Polyphenols in Plants
1st Edition
Isolation, Purification and Extract Preparation
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Description
Polyphenols in Plants assists plant scientists and dietary supplement producers in assessing polyphenol content and factors affecting their composition. It also aids in selecting sources and regulating environmental conditions affecting yield for more consistent and function dietary supplements.
Polyphenols play key roles in the growth, regulation and structure of plants and vary widely within different plants. Stress, growth conditions and plant species modify polyphenol structure and content. This book describes techniques to identify, isolate and characterize polyphenols, taking mammalian toxicology into account as well.
Key Features
- Defines conditions of growth affecting the polyphenol levels
- Describes assay and instrumentation techniques critical to identifying and defining polyphenols, critical to researchers and business development
- Documents how some polyphenols are dangerous to consume, important to dietary supplement industry, government regulators and lay public users
Table of Contents
Preface
Acknowledgement
Contributors
Part 1 Modification by Plant Growth and Environment
Chapter 1 Cultivar and Production Effects on Bioactive Polyphenols
1.1 Introduction
1.2 Effects of plant species, cultivar and breeding success
1.3 Light effects
1.4 Effects of growing temperature
1.5 Impact of water management
1.6 Effects of elevated carbon dioxide concentrations
1.7 Macro- and micronutrients and heavy metals induce changes in polyphenols
1.8 The application of electricity as a new approach in the horticultural sector
Chapter 2 Plant Polyphenol Profiles as a Tool for Traceability and Valuable Support to Biodiversity
2.1 Introduction
2.2 Traceability: definition, importance and state of the art in the area concerned
2.3 Traceability markers
2.4 Polyphenols as traceability markers
2.5 Examples of the use of polyphenols as markers
2.6 Concluding remarks
Section 1A Stress and Polyphenols in Plants
Chapter 3 Phenolic Compounds and Saponins in Plants Grown Under Different Irrigation Regimes
3.1 Introduction
3.2 Secondary metabolites: phenolic compounds and saponins
3.3 Factors that influence plants’ secondary metabolism
3.4 Influence of the irrigation regimes on secondary metabolites
3.5 Concluding remarks
Chapter 4 Lichen Phenolics: Environmental Effects
4.1 Introduction
4.2 Effects of environmental factors on the production of lichen phenolics
4.3 Role of photobionts in phenolics production
4.4 Conclusions
Section 1B Plant systems of polyphenol modification
Chapter 5 Modulation of Plant Endogenous Antioxidant Systems by Polyphenols
5.1 Introduction
5.2 Plant oxidative stress
5.3 Antioxidant defense system
5.4 Polyphenol properties
5.5 Concluding remarks
Chapter 6 Plant Polyphenols: Do They Control Freshwater Planktonic Nuisance Phototrophs?
6.1 Introduction
6.2 Role of ambient pH
6.3 Physiological state of the phototrophs
6.4 Action of allelochemicals other than polyphenols
6.5 Modes of action in Microcystis aeruginosa
6.6 Conclusion from an ecological perspective
Associated content
Part 2 Isolation and Analysis of Polyphenol Structure
Section 2A Analysis Techniques for Polyphenols
Chapter 7 Gas Chromatography–Mass Spectrometry Analysis of Polyphenols in Foods
7.1 Polyphenolic compounds
7.2 Polyphenolic compounds in foods
7.3 Methods for determining polyphenols
7.4 Determination of polyphenols by gas chromatography–mass spectrometry (GC-MS). Derivatization reactions
7.5 Sample preparation techniques
7.6 Miniaturized techniques for sample preparation in GC-MS
Chapter 8 Novel Techniques Towards the Identification of Different Classes of Polyphenols
8.1 Introduction
8.2 Sample treatment and extraction
8.3 High-performance liquid chromatography (HPLC)
8.4 Liquid chromatography coupled to mass spectrometry (LC-MS) and direct flow injection mass spectrometry
8.5 Nuclear magnetic resonance spectroscopy (NMR)
8.6 Liquid chromatography coupled to nuclear magnetic resonance spectroscopy (LC-NMR)
8.7 Other methods
Chapter 9 Characterization of Polyphenolic Profile of Citrus Fruit by HPLC/PDA/ESI/MS-MS
9.1 Introduction
9.2 Polyphenolic compounds in citrus cultivars
9.3 HPLC/PDA/ESI/MS-MS identification of citrus flavonoids
Section 2B Isolation and Extraction Techniques
Chapter 10 Non-Extractable Polyphenols in Plant Foods: Nature, Isolation, and Analysis
10.1 Introduction
10.2 Nature of NEPP
10.3 Isolation of NEPP
10.4 Analysis of NEPPS
10.5 NEPP content in plant foods
10.6 Potential applications of NEPP
10.7 Concluding remarks
Chapter 11 Resin Adsorption and Ion Exchange to Recover and Fractionate Polyphenols
11.1 Adsorption and ion exchange technology—from past to present and future applications
11.2 Adsorbent and ion exchange materials
11.3 Principles of adsorption and ion exchange
11.4 Application of adsorption and ion exchange technology
11.5 Application of adsorbent resins and ion exchangers for the recovery of bio- and techno-functional phenolic compounds from by-products of food processing
Chapter 12 Polyphenolic Compounds from Flowers of Hibiscus: Characterization and Bioactivity
Chapter 13 Hydrothermal Processing on Phenols and Polyphenols in Vegetables
Part 3 Polyphenols Identification and Occurrence
Chapter 14 Improved Characterization of Polyphenols Using Liquid Chromatography
14.1 Introduction
14.2 Sample preparation
14.3 High performance liquid chromatography (HPLC)
14.4 Ultra-high-performance liquid chromatography (UHPLC)
14.5 Conclusions
Chapter 15 Characterization and Quantification of Polyphenols in Fruits
15.1 Introduction
15.2 Sample preparation
15.3 Analytical methods
Chapter 16 Determination of Polyphenols, Flavonoids, and Antioxidant Capacity in Dry Seeds
16.1 Introduction
16.2 Extraction of polyphenols from seeds
16.3 Additional extraction techniques for seed polyphenols
16.4 Methods for quantification of total polyphenols, total flavonoids and antioxidant activity in seeds
Index
Details
- No. of pages:
- 360
- Language:
- English
- Copyright:
- © Academic Press 2014
- Published:
- 7th February 2014
- Imprint:
- Academic Press
- Hardcover ISBN:
- 9780123979346
- eBook ISBN:
- 9780123984913
About the Editor

Ronald Watson
Ronald Ross Watson, PhD, is Professor of Health Promotion Sciences at the University of Arizona, Mel and Enid Zuckerman College of Public Health. Dr. Watson began his research in public health at the Harvard School of Public Health as a Fellow in 1971 doing field work on vaccines in Saudi Arabia. He has done clinical studies in Colombia, Iran, Egypt, Saudi Arabia and the United States which provides a broad international view of public health. He has served in the military reserve hospital for 17 years with extensive training in medical responses to disasters as the chief biochemistry officer of a general hospital, retiring as a Lt. Colonel. He is a distinguished member of several national and international nutrition, immunology, and cancer societies. Dr. Watson’s career has involved studying many lifestyle aspects for their uses in health promotion. He has edited over 100 biomedical reference books and 450 papers and chapters. His teaching and research focuses on alcohol, tobacco, and drugs of abuse in heart function and disease in mouse models.
Affiliations and Expertise
Professor, Mel and Enid Zuckerman College of Public Health and School of Medicine, Arizona Health Sciences Center, University of Arizona, Tucson, AZ, USA
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