Description

This volume contains a comprehensive collection of methods for the isolation of platelet membranes, subcellular organelles, the cytoskeleton, and for the assay and purification of platelet receptors.

Key Features

@introbul:Key Features @bul:* Platelets are cellular elements with the highest density of receptors per membrane surface area involved in binding of adhesive molecules, clotting, enzymes, and vasoactive amines * Platelets are essential for the arrest of bleeding and for formation of intravascular thrombi contributing to heart attacks, strokes, and disseminated intravascular coagulation, and are involved in immune complex disease * Platelets are useful for studying the processes of cell adhesion, neurotransmitter uptake, stimulus-response coupling, including signal transduction and secretion

Readership

Biochemists, all biologists, hematologists, pharmacologists, physiologists, pathologists, neurochemists, and graduate students in these disciplines.

Table of Contents

Isolation of Platelet Membranes, Subcellular Organelles, and Cytoskeleton: J. Hawiger, Introduction to Platelet Structural and Functional Organization. Platelet Membranes and Granules: N. Crawford, K.S. Authi, and N. Hack, Isolation and Characterization of Platelet Membranes Prepared by Free Flow Electrophoresis. M.J. Broekman, Homogenization by Nitrogen Cavitation Technique Applied to Platelet Subcellular Fractionation. J.T. Harmon, N.J. Greco, and G.A. Jamieson, Isolation of Human Platelet Plasma Membranes by Glycerol Lysis. M.H. Fukami, Isolation of Dense Granules from Human Platelets. Platelet Cytoskeleton: J.E.B. Fox, C.C. Reynolds, and J.K. Boyles, Studying the Platelet Cytoskeleton in Triton X-100 Lysates. S. Rosenberg-Schaier, Purification and Characterization of Platelet Actin, Actin-Binding Proteins, and ~ga-Actin. J.L. Daniel and J.R. Sellers, Purification and Characterization of Platelet Myosin. J. Bryan, Isolation and Characterization of Platelet Gelsolin. N.C. Collier and K. Wang, Purification and Properties of Human Platelet P235: Talin. J.G. White, Ultrastructural Analysis of Platelet Contractile Apparatus. Platelet Receptors: Assays and Purification: J. Hawiger, Repertoire of Platelet Receptors. Receptors for Platelet Agonists: D.E. Macfarlane, 2-Methylthioadenosine [*gb-32P]Diphosphate: Synthesis and Use as Probe of Platelet ADP-Receptors. W.R. Figures, L.M. Scearce, R.F. Colman, and R.W. Colman, Interaction of Nucleotide Affinity Analog 5'p-Fluorosulfonylbenzoyladenosine with Platelet ADP Receptor:

Details

No. of pages:
526
Language:
English
Copyright:
© 1992
Published:
Imprint:
Academic Press
Print ISBN:
9780121821166
Electronic ISBN:
9780080883281

Reviews

@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY