Isolation of Platelet Membranes, Subcellular Organelles, and Cytoskeleton:
J. Hawiger, Introduction to Platelet Structural and Functional Organization.
Platelet Membranes and Granules:
N. Crawford, K.S. Authi, and N. Hack, Isolation and Characterization of Platelet Membranes Prepared by Free Flow Electrophoresis.
M.J. Broekman, Homogenization by Nitrogen Cavitation Technique Applied to Platelet Subcellular Fractionation.
J.T. Harmon, N.J. Greco, and G.A. Jamieson, Isolation of Human Platelet Plasma Membranes by Glycerol Lysis.
M.H. Fukami, Isolation of Dense Granules from Human Platelets.
J.E.B. Fox, C.C. Reynolds, and J.K. Boyles, Studying the Platelet Cytoskeleton in Triton X-100 Lysates.
S. Rosenberg-Schaier, Purification and Characterization of Platelet Actin, Actin-Binding Proteins, and ~ga-Actin.
J.L. Daniel and J.R. Sellers, Purification and Characterization of Platelet Myosin.
J. Bryan, Isolation and Characterization of Platelet Gelsolin.
N.C. Collier and K. Wang, Purification and Properties of Human Platelet P235: Talin.
J.G. White, Ultrastructural Analysis of Platelet Contractile Apparatus.
Platelet Receptors: Assays and Purification:
J. Hawiger, Repertoire of Platelet Receptors.
Receptors for Platelet Agonists:
D.E. Macfarlane, 2-Methylthioadenosine [*gb-32P]Diphosphate: Synthesis and Use as Probe of Platelet ADP-Receptors.
W.R. Figures, L.M. Scearce, R.F. Colman, and R.W. Colman, Interaction of Nucleotide Affinity Analog 5'p-Fluorosulfonylbenzoyladenosine with Platelet ADP Receptor: Aggregin.
E.R. Simons, T.A. Davies, S.M. Greenberg-Sepersky, and N.E. Larsen, Thrombin Receptors on Human Platelets.
D.R. Phillips and M.C. Berndt, Platelet Membrane Glycoprotein V Purification.
S.E. Domino, M.G. Repaske, C.A. Bonner, M.E. Kennedy, S. Brandon, and L.E. Limbird, Identification, Solubilization, and Affinity Chromatography of Human Platelet ~ga2-Adrenergic Receptors.
J.R. Peters, D.P. Geaney, and D.G. Grahame-Smith, 5-[3H]Hydroxytryptamine and [3H]Lysergic Acid Diethylamide Binding to Human Platelets.
G. Rudnick and C.J. Humphreys, Platelet Serotonin Transporter.
F.H. Valone, Quantitation of Binding of Platelet Activating Factor 1-0-Alkyl-2-acetyl-2-sn-glycero-3-phosphorylcholine to Intact Platelets and Platelet Membranes.
Receptors for Adhesive Proteins:
J. Hawiger and S. Timmons, Binding of Fibrinogen and von Willebrand Factor to Platelet Glycoprotein Iib-IIIa Complex.
D.R. Phillips, L. Fitzgerald, L. Parise, and B. Steiner, Platelet Membrane Glycoprotein IIb-IIIa Complex: Purification, Characterization, and Reconstitution into Phospholipid Vesicles.
Z.M. Ruggeri, T.S. Zimmerman, S. Russell, R. Bader, and L. DeMarco, Von Willebrand Factor Binding to Platelet Glycoprotein Ib Complex.
A.N. Wicki, J.M. Clemetson, B. Steiner, W. Schnippering, and K.J. Clemetson, Isolation and Characterization of Glycoprotein Ib.
J. Loscalzo and R.I. Handin, Platelet Glycocalicin.
L.E. Scudder, E.L. Kalomiris, and B.S. Coller, Preparation and Functional Characterization of Monoclonal Antibodies against Glycoprotein Ib.
J. Forsyth, E.F. Plow, and M.H. Ginsberg, Fibronectin Binding to Platelets.
Receptors for Clotting Factors and Other Ligands:
M.E. Nesheim, R.P. Tracy, P.B. Tracy, D.S. Boskovic, and K.G. Mann, Mathematical Simulation of Prothrombinase.
P.B. Tracy, M.E. Nesheim, and K.G. Mann, Platelet Factor Xa Receptor.
D. Sinha and P.N. Walsh, Binding of Coagulation Factor XIa to a Receptor on Human Platelets.
J.S. Greengard and J.H. Griffin, High Molecular Weight Kininogen Receptor.
E. Koller and F. Koller, Binding Characteristics of Homologous Plasma Lipoproteins to Human Platelets.
A.S. Hajek and J.H. Joist, Platelet Insulin Receptor.
General Approaches to Receptor Analysis:
J.V. Staros, N.J. Kotite, and L.W. Cunningham, Membrane-Impermeant Cross-Linking Reagents for Structural and Functional Analyses of Platelet Membrane Glycoproteins.
D.R. Phillips, Surface Labeling of Platelet Membrane Glycoproteins.
B. Adelman, P. Carlson, and R.I. Handin, Evaluation of Platelet Surface Antigens by Fluorescence Flow Cytometry.
D.S. Beardsley, Identification of Platelet Membrane Target Antigens for Human Antibodies by Immunoblotting.
S. Karpatkin, S. Shulman, and L. Howard, Crossed Immunoelectrophoresis of Human Platelet Membranes.
R.M. Albrecht, O.E. Olorundare, S.R. Simmons, J.C. Loftus, and D.F. Mosher, Use of Correlative Microscopy with Colloidal Gold Labeling to Demonstrate Platelet Receptor Distribution and Movement.
This volume contains a comprehensive collection of methods for the isolation of platelet membranes, subcellular organelles, the cytoskeleton, and for the assay and purification of platelet receptors.
@introbul:Key Features @bul:* Platelets are cellular elements with the highest density of receptors per membrane surface area involved in binding of adhesive molecules, clotting, enzymes, and vasoactive amines
- Platelets are essential for the arrest of bleeding and for formation of intravascular thrombi contributing to heart attacks, strokes, and disseminated intravascular coagulation, and are involved in immune complex disease
- Platelets are useful for studying the processes of cell adhesion, neurotransmitter uptake, stimulus-response coupling, including signal transduction and secretion
Biochemists, all biologists, hematologists, pharmacologists, physiologists, pathologists, neurochemists, and graduate students in these disciplines.
- No. of pages:
- © Academic Press 1992
- 9th September 1992
- Academic Press
- eBook ISBN:
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University of Birmingham
California Institute of Technology, Division of Biology, Pasadena, U.S.A.
The Salk Institute, La Jolla, CA, USA