen and Yunan Li
    7. RNA modifications and the link to human disease
    Amber Yanas and Kathy Fange Liu
    8. Isolation and characterization of glycosylated neuropeptides
    Yang Liu, Qinjingwen Cao and Lingjun Li
    9. Utilizing intein trans-splicing for in vivo generation of site-specifically modified proteins
    Igor Maksimovic, Devin Ray, Qingfei Zheng and Yael David
    10. Systematic and site-specific analysis of N-glycoproteins on the cell surface by integrating bioorthogonal chemistry and MS-based proteomics
    Fangxu Sun and Ronghu Wu
    11. Silencing glycosaminoglycan functions in mouse embryonic stem cells with small molecule antagonists
    Sourav Chatterjee, Tesia N. Stephenson, Austen L. Michalak, Kamil Godula and Mia L. Huang
    12. Biochemical and structural analysis of N-terminal acetyltransferases
    Leah Gottlieb and Ronen Marmorstein
    13. Site-specific analysis of the Asp- and Glu-ADP-ribosylated proteome by quantitative mass spectrometry
    Peng Li, Yuanli Zhen and Yonghao Yu
    14. Analysis of the topology of ubiquitin chains
    Lucia Geis-Asteggiante, Amanda E. Lee and Catherine Fenselau
    15. Analysis of cardiac troponin proteoforms by top-down mass spectrometry
    Timothy N. Tiambeng, Trisha Tucholski, Zhijie Wu, Yanlong Zhu, Stanford D. Mitchell, David S. Roberts, Yutong Jin and Ying Ge
    16. Assays for tyrosine phosphorylation in human cells
    Monica Kruk, Naomi Widstrom, Sampreeti Jena, Nicole L. Wolter, John F. Blankenhorn, Ibrahim Abdalla, Tzu-Yi Yang and Laurie L. Parker
    17. Metabolomics analysis of lipid metabolizing enzyme activity
    Timothy B. Ware, Myungsun Shin and Ku-Lung Hsu
    18. Simplified high yield TAILS terminomics using a new HPG-ALD 800K-2000 polymer with precipitation
    Nestor Solis, Anilkumar Parambath, Srinivas Abbina, Jayachandran Kizhakkedathu and Christopher M. Overall
    19. Methods for the expression, purification, and crystallization of histone deacetylase 6–inhibitor complexes
    Jeremy D. Osko and David W. Christianson
    20. Quantitative analysis of global protein lysine methylation by mass spectrometry
    Peder J. Lund, Stephanie M. Lehman and Benjamin A. Garcia
    21. The roles of S-nitrosylation and S-glutathionylation in Alzheimer’s disease
    Ryan R. Dyer, Katarena I. Ford and Renã A. S. Robinson
    22. Expression of authentic post-translationally modified proteins in organisms with expanded genetic codes
    Kyle Mohler and Jesse Rinehart
    23. Preparation of a new construct of human histone deacetylase 8 for the crystallization of enzyme-inhibitor complexes
    Nicholas J. Porter and David W. Christianson
    24. Methods for characterizing protein acetylation during viral infection
    Laura A. Murray, Ashton N. Combs, Pranav Rekapalli and Ileana M. Cristea

Product details

  • No. of pages: 648
  • Language: English
  • Copyright: © Academic Press 2019
  • Published: October 1, 2019
  • Imprint: Academic Press
  • eBook ISBN: 9780128186701
  • Hardcover ISBN: 9780128186695

About the Serial Volume Editor

Benjamin Garcia

Benjamin A. Garcia, Ph.D., Presidential Professor, Professor of Biochemistry and Biophysics, Director of Quantitative Proteomics, Penn Epigenetics Institute, Department of Biochemistry and Biophysics, Smilow Center for Translational Research, University of Pennsylvania School of Medicine

Affiliations and Expertise

University of Pennsylvania, PA, USA

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