Table of Contents

Preface

Acknowledgments

Scheduling and Interrelationships of Exercises

Chapter 1. History of Plant Cell Culture

Introduction

The Early Years

The Era of Techniques Development

The Recent Past

The Present Era

References

Chapter 2. Setup of a Tissue Culture Laboratory

Work Areas

Bibliography

Chapter 3. Media Components and Preparation

Inorganic Salts

Plant Growth Regulators

Vitamins

Carbohydrates

Hexitols

Gelling Agent

Amino Acids

Antibiotics

Natural Complexes

Media pH

Medium Preparation

References

Chapter 4. Explant Preparation

Explant Age

Season

Explant Size

Plant Quality

Goal

Genotype

Aseptic Technique

Aseptic Germination of Seeds

Bibliography

Chapter 5. Contamination

Explant

Personnel

Laminar Air Flow Hood

Insects

Media

Instruments

Room Air Handling System

References

Chapter 6. Callus Induction

Callus Initiation

Explant Orientation

Establishment of Competent Cereal Cell Cultures

Salt Selection In Vitro

Growth Curves

Cellular Variation from Callus Cultures

Bibliography

Chapter 7. Regeneration and Morphogenesis

Controlled Morphogenesis: Potato Tuberization

Somatic Embryogenesis

Regeneration of Rice

Dormancy Requirements of Explants

Primary Morphogenesis: Douglas Fir

Bibliography

Chapter 8. Woody Shrubs and Trees

Rhododendrons and Azaleas

Roses

Birch Trees

White Cedar (Arborvitae)

References

Chapter 9. Haploid Plants from Anther Culture

Datura Anther Culture

African Violet

Tobacco

Anther Squash Technique

B

Details

No. of pages:
208
Language:
English
Copyright:
© 2013
Published:
Imprint:
Academic Press
Print ISBN:
9780124159204
Electronic ISBN:
9780124159853