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Platelets: Receptors, Adhesion, Secretion, Part B - 1st Edition - ISBN: 9780121821166, 9780080883281

Platelets: Receptors, Adhesion, Secretion, Part B, Volume 215

1st Edition

Editors in Chief: John Abelson Melvin Simon
Serial Volume Editor: Jacek Hawiger
eBook ISBN: 9780080883281
Imprint: Academic Press
Published Date: 9th September 1992
Page Count: 526


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Table of Contents

Isolation of Platelet Membranes, Subcellular Organelles, and Cytoskeleton: J. Hawiger, Introduction to Platelet Structural and Functional Organization. Platelet Membranes and Granules: N. Crawford, K.S. Authi, and N. Hack, Isolation and Characterization of Platelet Membranes Prepared by Free Flow Electrophoresis. M.J. Broekman, Homogenization by Nitrogen Cavitation Technique Applied to Platelet Subcellular Fractionation. J.T. Harmon, N.J. Greco, and G.A. Jamieson, Isolation of Human Platelet Plasma Membranes by Glycerol Lysis. M.H. Fukami, Isolation of Dense Granules from Human Platelets. Platelet Cytoskeleton: J.E.B. Fox, C.C. Reynolds, and J.K. Boyles, Studying the Platelet Cytoskeleton in Triton X-100 Lysates. S. Rosenberg-Schaier, Purification and Characterization of Platelet Actin, Actin-Binding Proteins, and ~ga-Actin. J.L. Daniel and J.R. Sellers, Purification and Characterization of Platelet Myosin. J. Bryan, Isolation and Characterization of Platelet Gelsolin. N.C. Collier and K. Wang, Purification and Properties of Human Platelet P235: Talin. J.G. White, Ultrastructural Analysis of Platelet Contractile Apparatus. Platelet Receptors: Assays and Purification: J. Hawiger, Repertoire of Platelet Receptors. Receptors for Platelet Agonists: D.E. Macfarlane, 2-Methylthioadenosine [*gb-32P]Diphosphate: Synthesis and Use as Probe of Platelet ADP-Receptors. W.R. Figures, L.M. Scearce, R.F. Colman, and R.W. Colman, Interaction of Nucleotide Affinity Analog 5'p-Fluorosulfonylbenzoyladenosine with Platelet ADP Receptor: Aggregin. E.R. Simons, T.A. Davies, S.M. Greenberg-Sepersky, and N.E. Larsen, Thrombin Receptors on Human Platelets. D.R. Phillips and M.C. Berndt, Platelet Membrane Glycoprotein V Purification. S.E. Domino, M.G. Repaske, C.A. Bonner, M.E. Kennedy, S. Brandon, and L.E. Limbird, Identification, Solubilization, and Affinity Chromatography of Human Platelet ~ga2-Adrenergic Receptors. J.R. Peters, D.P. Geaney, and D.G. Grahame-Smith, 5-[3H]Hydroxytryptamine and [3H]Lysergic Acid Diethylamide Binding to Human Platelets. G. Rudnick and C.J. Humphreys, Platelet Serotonin Transporter. F.H. Valone, Quantitation of Binding of Platelet Activating Factor 1-0-Alkyl-2-acetyl-2-sn-glycero-3-phosphorylcholine to Intact Platelets and Platelet Membranes. Receptors for Adhesive Proteins: J. Hawiger and S. Timmons, Binding of Fibrinogen and von Willebrand Factor to Platelet Glycoprotein Iib-IIIa Complex. D.R. Phillips, L. Fitzgerald, L. Parise, and B. Steiner, Platelet Membrane Glycoprotein IIb-IIIa Complex: Purification, Characterization, and Reconstitution into Phospholipid Vesicles. Z.M. Ruggeri, T.S. Zimmerman, S. Russell, R. Bader, and L. DeMarco, Von Willebrand Factor Binding to Platelet Glycoprotein Ib Complex. A.N. Wicki, J.M. Clemetson, B. Steiner, W. Schnippering, and K.J. Clemetson, Isolation and Characterization of Glycoprotein Ib. J. Loscalzo and R.I. Handin, Platelet Glycocalicin. L.E. Scudder, E.L. Kalomiris, and B.S. Coller, Preparation and Functional Characterization of Monoclonal Antibodies against Glycoprotein Ib. J. Forsyth, E.F. Plow, and M.H. Ginsberg, Fibronectin Binding to Platelets. Receptors for Clotting Factors and Other Ligands: M.E. Nesheim, R.P. Tracy, P.B. Tracy, D.S. Boskovic, and K.G. Mann, Mathematical Simulation of Prothrombinase. P.B. Tracy, M.E. Nesheim, and K.G. Mann, Platelet Factor Xa Receptor. D. Sinha and P.N. Walsh, Binding of Coagulation Factor XIa to a Receptor on Human Platelets. J.S. Greengard and J.H. Griffin, High Molecular Weight Kininogen Receptor. E. Koller and F. Koller, Binding Characteristics of Homologous Plasma Lipoproteins to Human Platelets. A.S. Hajek and J.H. Joist, Platelet Insulin Receptor. General Approaches to Receptor Analysis: J.V. Staros, N.J. Kotite, and L.W. Cunningham, Membrane-Impermeant Cross-Linking Reagents for Structural and Functional Analyses of Platelet Membrane Glycoproteins. D.R. Phillips, Surface Labeling of Platelet Membrane Glycoproteins. B. Adelman, P. Carlson, and R.I. Handin, Evaluation of Platelet Surface Antigens by Fluorescence Flow Cytometry. D.S. Beardsley, Identification of Platelet Membrane Target Antigens for Human Antibodies by Immunoblotting. S. Karpatkin, S. Shulman, and L. Howard, Crossed Immunoelectrophoresis of Human Platelet Membranes. R.M. Albrecht, O.E. Olorundare, S.R. Simmons, J.C. Loftus, and D.F. Mosher, Use of Correlative Microscopy with Colloidal Gold Labeling to Demonstrate Platelet Receptor Distribution and Movement.


This volume contains a comprehensive collection of methods for the isolation of platelet membranes, subcellular organelles, the cytoskeleton, and for the assay and purification of platelet receptors.

Key Features

@introbul:Key Features @bul:* Platelets are cellular elements with the highest density of receptors per membrane surface area involved in binding of adhesive molecules, clotting, enzymes, and vasoactive amines

  • Platelets are essential for the arrest of bleeding and for formation of intravascular thrombi contributing to heart attacks, strokes, and disseminated intravascular coagulation, and are involved in immune complex disease
  • Platelets are useful for studying the processes of cell adhesion, neurotransmitter uptake, stimulus-response coupling, including signal transduction and secretion


Biochemists, all biologists, hematologists, pharmacologists, physiologists, pathologists, neurochemists, and graduate students in these disciplines.


No. of pages:
© Academic Press 1992
9th September 1992
Academic Press
eBook ISBN:


@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY

About the Editors in Chief

John Abelson

Affiliations and Expertise

California Institute of Technology, Division of Biology, Pasadena, U.S.A.

Melvin Simon

Affiliations and Expertise

The Salk Institute, La Jolla, CA, USA

About the Serial Volume Editor

Jacek Hawiger

Affiliations and Expertise

University of Birmingham