Plant Tissue Culture

Plant Tissue Culture: Techniques and Experiments, Fourth Edition, builds on the classroom tested, audience proven manual that has guided users through successful plant culturing for almost 30 years. The book's experiments demonstrate major concepts and can be conducted with a variety of plant materials readily available throughout the year. This fully updated edition describes the principles of the newest technologies, including CRISPR/Cas9 gene editing and RNAi technology with plant cell and tissue cultures and their applications. Bridging the gap between theory and practice, this book contains detailed methodology supported by comprehensive illustrations, giving users a diverse learning experience for both university students and plant scientists.

• Provides fundamental principles, methods and techniques in plant cell, tissue and organ culture that can be applied to all crop plants, including agronomic crops, horticulture and forestry crops for germplasm improvement
• Guides readers from lab setup to supplies, stock solution and media preparation, explant selection and disinfestations, and experimental observations and measurement
• Contains the latest advances and updates since the previous edition published in 2012

Undergraduate and postgraduate students and researchers focusing on plant tissue culture/micro-propagation, horticulture, botany, biotechnology and plant physiology

1. History of plant tissue culture
2. Setup of a tissue culture laboratory
3. Media components and preparation
4. Explant preparation
5. Contamination
6. Callus induction
7. Regeneration and morphogenesis
8. Woody shrubs and trees
9. Haploid plants from another culture
10. Embryo rescue
11. Meristem culture for virus-free plants
12. In Vitro propagation for commercial production of ornamentals
13. Protoplast isolation and fusion
14. Agrobacterium-mediated transformation of plants
14-1. Petunia or tobacco leaf disk
14-2. Petunia shoot apex
14-3. Tobacco leaf infiltration
14-4. Arabidopsis floral dip transformation
14-5. Dicot plant-based techniques and experiments
14-6. Rice transformation techniques and experiments
15. Genome edition and RNAi
15-1. Tomato genome edition
15.2. Lettuce RNAi