Plant Tissue Culture

Plant Tissue Culture

Techniques and Experiments

3rd Edition - July 20, 2012

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  • Author: Roberta Smith
  • eBook ISBN: 9780124159853
  • Paperback ISBN: 9780124159204

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Description

Plant Tissue Culture, Third Edition builds on the classroom tested, audience proven manual that has guided users through successful plant culturing A.tumefaciens mediated transformation, infusion technology, the latest information on media components and preparation, and regeneration and morphogenesis along with new exercises and diagrams provide current information and examples. The included experiments demonstrate major concepts and can be conducted with a variety of plant material that are readily available throughout the year. This book provides a diverse learning experience and is appropriate for both university students and plant scientists.

Key Features

  • Provides new exercises demonstrating tobacco leaf infiltration to observe transient expression of proteins and subcellular location of the protein, and information on development of a customized protocol for protoplast isolation for other experimental systems
  • Includes detailed drawings that complement both introductions and experiments
  • Guides reader from lab setup to supplies, stock solution and media preparation, explant selection and disinfestations, and experimental observations and measurement
  • Provides the latest techniques and media information, including A. tumefaciens mediated transformation and infusion technology
  • Fully updated literature

Readership

Faculty, graduate students, and undergraduates interested in plant cell and tissue culture

Table of Contents

  • Preface

    Acknowledgments

    Scheduling and Interrelationships of Exercises

    Chapter 1. History of Plant Cell Culture

    Introduction

    The Early Years

    The Era of Techniques Development

    The Recent Past

    The Present Era

    References

    Chapter 2. Setup of a Tissue Culture Laboratory

    Work Areas

    Bibliography

    Chapter 3. Media Components and Preparation

    Inorganic Salts

    Plant Growth Regulators

    Vitamins

    Carbohydrates

    Hexitols

    Gelling Agent

    Amino Acids

    Antibiotics

    Natural Complexes

    Media pH

    Medium Preparation

    References

    Chapter 4. Explant Preparation

    Explant Age

    Season

    Explant Size

    Plant Quality

    Goal

    Genotype

    Aseptic Technique

    Aseptic Germination of Seeds

    Bibliography

    Chapter 5. Contamination

    Explant

    Personnel

    Laminar Air Flow Hood

    Insects

    Media

    Instruments

    Room Air Handling System

    References

    Chapter 6. Callus Induction

    Callus Initiation

    Explant Orientation

    Establishment of Competent Cereal Cell Cultures

    Salt Selection In Vitro

    Growth Curves

    Cellular Variation from Callus Cultures

    Bibliography

    Chapter 7. Regeneration and Morphogenesis

    Controlled Morphogenesis: Potato Tuberization

    Somatic Embryogenesis

    Regeneration of Rice

    Dormancy Requirements of Explants

    Primary Morphogenesis: Douglas Fir

    Bibliography

    Chapter 8. Woody Shrubs and Trees

    Rhododendrons and Azaleas

    Roses

    Birch Trees

    White Cedar (Arborvitae)

    References

    Chapter 9. Haploid Plants from Anther Culture

    Datura Anther Culture

    African Violet

    Tobacco

    Anther Squash Technique

    Bibliography

    Chapter 10. Embryo Rescue

    Sweet Corn Embryo Culture

    Crabapple and Pear

    Bibliography

    Chapter 11. Meristem Culture for Virus-Free Plants

    Isolation of the Shoot Apical Meristem

    Establishing Virus- and Bacteria-Free Plants

    Garlic Propagation From Bulb Scales

    Bibliography

    Chapter 12. In Vitro Propagation for Commercial Production of Ornamentals

    Boston Fern

    Staghorn Fern

    Ficus

    Kalanchoe

    African Violet

    Endangered Species

    Cactus

    Bibliography

    Chapter 13. Protoplast Isolation and Fusion

    Plant Protoplast Isolation and Fusion

    Bibliography

    Chapter 14. Agrobacterium-Mediated Transformation of Plants

    Petunia or Tobacco Leaf Disk

    Petunia Shoot Apex

    Tobacco Leaf Infiltration

    Bibliography

    Appendix I: Useful Measurements

    Length

    Temperature

    Concentration

    Weight

    Appendix II: Review of Solution Preparation

    Percentage Solutions

    Molar Solutions

    Dilution of a Stock Solution

    Appendix III: List of Suppliers

    Appendix IV: Common Plant Tissue Culture Terms

    Index

Product details

  • No. of pages: 208
  • Language: English
  • Copyright: © Academic Press 2012
  • Published: July 20, 2012
  • Imprint: Academic Press
  • eBook ISBN: 9780124159853
  • Paperback ISBN: 9780124159204

About the Author

Roberta Smith

Affiliations and Expertise

Emeritus Professor, Department of Horticulture, Vegetable Crops Improvement Center, Texas A&M University, College Station, Texas, U.S.A.

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